Development of an Analytical Method for the Determination of Metals in Chicken Breast by Microwave Induced Plasma Optical Emission Spectrometry (MIP-OES)

Abstract

An analytical method for the determination of Al, Ca, Cd, Cr, Cu, Fe, Hg, K, Mg, Na and Zn in breast of conventional, country and Christmas chicken and turkey samples using microwave induced plasma optical emission spectrometry (MIP-OES) technique was developed. Samples were decomposed in a digester block with a reflux system under conditions optimized using a central composite design as it follows: 2 g of sample, in 7.5 mL of HNO 3 during 180 min at 160 °C. The accuracy was evaluated by the analysis of standard reference material SRM 1546 meat homogenate (94 to 108%) and also by addition and recovery tests (80 to 121%). The highest concentrations, in mg kg -1 , of Al (2.77), Ca (88.3), Cu (1.99), Fe (3.65), K (3236), Mg (292.62) and Na (312.03) were found in the breast of conventional chicken sample. Zinc presented high value in the breast of turkey sample (10.6 mg kg -1 ). However, the found concentrations were lower than the daily consumption limit established by supervisory agencies and reference limits from Brazilian legislation. Cadmium, Cr and Hg presented values below limit of quantification. In this way, it is evident that chicken breast should be consumed in a complementary way in a healthy diet.

Fit-for-Purpose MIP OES Method to Meet the Requirements of the Brazilian Regulations for K and Na in Coconut Water, and Nutritional Assessment

“…The MIP OES is a multi-elemental analytical technique that uses a nitrogen plasma as an atomizer and this is the main differential of this technique, as nitrogen is cheaper than the other gases normally used to generate the plasma in other emission or mass spectrometry techniques, such as the argon gas used in ICP OES or ICP-MS, or less dangerous than acetylene or nitrous oxide used in techniques of atomic absorption (AA). 14 Pinto et al, 15 for instance, determined the contents of Al, Ca, Cd, Cr, Cu, Fe, Hg, K, Mg, Na and Zn in chicken breast samples using MIP OES after a treatment performed in a block digester equipped with a reflux system, whereas Sá et al 16 determined trace elements in meat by MIP OES after solid-phase extraction. Infant cereal samples were analyzed for the estimation of the total content and bioaccessible fractions of thirteen elements, including essential and potentially toxic trace elements.…”