Immunostimulation and increase of intestinal lactic acid bacteria with dietary mannan-oligosaccharide in Nile tilapia juveniles

Pereira, Newton Narciso; Yasui, George Shigueki; Cardozo, Marita Vedovelli; Neto, José Dias de Souza; Farias, Thaís Heloísa Vaz; Sakabe, Róberson; Pádua, Santiago Benites de; Pilarski, Fabiana

doi:10.1590/rbz4720170006

Cited by 10 publications

(9 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This was an unexpected result since galactomannan-oligosaccharides are complex and indigestible carbohydrates (dietary fiber) that can be used as a substrate by LAB. Previous studies using similar feed additives reported an increase in LAB number in sea bream (Sparus aurata), Nile tilapia (O. niloticus), and rainbow trout (O. mykiss) gut microbiota [18,[85][86]. Similarly, an enrichment in Lactobacillus genus was found in the intestine of trout fed with a diet containing insect meal, which is rich in chitin, a mucopolysaccharide polymer, which acts as a prebiotic being hardly digested by many fish species [48].…”

Section: Plos Onementioning

confidence: 89%

Assessment of dietary supplementation with galactomannan oligosaccharides and phytogenics on gut microbiota of European sea bass (Dicentrarchus Labrax) fed low fishmeal and fish oil based diet

et al. 2020

View full text Add to dashboard Cite

There is an increasing interest from the aquafeed industry in functional feeds containing selected additives that improve fish growth performance and health status. Functional feed additives include probiotics, prebiotics, organic acids, and phytogenics (substances derived from plants and their extracts). This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet. Three experimental diets and a control diet (plant-based formulation with 10% FM and 6% FO) were tested in a 63-days feeding trial. To analyze the microbiota associated to feeds and the intestinal autochthonous (mucosa-adhered) and allochthonous (transient) microbial communities, the Illumina MiSeq platform for sequencing of 16S rRNA gene and QIIME2 pipeline were used. Metabarcoding analysis of feed-associated bacteria showed that the microbial communities of control (CTRL) feed deeply differed from those of experimental diets. The number of reads was significantly lower in CTRL feed than in other feeds. The OTU (operational taxonomic unit) number was instead similar between the feeds, ranging from 42 to 50 OTUs. The variation of resident gut microbiota induced by diet was lower than the variation of transient intestinal microbiota, because feedstuffs are a major source of allochthonous bacteria, which can temporarily integrate into the gut transient microbiome. However, the composition of transient bacterial communities was not simply a mirror of feed-borne bacteria. Indeed, the microbial profile of feeds was different from both faecal and mucosa profiles. Our findings suggest that the dietary inclusion of GMOS (0.5%) and PHYTO (0.02%) in a low FM and FO diet induces changes in gut microbiota composition of European sea bass. However, if on allochthonous microbiota the combined inclusion of GMOS and PHYTO showed an antagonistic effect on bactericidal activity

show abstract

Section: Plos Onementioning

confidence: 89%

Assessment of dietary supplementation with galactomannan oligosaccharides and phytogenics on gut microbiota of European sea bass (Dicentrarchus Labrax) fed low fishmeal and fish oil based diet

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The zootechnical indexes of tilapia did not differ significantly between treatments (p > 0.05), and the final weight was consistent with the slaughter weight of this species when farmed in net cages. Recent studies have been carried out to evaluate the effects of diet on the hematological, immunological, and histological parameters or the gut microbiota of Nile tilapia [5,8,[35][36][37][38][39][40][41], which have contributed to our understanding of the effects of dietary constituents on different physiological aspects of this species. However, no study to date has evaluated these variables in an integrated manner and/or in real farming situations.…”

Section: Discussionmentioning

confidence: 99%

“…In Brazil, the production of this species represented approximately half of all aquaculture production in 2017, which was approximately 559 thousand tons [2]. However, due to the rapid expansion and intensification of Nile tilapia production, the high densities adopted in farming systems have become a challenge, since they are a stress factor for the fish that can cause economic losses [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Effects of dietary supplementation with a microalga (Schizochytrium sp.) on the hemato-immunological, and intestinal histological parameters and gut microbiota of Nile tilapia in net cages

