Simultaneous quantification of phenolic acids and flavonoids in Chamaerops humilis L. using LC–ESI-MS/MS

Bouhafsoun, Aicha; Yılmaz, Mustafa Abdullah; Boukeloua, Ahmed; Temel, Hamdi; Harche, M.

doi:10.1590/fst.19917

Cited by 34 publications

(11 citation statements)

References 19 publications

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“…In the literature, the phytochemical properties of C. humilis are not well characterized, although several studies have reported the presence of tannins, flavonoids, saponins, sterols and terpenoids [ 10 ]. These results are similar to those found in samples from Algeria [ 23 ]. Notably, saponosides, responsible for many pharmacological properties, e.g., anti-inflammatory [ 24 , 25 ], were also detected in the extract of C. humilis .…”

Section: Resultssupporting

confidence: 91%

Elucidation of Antioxidant Compounds in Moroccan Chamaerops humilis L. Fruits by GC–MS and HPLC–MS Techniques

et al. 2021

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The aim of this study was to characterize the phytochemical content as well as the antioxidant ability of the Moroccan species Chamaerops humilis L. Besides crude ethanolic extract, two extracts obtained by sonication using two solvents with increased polarity, namely ethyl acetate (EtOAc) and methanol-water (MeOH-H2O) 80:20 (v/v), were investigated by both spectroscopy and chromatography methods. Between the two extracts, the MeOH-H2O one showed the highest total polyphenolic content equal to 32.7 ± 0.1 mg GAE/g DM with respect to the EtOAc extract (3.6 ± 0.5 mg GAE/g DM). Concerning the antioxidant activity of the two extracts, the EtOAc one yielded the highest value (1.9 ± 0.1 mg/mL) with respect to MeOH-H2O (0.4 ± 0.1 mg/mL). The C. humilisn-hexane fraction, analyzed by GC–MS, exhibited 69 compounds belonging to different chemical classes, with n-Hexadecanoic acid as a major compound (21.75%), whereas the polyphenolic profile, elucidated by HPLC–PDA/MS, led to the identification of a total of sixteen and thirteen different compounds in both EtOAc (major component: ferulic acid: 104.7 ± 2.52 µg/g) and MeOH-H2O extracts (major component: chlorogenic acid: 45.4 ± 1.59 µg/g), respectively. The attained results clearly highlight the potential of C. humilis as an important source of bioactive components, making it a valuable candidate to be advantageously added to the daily diet. Furthermore, this study provides the scientific basis for the exploitation of the Doum in the food, pharmaceutical and nutraceutical industries.

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Section: Resultssupporting

confidence: 91%

Elucidation of Antioxidant Compounds in Moroccan Chamaerops humilis L. Fruits by GC–MS and HPLC–MS Techniques

et al. 2021

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“…The active fraction was finally determined through the bioactivity screening process, and the components contained in B. pilosa extract were analyzed and identified. Six compounds, namely, protocatechuic acid (1), isookanin (2), (−)-quinic acid (3), 3-O-caffeoylquinic acid (4), 4-O-caffeoylquinic acid (5), and 5-O-caffeoylquinic acid (6) were separated in the aqueous extract through the extraction process and identified by HR-ESI MS/MS analysis (Table 1 and Figures S1, S3, S4 of the Supplementary Materials) [28,29]. Among these ingredients, isookanin was confirmed to be effective in anti-inflammatory activity and was finally determined as an active ingredient.…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory Activity and Mechanism of Isookanin, Isolated by Bioassay-Guided Fractionation from Bidens pilosa L.

et al. 2021

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Bidens pilosa L. (Asteraceae) has been used historically in traditional Asian medicine and is known to have a variety of biological effects. However, the specific active compounds responsible for the individual pharmacological effects of Bidens pilosa L. (B. pilosa) extract have not yet been made clear. This study aimed to investigate the anti-inflammatory phytochemicals obtained from B. pilosa. We isolated a flavonoids-type phytochemical, isookanin, from B. pilosa through bioassay-guided fractionation based on its capacity to inhibit inflammation. Some of isookanin’s biological properties have been reported; however, the anti-inflammatory mechanism of isookanin has not yet been studied. In the present study, we evaluated the anti-inflammatory activities of isookanin using lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. We have shown that isookanin reduces the production of proinflammatory mediators (nitric oxide, prostaglandin E2) by inhibiting the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-stimulated macrophages. Isookanin also inhibited the expression of activator protein 1 (AP-1) and downregulated the LPS-induced phosphorylation of p38 mitogen-activated protein kinase (MAPK) and c-jun NH2-terminal kinase (JNK) in the MAPK signaling pathway. Additionally, isookanin inhibited proinflammatory cytokines (tumor necrosis factor-a (TNF-α), interleukin-6 (IL-6), interleukin-8 (IL-8), and interleukin-1β (IL-1β)) in LPS-induced THP-1 cells. These results demonstrate that isookanin could be a potential therapeutic candidate for inflammatory disease.

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“…The previous studies on C. humilis showed that the major components in leaflet and fruit extracts were quinic, malic and chlorogenic acids. In addition, nine minor acidic components were identified [32].…”

Section: Introductionmentioning

confidence: 99%

Extraction and chemical characterization of novel water-soluble polysaccharides from two palm species and their antioxidant and antitumor activities

Dawood

Elmongy

Negm

et al. 2020

Egyptian Journal of Basic and Applied Sciences

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Two crude heteropolysaccharides were isolated from fruits of Caryota mitis and Chamaerops humilis in percentages of 2.65 and 3.89, respectively. In the case of Caryota mitis (CM) polysaccharide, it consisted of galactose, glucose and mannose as the predominant monosaccharides. While mannose, galactose and arabinose were respectively the predominant monosaccharides in the structure of Chamaerops humilis (CH) crude polysaccharide. Sixteen common amino acids were recognized to be components of the protein-bound polysaccharides of CM and CH. The major amino acid in CM and CH polysaccharides was tyrosine (1.091ng/g) and arginine (0.684 ng/g), respectively. Antioxidant activities of tested polysaccharides were examined by measuring its scavenging ability on 2,2-diphenyl-1-picrylhydrazyl and ferric reducing activity power assay. In a concentration of 1 mg/ml, CM and CH polysaccharides recorded FRAP values of 0.56 and 0.44 µmol Fe ++ /g, respectively. The scavenging activity of CM sample was higher (IC 50 , 422.11 µg/ml) than that of CH polysaccharide (IC 50 , 630.0 µg/ml). Also, the antitumor activity of CM and CH polysaccharides was investigated using HepG2 and MCF-7 human cell lines. The polysaccharide of Caryota mitis had a higher antitumor activity than that of CHPs; it had IC 50 values of 13.6 and 46.4 µg/ml in HepG2 and MCF-7 assays, respectively.

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Simultaneous quantification of phenolic acids and flavonoids in Chamaerops humilis L. using LC–ESI-MS/MS

Cited by 34 publications

References 19 publications

Elucidation of Antioxidant Compounds in Moroccan Chamaerops humilis L. Fruits by GC–MS and HPLC–MS Techniques

Elucidation of Antioxidant Compounds in Moroccan Chamaerops humilis L. Fruits by GC–MS and HPLC–MS Techniques

Anti-Inflammatory Activity and Mechanism of Isookanin, Isolated by Bioassay-Guided Fractionation from Bidens pilosa L.

Extraction and chemical characterization of novel water-soluble polysaccharides from two palm species and their antioxidant and antitumor activities

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