Formulation development and evaluation of colon targeted delayed release methotrexate pellets for the treatment of colonic carcinoma

Paradkar, Mansi; Amin, Jinal

doi:10.1590/s2175-97902018000417222

Cited by 8 publications

(7 citation statements)

References 10 publications

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“…Colorectal cancer is the 2nd most common reason of death from cancer [1]. The conventional chemotherapy is not much fruitful in its treatment as absorption of drug usually occurs in the stomach and intestine after its solubilization [2][3][4]. Nowadays, there is an increased interest in herbal drugs and remedies for the treatment of chronic diseases, including cancer [5].…”

Section: Introductionmentioning

confidence: 99%

Green synthesis of silver, iron and gold nanoparticles of lycopene extracted from tomato: their characterization and cytotoxicity against COLO320DM, HT29 and Hella cell

Shejawal¹,

Randive²,

Bhinge³

et al. 2021

J Mater Sci: Mater Med

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Our study aimed at development of Silver, Iron and Gold nanoparticles of Lycopene isolated from tomato by using green synthesis technique and to evaluate its anticancer potential against colorectal and cervical cancer. Lycopene was extracted by benzene extraction method and the silver, iron and gold nanoparticles were developed by green synthesis method. 1% aqueous extract of isolated Lycopene was mixed with 1% solutions of AgNO3, FeCl3 and HAuCl4 solutions and incubated at ambient temperature for 3–4 h separately and observed for the color change which is an indicative of formation of the nanoparticles. The prepared nanoparticles were characterized by FTIR, SEM, XRD analysis and evaluated for their antimicrobial potential. The cytotoxicity studies were carried out by in vitro assay like MTT, SRB and Tryphan blue method against Colo 320 DM, HT 29, and Hella. SEM showed nanosized particles of 50–100 nm range, whereas no antimicrobial activity was exhibited by the prepared nanoparticles. In MTT assay the LyAgNP showed maximum 41.41 ± 0.4124% inhibition against COLO320DM, whereas LyGNP exhibited 41.47 ± 0.4469% inhibition against HT 29 and LyAgNP showed 40.9 ± 0.6908% inhibition against Hella cells. In SRB assay LyAgNP showed maximum 82.68 ± 1.1798% inhibition against COLO320DM, whereas LyGNP exhibited maximum 91.21 ± 0.2372% inhibition against HT29 and 87.98 ± 0.5878% inhibition against Hella cells. In tryphan blue assay against COLO320DM, HT29 and Hella cells, the maximum inhibition exhibited by the prepared nanoparticles were observed as LyGNP 83.45 ± 0.4694%, LyAgNP 88.05 ± 0.1870% and LyAgNP65.47 ± 0.4766%. We conclude that the developed nanoparticles of Lycopene exhibited potential anticancer activity against Colorectal and cervical cancer cell as compared with pure Lycopene.

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Section: Introductionmentioning

confidence: 99%

Green synthesis of silver, iron and gold nanoparticles of lycopene extracted from tomato: their characterization and cytotoxicity against COLO320DM, HT29 and Hella cell

Shejawal¹,

Randive²,

Bhinge³

et al. 2021

J Mater Sci: Mater Med

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“…4) suggested that as the concentrations of polymer ratio increased up to certain extent, it led to decrease in the % drug release rate at 6 h. The increase in concentration of crosslinking agent up to certain extent also leads to a decrease in % drug release rate at 6 h. It can be concluded that the polymeric ratio (X1) has negative effect on % drug release at 6 h. While concentration of crosslinking agent (X2) also showed negative effect on it. Both the combined effect of concentrations of polymer ratio and crosslinking agent (CaCl 2 ) have also shown a negative impact on % drug release rate at 6 h. However the quadratic responses of both X1 and X2 shown positive response towards % drug release at 6 h [66] . Optimization techniques based on factorial design generated mathematical equations using experimental data and appropriate statistical analysis and helped to select best possible model depending upon independent variables [68] .…”

