Antimicrobial activity and bioautographic study of antistaphylococcal components from Caesalpinia pyramidalis Tull.

Abstract

The antimicrobial activity of dry methanol and ethyl acetate extracts for the leaves, bark of the stem, peel of the root, flower, fruit and seed of Caesalpinia pyramidalis Tull. (catingueira) was performed against seventeen isolates of Staphylococcus aureus MRSA multiresistant strains, which included two isolates of S. aureus MSSA and two ATCC strains. The antimicrobial activity was tested by the agar diffusion method and the Minimum Inhibitory Concentration (MIC) was determined. The dry methanol extract of the root showed good antimicrobial activity with a MIC of less than 0.5 mg.mL . The dry ethyl acetate extracts exhibited lower antimicrobial activity, which might be explained by solubility problems and less diffusion in the agar medium. Results of the bioautographies also confirmed inhibition halos corresponding to the active substances present in the leaves, as well as in the flower of C. pyramidalis. The phytochemical study of the leaves, bark of the stem, peel of the root, flower and fruit of extracts from C. pyramidalis confirmed the presence of a number of known antimicrobial agents including ursolic acid, quercetin, catechin, ellagic acid, sitosterol, flavonoids, proanthocyanidins and gallic acid. A determinação da atividade antimicrobiana dos extratos metanólicos e em acetato de etila da folha, casca do caule, casca da raiz, flor, fruto e semente de Caesalpinia pyramidalis Tull. foi realizada frente a dezessete isolados de Staphylococcus aureus MRSA multirresistentes, dois isolados de S. aureus MSSA e duas cepas padrão, pelas técnicas de poço/difusão em ágar e determinação das CMI pelo método de diluição em agar/multiinoculador de Stears. O extrato metanólico de casca da raiz indicou uma boa atividade, com CMI inferior a 0.5 mg.mL -1 . Os extratos secos por extração em acetato de etila apresentaram menor atividade que se poderia explicar por problemas de solubilidade e menor difusão no meio de cultura em ágar. Resultados das bioautografias confirmaram zonas de inibição correspondente às substâncias ativas presente na folha, como também na flor da C. pyramidalis. No estudo fitoquímico das folhas, casca da caule, casca da raiz, flor e fruto dos extratos de C. pyramidalis evidenciou-se a presença de vários constituintes com reconhecida atividade antimicrobiana, entre estes o ácido ursólico, quercetina, catequina, ácido elágico, sitosterol, flavonóides, proantocianidinas e ácido gálico. Entre todos os metabólitos citados, somente o ultimo não observamos, por CCD, na casca da raiz de C. pyramidalis. Unitermos

Evaluation of phenolic-linked bioactives of camu-camu ( Myrciaria dubia Mc. Vaugh) for antihyperglycemia, antihypertension, antimicrobial properties and cellular rejuvenation

“…In chronic disease, immunity is always affected and increased susceptibility to bacterial infections and antimicrobial activity can be a common problem. Antimicrobial activity related to phenolic compounds, such as tannic acid, quercetin, catechin, ellagic acid, proanthocyanidins and gallic acid, have been reported earlier (Kil et al, 2009;Rauha et al, 2000;Saraiva et al, 2012). Among all studied pathogenic microorganisms, only growth of S. aureus ATCC 29213 was inhibited by the crude extracts of camu-camu powders (Table 3).…”

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Evaluation of the antiplasmodial and leishmanicidal potential of Myrciaria dubia (Myrtaceae) extract

“…Saraiva et al (28) observed that the Caesalpinia pyramidalis Tull extract showed anti-staphylococcal activity attributable to the presence of quercetin, catechins, ellagic acid, flavonoids, proanthocyanidins, and gallic acid. Rauha et al (29) showed that pure compounds such as flavone, quercetin, naringenin, morin, and kaempferol inhibited Staphylococcus aureus.…”

Traditional use, phytochemistry and biological activities of Poincianella pyramidalys (Tul.) LP Queiroz

“…In their study, Saraiva et al (2012a) Lima, 1996;Agra et al, 2007a;2007b;Cartaxo et al, 2010;Albuquerque et al, 2007;Albuquerque et al, 2006;Oliveira et al, 2010;Marinho et al, 2011;Silva and Albuquerque, 2005 ) was observed. The antimicrobial efficacy of these extracts against Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae, Salmonella spp., S. aureus and Pseudomonas aeruginosa (Saraiva et al, 2012b) was observed.…”