Green synthesis of silver, iron and gold nanoparticles of lycopene extracted from tomato: their characterization and cytotoxicity against COLO320DM, HT29 and Hella cell

Abstract

Our study aimed at development of Silver, Iron and Gold nanoparticles of Lycopene isolated from tomato by using green synthesis technique and to evaluate its anticancer potential against colorectal and cervical cancer. Lycopene was extracted by benzene extraction method and the silver, iron and gold nanoparticles were developed by green synthesis method. 1% aqueous extract of isolated Lycopene was mixed with 1% solutions of AgNO3, FeCl3 and HAuCl4 solutions and incubated at ambient temperature for 3–4 h separately and observed for the color change which is an indicative of formation of the nanoparticles. The prepared nanoparticles were characterized by FTIR, SEM, XRD analysis and evaluated for their antimicrobial potential. The cytotoxicity studies were carried out by in vitro assay like MTT, SRB and Tryphan blue method against Colo 320 DM, HT 29, and Hella. SEM showed nanosized particles of 50–100 nm range, whereas no antimicrobial activity was exhibited by the prepared nanoparticles. In MTT assay the LyAgNP showed maximum 41.41 ± 0.4124% inhibition against COLO320DM, whereas LyGNP exhibited 41.47 ± 0.4469% inhibition against HT 29 and LyAgNP showed 40.9 ± 0.6908% inhibition against Hella cells. In SRB assay LyAgNP showed maximum 82.68 ± 1.1798% inhibition against COLO320DM, whereas LyGNP exhibited maximum 91.21 ± 0.2372% inhibition against HT29 and 87.98 ± 0.5878% inhibition against Hella cells. In tryphan blue assay against COLO320DM, HT29 and Hella cells, the maximum inhibition exhibited by the prepared nanoparticles were observed as LyGNP 83.45 ± 0.4694%, LyAgNP 88.05 ± 0.1870% and LyAgNP65.47 ± 0.4766%. We conclude that the developed nanoparticles of Lycopene exhibited potential anticancer activity against Colorectal and cervical cancer cell as compared with pure Lycopene.

Learning From Plants: Lycopene Additive Passivation toward Efficient and “Fresh” Perovskite Solar Cells with Oxygen and Ultraviolet Resistance

“…The LCP shows characteristic stretching vibrations of the CC bond at 1630 cm −1 . [27] When mixing PbI 2 with LCP, the vibration signal of CC is weakened and shifts to a higher wavenumber (12 nm) demonstrating the Lewis acid-base interaction between PbI 2 and LCP, which is in correspondence with the UV-vis absorption results. The new vibration at 707 cm −1 derives from R 2 CCR bonds in LCP [27] and the CI bond at 671 cm −1 was observed when mixing PbI 2 with LCP.…”

“…[ 27 ] When mixing PbI 2 with LCP, the vibration signal of CC is weakened and shifts to a higher wavenumber (12 nm) demonstrating the Lewis acid–base interaction between PbI 2 and LCP, which is in correspondence with the UV–vis absorption results. The new vibration at 707 cm −1 derives from R 2 CCR bonds in LCP [ 27 ] and the CI bond at 671 cm −1 was observed when mixing PbI 2 with LCP. The CI characteristic peak ( 1 H located at 4.07 ppm) is also observed in the nuclear magnetic resonance (NMR) spectroscopy as shown in Figure S6 (Supporting Information).…”

Cytotoxic and anticancer activity of a novel synthesized tet-AuNPs simultaneously activates p53 and inhibits NF-kB signaling in SKBR3 cell line

“…Further studies may be needed by other researchers. In addition, cell-dependent cytotoxicity is a particular characteristic of nanomaterials, which has been earlier reported for HT29 and HeLa [19], MCF-7 [20], and A459 [21] cancer cells. The programmed cell death occurred via apoptosis as indicated by the intrinsic and extrinsic pathways [22].…”

Herbal Liposomes: Natural Network for Targeted Drug Delivery System

“…Plant or plant preparations have been commonly used in medicine since ancient times. Up to now, phytomedicines have been commonly used in most of the world's population [4]. Herbal medicines are believed to be as old as human beings.…”