Elucidation of Antioxidant Compounds in Moroccan Chamaerops humilis L. Fruits by GC–MS and HPLC–MS Techniques

Abstract

The aim of this study was to characterize the phytochemical content as well as the antioxidant ability of the Moroccan species Chamaerops humilis L. Besides crude ethanolic extract, two extracts obtained by sonication using two solvents with increased polarity, namely ethyl acetate (EtOAc) and methanol-water (MeOH-H2O) 80:20 (v/v), were investigated by both spectroscopy and chromatography methods. Between the two extracts, the MeOH-H2O one showed the highest total polyphenolic content equal to 32.7 ± 0.1 mg GAE/g DM with respect to the EtOAc extract (3.6 ± 0.5 mg GAE/g DM). Concerning the antioxidant activity of the two extracts, the EtOAc one yielded the highest value (1.9 ± 0.1 mg/mL) with respect to MeOH-H2O (0.4 ± 0.1 mg/mL). The C. humilisn-hexane fraction, analyzed by GC–MS, exhibited 69 compounds belonging to different chemical classes, with n-Hexadecanoic acid as a major compound (21.75%), whereas the polyphenolic profile, elucidated by HPLC–PDA/MS, led to the identification of a total of sixteen and thirteen different compounds in both EtOAc (major component: ferulic acid: 104.7 ± 2.52 µg/g) and MeOH-H2O extracts (major component: chlorogenic acid: 45.4 ± 1.59 µg/g), respectively. The attained results clearly highlight the potential of C. humilis as an important source of bioactive components, making it a valuable candidate to be advantageously added to the daily diet. Furthermore, this study provides the scientific basis for the exploitation of the Doum in the food, pharmaceutical and nutraceutical industries.

Comparative Study on Phenolic Profile and Biological Activities of the Aerial Parts of Sinapis pubescens L. subsp. pubescens (Brassicaceae) Wild from Sicily (Italy)

“…The flower, leaf and stem hydroalcoholic extracts of S. pubescens subsp. pubescens were analyzed by high‐performance liquid chromatography coupled to a photodiode array and electrospray ionization mass spectrometry HPLC‐PDA/ESI‐MS [44–46] …”

“…The flower, leaf and stem hydroalcoholic extracts of S. pubescens subsp. pubescens were analyzed by high-performance liquid chromatography coupled to a photodiode array and electrospray ionization mass spectrometry HPLC-PDA/ESI-MS. [44][45][46] Sample preparation: The dried extracts were redissolved in 70 % MeOH. For the chromatographic separation, an injection volume of 5 μL was employed, and the analysis was performed in triplicate.…”

Flavonoid Composition and Antibacterial Properties of Crocus sativus L. Petal Extracts

“…Chromatographic separations were carried out on Ascentis Express RP C18 columns (150 × 4.6 mm; 2.7 µm) (Merck Life Science, Merck KGaA, Darmstadt, Germany) [47][48][49]. The employed mobile phase was composed of two solvents: water (solvent A) and ACN (solvent B), both acidified with 0.10% formic acid v/v.…”

Determination of the polyphenolic content of Ammodaucus leucotrichus Cosson and Durieu by liquid chromatography coupled with mass spectrometry and evaluation of the antioxidant and antiglycation properties

“…The anti-free radical activity was determined using the 2,2diphenyl-1-picrylhydrazyl method according to a method earlier described [19][20][21][22]. Briefly, different concentrations of extracts (25 μl) were added to 2 ml of 2,2-diphenyl-1-picrylhydrazyl (6.25 × 10 −5 M) solution.…”