2022
DOI: 10.1590/fst.19921
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Identification of microflora and lactic acid bacteria in pado, a fermented fish product prepared with dried Pangium edule seed and grated coconut

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Cited by 3 publications
(2 citation statements)
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“…Bacterial genome extraction kit (Tiangen, China) was used to extract, isolate and purify the genomic DNA. Bacterial universal primers (the primers 27F and 1492R) were used for PCR amplification of 16S rRNA sequences (Syafitri et al, 2022). The synthesis of primers and the sequencing of PCR products were entrusted to Shanghai Sunny Biotechnology Co., Ltd.…”
Section: Bacterial 16s Rrna Gene Sequencing and Construction Of Phylo...mentioning
confidence: 99%
“…Bacterial genome extraction kit (Tiangen, China) was used to extract, isolate and purify the genomic DNA. Bacterial universal primers (the primers 27F and 1492R) were used for PCR amplification of 16S rRNA sequences (Syafitri et al, 2022). The synthesis of primers and the sequencing of PCR products were entrusted to Shanghai Sunny Biotechnology Co., Ltd.…”
Section: Bacterial 16s Rrna Gene Sequencing and Construction Of Phylo...mentioning
confidence: 99%
“…The enumeration of Staphylococcus was performed according to ISO 6888-1 on Baird Parker agar incubated at 37°C/24 and 48 hrs 12 . The enumeration of Lactic Acid Bacteria (LAB) was performed according to ISO 15214 on MRS agar incubated at 30°C/72 hrs in anaerobic conditions 13 . A Confirmation of LAB was performed by Gram stain and catalase test, followed by microscopic examination.…”
Section: Sample Collection and Preparationmentioning
confidence: 99%