Identification of Methicillin-Resistant Staphylococcus aureus in Bulk Tank Milk

Keyvan, Erhan; Yurdakul, Özen; Demirtaş, Ahu; Yalçin, Halil; Bilgen, Nüket

doi:10.1590/fst.35818

Cited by 22 publications

(16 citation statements)

References 66 publications

(75 reference statements)

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“…(24), Salmonella spp. (8,57), Staphylococcus aureus (8,25,34), Bacillus cereus (40), Escherichia coli (8,36), Listeria monocytogenes (8,16,38) and Campylobacter spp. (5,20,58) were isolated from milk samples in different geographical regions.…”

Section: Resultsmentioning

confidence: 99%

Prevalence and antimicrobial susceptibility of Listeria spp. isolated from bulk raw milk in north-eastern Poland

Sołtysiuk¹,

Wiszniewska-Łaszczych²,

Wojtacka³

et al. 2022

Medycyna Weterynaryjna

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Purpose of research: The aim of the study was to determine the presence of Listeria spp. strains in the milk samples obtained from dairy farms in north-eastern Poland and to determine the profile of resistance to antibiotics recommended in the treatment of listeriosis. Material and methods: 500 samples of bulk milk were analyzed. Milk samples were obtained from dairy farms located in Warmia and Mazury region in Poland. Chronic mastitis, requiring frequent and long-term use of antibiotics has been documented in these herds. Isolation of Listeria spp. was performed according to the standard procedure PN-EN ISO 11290-1: 2017-07. Antibiotic resistance testing was performed by the disc diffusion method according to the Clinical & Laboratory Standards Institute (CLSI) recommendations. Results: In total, out of 500 samples of pooled milk, based on biochemical properties, 8 isolates were confirmed as belonging to the genus Listeria (1.6%). The further identification of Listeria strains on the basis of MicrobactListeria12L showed that 3 strains (3/8, 37.5%) belonged to L. monocytogenes species and 5 strains (5/8, 62.5%) belonged to L. innocua species. The analysis of sensitivity to commonly used antimicrobial agents showed that all isolates, both belonging to L. monocytogenes and L. innocua species, were sensitive to ampicillin. Multidrug resistance, defined as resistance to at least three classes of antibiotics, was confirmed among four isolates (50%). Research summary: The studies undertaken revealed that raw milk can pose a risk for public health due to the prevalence of pathogenic Listeria spp. among which multidrug resistant strains are present. It is therefore necessary to rationalize the use of antibiotics and to monitor bacterial resistance in the food production environment.

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Section: Resultsmentioning

confidence: 99%

Prevalence and antimicrobial susceptibility of Listeria spp. isolated from bulk raw milk in north-eastern Poland

Sołtysiuk¹,

Wiszniewska-Łaszczych²,

Wojtacka³

et al. 2022

Medycyna Weterynaryjna

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“…Jahan et al [32] reported that the prevalence of S. aureus was 25.53%, and Pexara et al [33] reported that the overall occurrence of S. aureus in milk was 24.3%, and Ameen et al [34] found a prevalence of 30%. Many studies have detected a higher occurrence of S. aureus such as 62.0% [35], 36.3% [36], and 37.32% [37]. The variation in frequencies of S. aureus in milk may be because of improper or suboptimal hygiene or poor farm management [38].…”

Section: Resultsmentioning

confidence: 99%

“…Globally, the misuse and unsupervised use of antimicrobial agents in the treatment of animal and human infections has contributed to the emergence of antimicrobial resistance [20,37].…”

Section: Resultsmentioning

confidence: 99%

Molecular detection of Staphylococcus aureus enterotoxin genes isolated from mastitic milk and humans in El-Behira, Egypt

et al. 2021

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Background and Aim: Milk is a chief source of many nutrients. However, we must also bear in mind that it is a potential source for many cases of food poisoning. This study was conducted to investigate the prevalence of cow mastitis and evaluate the presence of enterotoxins and antibiotic resistance patterns in Staphylococcus aureus isolated from milk and contact humans in El-Behira Province, Egypt. Materials and Methods: A total of 680 milk samples from 170 cows and 86 human samples consisting of 43 hand swabs and 43 nasal swabs were analyzed. The milk samples were subjected to the California mastitis test. Results: The general occurrence was 23.1% (157/680) where 48 quarters had clinical mastitis and 109 had subclinical mastitis. Subsequently, S. aureus was isolated in Baird-Parker agar where typical and atypical colonies were selected and submitted to coagulase and complementary tests. Out of 48 samples of mastitic milk studied, 16 (33.3%) showed contamination by S. aureus whereas 109 samples of subclinical mastitis showed contamination in only 18 (16.5%). On the opposite hand, of the 86 human samples, 33 revealed S. aureus contamination, corresponding to 38.37% of the samples. Furthermore, multiplex polymerase chain reaction targeting nuc and the staphylococcal enterotoxin-encoding genes sea, seb, sec, sed, and see were performed after culture, revealing that 88.2% (30/34) of milk samples and 93.9% (31/33) of human samples were variably positive to those genes. Conclusion: The use of nuc gene based PCR is an accurate and rapid method for S aureus isolates detection. A high prevalence of multiple drug-resistant isolates of S. aureus recovered from both human and milk represents further evidence for possible veterinary hazards as well as public hazards, especially to those that consume milk from this region.

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“…Bacteria resistant to several antibiotics have also been isolated from samples collected from cattle and poultry (Lee, 2006;McEachran et al, 2015), as well as from food products derived from them, i.e. meat (Kitai et al, 2005;Normanno and in, 2007), milk, and dairy products (Peles et al, 2007;Keyvan et al, 2020). Wasyl et al (2013) emphasize that the level of intake of antimicrobials has a marked effect on the number of drug-resistant bacteria isolated, and therefore resistance is most widespread in broiler and turkey housing and less prevalent in cowsheds.…”

Section: Bacteria Of the Generamentioning

confidence: 99%

Microbial contamination of the air in livestock buildings as a threat to human and animal health – a review

Chmielowiec-Korzeniowska

Trawińska

Tymczyna

et al. 2021

Annals of Animal Science

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Livestock buildings are often contaminated with bacterial and fungal microflora. Animals living in the buildings, especially their excreta and secretions and their feed, can be a source of microorganisms, including pathogens. Significant microbial contamination occurs in pig houses, poultry houses and cowsheds. The microbes most frequently isolated from the air of these buildings are bacteria of the genera Streptococcus, Staphylococcus, Bacillus, and Clostridium and of the family Enterobacteriaceae. Among fungi, the most common are Aspergillus, Trichoderma, Penicillium, Cladosporium and Alternaria. Microbes present in livestock buildings often pose a hazard to workers, in whom they can cause infectious and allergic diseases, especially respiratory disease. Bacterial endotoxins may also pose a threat to humans and animals. For this reason it is important to carry out microbiological monitoring and preventive measures on livestock farms and to maintain appropriate environmental conditions. This will reduce microbiological contamination of livestock buildings and improve both workers’ health and animal welfare.

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Identification of Methicillin-Resistant Staphylococcus aureus in Bulk Tank Milk

Cited by 22 publications

References 66 publications

Prevalence and antimicrobial susceptibility of Listeria spp. isolated from bulk raw milk in north-eastern Poland

Prevalence and antimicrobial susceptibility of Listeria spp. isolated from bulk raw milk in north-eastern Poland

Molecular detection of Staphylococcus aureus enterotoxin genes isolated from mastitic milk and humans in El-Behira, Egypt

Microbial contamination of the air in livestock buildings as a threat to human and animal health – a review

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