Square-wave voltammetric determination of rutin in pharmaceutical formulations using a carbon composite electrode modified with copper (II) phosphate immobilized in polyester resin

Abstract

A carbon composite electrode modified with copper (II) phosphate immobilized in a polyester resin (Cu 3 (PO 4 ) 2 -Poly) for the determination of rutin in pharmaceutical samples by square-wave voltammetry is described herein. The modified electrode allows the determination of rutin at a potential (0.20 V vs. Ag/ AgCl (3.0 mol L -1 KCl)) lower than that observed at an unmodified electrode. The peak current was found to be linear to the rutin concentration in the range from 9.9 × 10 -8 to 2.5 × 10 -6 mol L -1 , with a detection limit of 1.2×10-8 mol L -1 . The response of the electrode was stable, with no variation in baseline levels within several hours of continuous operation. The surface morphology of the modified electrode was characterized by scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) system. The results obtained are precise and accurate. In addition, these results are in agreement with those obtained by the chromatographic method at a 95% confidence level.Uniterms: Rutin/determination in pharmaceuticals samples. Square-wave voltammetry/determination of rutin. Modified carbon composite electrode/application.Descreve-se um eletrodo de carbono modificado com fosfato de cobre (II) imobilizado em uma resina de poliéster (Cu 3 (PO 4 ) 2 -Poly) para a determinação de rutina em amostras farmacêuticas por voltametria de onda quadrada. O eletrodo modificado permite a determinação de rutina em potencial (0.20 V vs Ag / AgCl (3,0 mol L -1 KCl)) menor que o observado em um eletrodo não modificado. Verificou-se que a corrente de pico foi linear com a concentração de rutina na faixa de 9,9 × 10 -8 a 2,5 × 10 -6 mol L -1, com um limite de detecção de 1,2 × 10 -8 mol L 1. A resposta do eletrodo foi estável, sem variação significativa dentro de várias horas de operação contínua. A morfologia da superfície do eletrodo modificado foi caracterizada por microscopia eletrônica de varredura (MEV) e pelo sistema de energia dispersiva de raios-X (EDX). Os resultados obtidos foram precisos e exatos. Ademais, estes resultados estão de acordo com aqueles obtidos pelo método cromatográfico a um nível de confiança de 95%.Uniterms: Rutina/determinação em amostras farmacêuticas. Voltametria de onda quadrada/determinação de rutina. Eletrodo compósito de carbono modificado/aplicação.

NiCo2O4 Nano-/Microstructures as High-Performance Biosensors: A Review

“…During electro-oxidation, rutin is converted into 3′,4′-diquinone with the release of two H + ions and two electrons (Eq. 42 ) [ 306 , 307 ]. …”

Recent Development of Nano-Carbon Material in Pharmaceutical Application: A Review

“…Apetrei et al [ 119 ] developed sensors to detect routines. Rutin is a flavonoid glycoside that is widely used as a pharmacologically active substance with antibacterial, antioxidant, tiviral, antitumor, and so on properties [ 120 , 121 , 122 ]. This sensor is made from a modified SPCE.…”

Copper-Guanosine Nanorods (Cu-Guo NRs) as a Laccase Mimicking Nanozyme for Colorimetric Detection of Rutin

“…Various analytical methods have been introduced so far for Rtn determination, including high-performance liquid chromatography (HPLC) [ 14 ], capillary electrophoresis [ 15 ], chemiluminescence [ 16 ], flow injection analysis [ 17 ], and electrochemistry [ 18 ]. Despite the accuracy of some of them, these methods are mainly limited due to being costly, time-consuming, or requiring tedious sampling equipment, sophisticated instrumentation, and trained technicians [ 1 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 ]. Therefore, developing a rapid, easy-to-use, and cost-effective sensor for on-site monitoring of Rtn is highly desirable.…”