et al. 2020

View full text Add to dashboard Cite

Nutritional improvements in intensive aquaculture production systems is necessary for the reduction of stress, maximum utilization of nutritional components, and expression of the genetic potential of fish. The objective of this study was to evaluate the hemato-immunological, and histological parameters and gut microbiota of Nile tilapia fed with the microalga Schizochytrium sp. Males of Nile tilapia were distributed among eight net cages (6 m 3 ), and fed for 105 days with two diets: control (CON), without Schizochytrium sp., and supplemented (SUP), with 1.2% Schizochytrium sp. in the diet. The final weight, mortality, hematocrit, total erythrocyte count (RBC), hemoglobin, hematimetric indices, white blood cell count (WBC), total protein, and serum lysozyme were measured. Alterations in intestinal morphology were evaluated. The gut microbiota was evaluated with next-generation sequencing. No significant differences (p>0.05) were found in the final weight and mortality between diets. Regarding the hematological parameters, a difference (p<0.05) was detected only in RBC, with there being lower values in the SUP, although this group also showed a tendency toward having an increased mean corpuscular hemoglobin level. There were no differences (p>0.05) in total protein and serum lysozyme concentrations or in WBCs between diets, except for lymphocytes, which presented lower values (p<0.05) in the SUP, suggesting immunomodulation by the polyunsaturated fatty acids present in the microalga. There was no difference (p>0.05) in the intestinal morphology between diets. Metagenomic data indicated greater richness (represented by the Chao index) and a higher abundance of the bacterial phylum Firmicutes in the gut microbiota of the tilapia fed with the SUP diet, demonstrating that the digestion and use of the components of the microalga could influence the microbial community. The results indicated that the microalga had modulatory PLOS ONE | https://doi.

show abstract

“…and Pseudomonas sp. (Gómez and Balcázar, 2008;Levy-Pereira et al, 2018). Moreover, the mannose present in MOS particles can be recognized by the innate immune system through the mannose binding lectin (MBL), immunomodulating several immune responses (Torrecillas et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Dietary use of MOS has been reported enhancing innate immune parameters in fish, such as white blood cell numbers, phagocytosis, lysozyme and reactive oxygen species (ROS) (Song et al, 2014), and also increasing growth and survival in Dicentrarchus labrax (Torrecillas et al, 2007;, Oncorhynchus mykiss (Staykov et al, 2007), Labeo rohita (Andrews et al, 2009) and Piaractus mesopotamicus (Soares et al, 2018). However, according to the consulted literature, the level of inclusion of MOS in fish diets vary due to the species and few are the studies presenting substantial results in health of Nile tilapia (Sado et al, 2008;Selim and Reda, 2015;Levy-Pereira et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Dietary Mannan-Oligosaccharide Increases Reactive Oxigen Species Production but Decreases Serum Lysozyme in High Levels of Inclusion for Nile Tilapia

Pereira¹,

Sousa²,

Sakabe³

et al. 2020

Bol. Inst. Pesca

Self Cite

View full text Add to dashboard Cite

In the present experiment, the effects of mannan-oligosaccharide (MOS) on health and growth of Nile tilapia juveniles were investigated. In addition to the control treatment (without MOS),three levels of MOS were included in Nile tilapia diets (1, 8, and 15 g kg−1), and hematology, reactive oxygen species (ROS) production, lysozyme and productive parameters were analyzed. Fish blood was sampled at day zero (basal sample) and after 60 days of trial, and the productive parameters were evaluated at the end of the experiment. MOS feeding decreased the feed consumption (p = 0.0299) in fish fed with 1 and 8 g kg-1, but without any alteration in weight gain (WG) and feed conversion ratio (FCR). No changes were observed in the hematology due to MOS feeding after 60 days. However, the prebiotic caused changes in the innate immunity of fish, giving rise to ROS production in fish fed with 1 g kg-1 (p<0.0001) and decreasing the serum lysozyme activity of fish fed with 15 g kg-1 (p<0.0001). In conclusion, the authors recommend the inclusion of 1 g kg-1 for Nile tilapia juveniles feeding due to the positive effect in innate immune system.

show abstract

Immunostimulation and increase of intestinal lactic acid bacteria with dietary mannan-oligosaccharide in Nile tilapia juveniles

Cited by 10 publications

References 33 publications

Assessment of dietary supplementation with galactomannan oligosaccharides and phytogenics on gut microbiota of European sea bass (Dicentrarchus Labrax) fed low fishmeal and fish oil based diet

Assessment of dietary supplementation with galactomannan oligosaccharides and phytogenics on gut microbiota of European sea bass (Dicentrarchus Labrax) fed low fishmeal and fish oil based diet

Effects of dietary supplementation with a microalga (Schizochytrium sp.) on the hemato-immunological, and intestinal histological parameters and gut microbiota of Nile tilapia in net cages

Dietary Mannan-Oligosaccharide Increases Reactive Oxigen Species Production but Decreases Serum Lysozyme in High Levels of Inclusion for Nile Tilapia

Contact Info

Product

Resources

About