Section: Able 2: Percent Weight Loss Of 5-fu Sa Fsm Pm Dpm and Bfmentioning

confidence: 92%

“…While concentration of crosslinking agent (X2) did not show much effect on it. Both the combined effect of concentrations of polymer ratio and crosslinking agent leads to positive effect on bead size [66] . The quadratic equation for BS is given as follows, % Q6 (Y3) = (Table 2A).…”

Section: Able 2: Percent Weight Loss Of 5-fu Sa Fsm Pm Dpm and Bfmentioning

confidence: 99%

“…Similarly an increased concentration of crosslinking agent led to a better DEE. Both the combined effect of polymeric ratio and concentration of crosslinking agent lead to a positive impact on DEE [66] . The quadratic equation for BS is given as follows, BS (Y2) = 963.22+48.…”

Section: Able 2: Percent Weight Loss Of 5-fu Sa Fsm Pm Dpm and Bfmentioning

confidence: 99%

“…Thus, it can be concluded that the polynomial equations fit the data satisfactorily and were valid for predicting the % DEE, BS and % Q6 for multiparticulate beads of 5-FU towards colon targeting. Thus, considering the results from 3 2 full factorial design, the batch F0 was selected as the optimized batch [66] .…”

Section: Able 2: Percent Weight Loss Of 5-fu Sa Fsm Pm Dpm and Bfmentioning

confidence: 99%

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Development and Characterization of Colon-targeting 5-Fluorouracil Multiparticulate Beads

Sarangi¹,

Rao²,

Parcha³

et al. 2020

Indian J Pharm Sci

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This investigation is related to the development and optimization of multiparticulate beads of fenugreek seed mucilage-sodium alginate containing 5-flurouracil through ionotropic gelation technique using 3 2 full factorial design generated with Design-Expert® Version11 software. The beads were developed using CaCl 2 as the crosslinking agent. The effect of ratio of fenugreek seed mucilage and sodium alginate blend and concentration of CaCl 2 on the drug encapsulation efficiency, bead size and percent cumulative drug release in 6 h was optimized by 3 2 factorial design. The beads were also characterized using field emission scanning electron microscopy, Fourier-transform infrared spectroscopy and thermal analysis. The percent drug encapsulation efficiency of all these beads was within the range of 43.91 to 85.39 % with an in vitro drug release of 33.92 to 39.23 % in 6 h. The optimized batches of beads were coated with Eudragit S100 (5, 10 and 15 % w/v) to facilitate colon targeting in a prominent way. The in vitro drug release from the coated beads (P3) in various colonic fluids followed zero-order pattern with erosion mechanism in 18 h. The average size of these beads was within the range of 895 to 1021 µm. The optimized fenugreek seed mucilage-alginate beads containing 5-flurouracil showed significant drug permeability over a prolonged period in an ex vivo permeability study through goat colon. The results indicated successful colon targeting of 5-flurouracil multiparticulate beads developed using polymeric blends containing sodium alginate and fenugreek seed mucilage in an appropriate ratio.

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Dual-drug delivery system based on the hydrogels of alginate and sodium carboxymethyl cellulose for colorectal cancer treatment

Sheng

Gao

Yin

et al. 2021

Carbohydrate Polymers

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Formulation development and evaluation of colon targeted delayed release methotrexate pellets for the treatment of colonic carcinoma

Cited by 8 publications

References 10 publications

Green synthesis of silver, iron and gold nanoparticles of lycopene extracted from tomato: their characterization and cytotoxicity against COLO320DM, HT29 and Hella cell

Green synthesis of silver, iron and gold nanoparticles of lycopene extracted from tomato: their characterization and cytotoxicity against COLO320DM, HT29 and Hella cell

Development and Characterization of Colon-targeting 5-Fluorouracil Multiparticulate Beads

Dual-drug delivery system based on the hydrogels of alginate and sodium carboxymethyl cellulose for colorectal cancer treatment

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