Evaluation of in vitro and in vivo therapeutic efficacy of Ribes alpestre Decne in Rheumatoid arthritis

Hasan, Alamgeer Umme Habiba

doi:10.1590/s2175-97902019000217832

Cited by 15 publications

(9 citation statements)

References 13 publications

(12 reference statements)

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“…Maintenance and stability of the lysosomal membrane remain crucial as lysosomal contents, such as bactericidal enzymes and proteases, are released during inflammation; further exacerbation can be induced by cell membrane rupture, cell disintegration, and loss of cations from the membrane. This results in denaturation and loss of protein function, which is responsible for the physical integrity and regulation of cell homeostasis in biological membranes [ 25 , 26 ].…”

Section: Discussionmentioning

confidence: 99%

In-vitro antioxidant and anti-inflammatory activities of ethanol stem-bark extract of Blighia sapida K.D. Koenig

Adekola

Areola

Fagbohun

et al. 2022

Journal of Pharmaceutical Analysis

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Section: Discussionmentioning

confidence: 99%

In-vitro antioxidant and anti-inflammatory activities of ethanol stem-bark extract of Blighia sapida K.D. Koenig

Adekola

Areola

Fagbohun

et al. 2022

Journal of Pharmaceutical Analysis

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“…Percentage inhibition of edema = (1-Vt)/Vc x 100 Where Vt and Vc are the joint diameters of treated and control rats [18].…”

Section: Extraction and Partitioningmentioning

confidence: 99%

In vitro radical scavenging and in vivo antiarthritic effects of Spondias mombin (L.) stem bark

Adekanmi,

Oguchukwu,

Tolani

et al. 2023

J. Pharm Bio.

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Rheumatoid arthritis (RA) is a systemic autoimmune disorder that affects about 1% of the population globally. Numerous conventional medications are available for the management of RA. However, their usage is limited due to the associated side effects. Spondias mombin is a member of the Anacardiaceae family known for its anti-arthritic effects. This study aimed to evaluate the anti-arthritic activity of S. mombin (L.) stem bark in RA. Radical scavenging activity was determined using DPPH assay. The anti-inflammatory effect was determined using protease inhibition and formaldehyde-induced arthritis models. S. mombin aqueous fraction (SMAF) exhibited a significant (p<0.0001) radical scavenging effect (IC50 of 57.87 µg/mL) over the S. mombin methanol extract (SMME) and S. mombin chloroform fraction (SMCF) when compared to the sham control. SMAF at 500 µg/mL significantly (p<0.05) inhibited (56.89 ± 2.02%) the effect of protease when compared to the sham control. SMME significantly (p<0.001) reduced inflammation in rat paws when compared to the control with peak effects seen at the dose of 50 mg/kg body weight. The result of the study has revealed that the stem bark has radical scavenging and anti-inflammatory effects.

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“…The mixtures were heated at 70°C for 5 minutes after incubating at 37 ± 2°C for 15 minutes. At 660 nm, the absorbance was measured [25]. Using the following formula, the percentage of protein denaturation inhibition was calculated:…”

Section: Egg Albumin Induced Protein Denaturation Assaymentioning

confidence: 99%

Assessment of the Anti-Arthritic and Immunosuppressive Potential of Enalapril by Using Network Pharmacology, Molecular Docking and Experimental Pharmacology Approaches

QASIM

2023

FARMACIA

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The present study validates the anti-arthritic and immunosuppressive attributes of enalapril, an angiotensin-converting enzyme inhibitor (ACEI) with a potential role in major inflammatory pathways. A network pharmacology-based prediction approach was utilised to reveal the possible target genes that can be targeted by enalapril for managing arthritis. In vitro anti-arthritic efficacy of enalapril was assessed using an assay that measures the denaturation of proteins in bovine serum albumin (BSA), egg albumin and the stability of the human red blood cell (HRBC) membrane at a dosage ranging from 100 to 6400 µg/mL. In vivo anti-arthritic efficacy of enalapril at doses of 2, 4 and 8 mg/kg bw was assessed in formaldehyde-induced arthritis model. Dinitrochlorobenzene (DNCB)-provoked delayed type hypersensitivity (DTH) and cyclophosphamide-provoked myelosuppression were employed to assess the immunosuppressant capacity of enalapril. Network pharmacology outcomes revealed that the anti-arthritic effects of enalapril targets include tumour necrosis factor (TNF), matrix metalloproteinase 9 (MMP-9) and caspase 3 (CASP3). Molecular docking of enalapril with these three targets also validates the strong interaction between them. Enalapril markedly inhibited protein denaturation in egg albumin and bovine serum albumin (BSA) assays and stabilised RBC haemolysis exposed to hypotonic media. Likewise, enalapril demonstrated a dose-dependent inhibition of paw oedema provoked by formaldehyde. In the DTH assay, enalapril significantly reduced skin thickness compared to the negative control group and exhibited potent immunosuppressant potential in cyclophosphamide-induced myelosuppression. Based on the outcomes of the current study, it can be predicted that enalapril has anti-arthritic attributes that might be due to its immunosuppressive potential. RezumatStudiul validează efectele antiartritice și imunosupresoare ale enalaprilului, un inhibitor al enzimei de conversie a angiotensinei (IECA) cu potențială acțiune în patologiile inflamatorii. Prin intermediul unei abordări farmacologice predictive s-au identificat potențialele gene țintă ale enalaprilului în terapia artritei. Efectul in vitro al enalaprilului a fost evaluat prin testul de denaturare a albuminei serice bovine (BSA), al albuminei din ou și de stabilizare a membranei globulelor roșii umane (HRBC) la o doză cuprinsă între 100 și 6400 µg/mL. Acțiunea in vivo a enalaprilului în doze de 2, 4 și 8 mg/kgc a fost evaluată în artrita indusă experimental cu formaldehidă. Pentru a evalua acțiunea imunosupresoare a enalaprilului a fost utilizat testul hipersensibilității întârziate (DTH), folosind dinitroclorobenzen (DNCB) și mielosupresia provocată de ciclofosfamidă. Rezultatele abordărilor farmacologice au arătat că efectul antiartritic al enalaprilului este atribuit interacțiunii cu factorul de necroză tumorală (TNF), metaloproteinaza 9 a matricei (MMP-9) și caspaza 3 (CASP3).

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Evaluation of in vitro and in vivo therapeutic efficacy of Ribes alpestre Decne in Rheumatoid arthritis

Cited by 15 publications

References 13 publications

In-vitro antioxidant and anti-inflammatory activities of ethanol stem-bark extract of Blighia sapida K.D. Koenig

In-vitro antioxidant and anti-inflammatory activities of ethanol stem-bark extract of Blighia sapida K.D. Koenig

<i>In vitro</i> radical scavenging and <i>in vivo</i> antiarthritic effects of <i>Spondias mombin</i> (L.) stem bark

Assessment of the Anti-Arthritic and Immunosuppressive Potential of Enalapril by Using Network Pharmacology, Molecular Docking and Experimental Pharmacology Approaches

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