Evaluation of phenolic-linked bioactives of camu-camu ( Myrciaria dubia Mc. Vaugh) for antihyperglycemia, antihypertension, antimicrobial properties and cellular rejuvenation

Abstract

Camu-camu (Myrciaria dubia (Kunth) Mc. Vaugh (Myrtaceae)) has shown potential for food and human health applications due to its rich bioactive functional properties linked to high antioxidant activity. The main objective of this study was to identify antioxidant-linked bioactive phenolic profiles in spray-dried and freeze dried camucamu pulp and to evaluate potential functionality associated with anti-hyperglycemia and anti-hypertension properties using in vitro enzyme assay models. Further, antimicrobial properties and planarian-based cellular protection and regeneration were also investigated. Phenolic compounds, such as ellagitannins, ellagic acid, quercetin glycosides, syringic acid and myricetin were detected in camu-camu by LC-TOF-MS. Freeze dried camucamu powder showed superior phenolic-linked antimicrobial properties and higher hyperglycemia relevant enzyme inhibitory activity compared to the spray dried samples. Besides that, freeze-dried powders and spraydried (6% gum arabic at 120°C) powders were more effective against prokaryotic Staphylococcus aureus and showed higher inhibition than ampicillin. Cellular regeneration of eukaryotic planarian model was stimulated with camu-camu powders when compared to control and showed potential of camu camu for redox-linked cellular protection and rejuvenation. Overall camu-camu rich in phenolic bioactive profiles showed superior antidiabetic and antimicrobial properties and has potential as part of dietary strategies in the management of early stages of type 2 diabetes and associated complications.

From byproduct to a functional ingredient: Camu-camu (Myrciaria dubia) seed extract as an antioxidant agent in a yogurt model

“…These values were higher than those in camu-camu powder produced by spouted bed drying (8.16 g of GAE/100 g and 7.22 g of quebracho tannin equivalent/100 g; Fujita et al, 2013). Several phenolic acids, flavonoids, ellagitannins, and trans-resveratrol have already been identified in different parts of the camu-camu pulp, peel, or seed coat (Genovese et al, 2008;Chirinos et al, 2010;Rufino et al, 2010;Fracassetti et al, 2013;de Azevêdo et al, 2014;Fujita et al, 2015;Kaneshima et al, 2016). In fact, Fujita et al (2015) identified quercetin derivatives; ellagic, syringic, and gallic acids; myricetin; cyanidin-3-o-glucoside; castalagin; and vescalagin in camu-camu powder (peel and pulp).…”

“…Several phenolic acids, flavonoids, ellagitannins, and trans-resveratrol have already been identified in different parts of the camu-camu pulp, peel, or seed coat (Genovese et al, 2008;Chirinos et al, 2010;Rufino et al, 2010;Fracassetti et al, 2013;de Azevêdo et al, 2014;Fujita et al, 2015;Kaneshima et al, 2016). In fact, Fujita et al (2015) identified quercetin derivatives; ellagic, syringic, and gallic acids; myricetin; cyanidin-3-o-glucoside; castalagin; and vescalagin in camu-camu powder (peel and pulp). Fracassetti et al (2013) also identified gallocatechin derivatives, procyanidin dimer B type, (+)-catechin, catechin gallate, and other glycosylated ellagic acid derivatives.…”

“…However, its peel and seeds are discarded by frozen pulp companies, generating a large quantity of byproduct. The consumption of camu-camu pulp is related to its unique taste and high ascorbic acid content, but recently chemical and functional analyses of camu-camu byproducts have indicated in vitro and in vivo functional properties, such as antioxidant, antihyperglycemic, antihyperlipidemic, antihypertensive, antimicrobial, and neuroprotective effects (de Souza Schmidt Gonçalves et al, 2014;Azevêdo et al, 2015;Fujita et al, 2015;Miyashita et al, 2017;Fidelis et al, 2018). Camu-camu byproducts have the potential to be used in developing new bioactive-rich foods and possibly eliminating the use of synthetic chemical preservatives in processed foods.…”

Phytobioactive compound-based nanodelivery systems for the treatment of type 2 diabetes mellitus – current status

“…[109][110][111][112][113] Although quercetin shows beneficial activities in various cell and animal antidiabetes study models, its efficacy through oral delivery systems is very low due to the postabsorptive metabolism and gastrointestinal conditions. [114][115][116][117] Several approaches have been carried out to improve the bioavailability of quercetin in order to improve its bioactivity.…”

Efeito dos compostos fenólicos do camu-camu e do cupuaçu no desenvolvimento da obesidade e diabetes mellitus tipo 2

“…Camu camu aqueous extracts were also reported as highly effective in inhibiting α-glucosidase activity (Fujita et al, 2015), and some polyphenols found in camu camu, including quercetin, myricetin, ellagitannins and EGCG, have been demonstrated to inhibit the sodium dependent glucose transporter and GLUT 2 in vitro . However, as we analyzed glucose tolerance intraperitoneally, it is not clear if these mechanisms of decreasing intestinal carbohydrate absorption could be related to the improvement of glucose homeostasis observed.…”