Antioxidant activity and physical-chemical properties of spray and spouted bed dried extracts of Bauhinia forficata

Abstract

Two distinct drying methods (spouted bed and spray drying) were used for production of dried extracts of Bauhinia forficata Link (Leguminosae, Caesalpinoideae). High-quality powder products in terms of physical and chemical properties were obtained. HPLC fingerprints revealed that the chromatographic profiles of flavonoid compounds present in the dried extract did not change significantly, due to drying. In general, the spouted bed drying caused a degradation of total flavonoids than was lower than that of the spray drying. Antioxidant properties of the dried extract, examined by their radical scavenging activity using 2,2-diphenyl-1-picrylhydrazyl radical (DPPH · ) and inhibition of lipid peroxidation induced by Fe +2 assays (LPO), confirmed their antioxidant potential. The slight reduction in scavenging activity of the dried extracts may be associated with the occurrence of oxidative reactions, decomposition or losses of thermolabile compounds, induced by the heat.Uniterms: Bauhinia forficata/antioxidant activity. Bauhinia forficata/dried extract/properties. Flavonoids/ qualitative analysis. Spouted bed/utilization. Spray drying/utilization.Neste trabalho foram utilizados dois distintos secadores (leito de jorro e spray dryer) para a produção de extratos secos de Bauhinia forficata Link (Leguminosae, Caesalpinoideae) obtendo-se um produto seco com elevada qualidade em termos de suas propriedades físicas e químicas. Análises qualitativas obtidas por CLAE revelam que os perfis cromatográficos dos compostos flavonóides presentes nos extratos secos não apresentaram significativas mudanças durante a secagem quando comparados aos perfis obtidos para os extratos concentrados. Em geral, a secagem por leito de jorro acarreta menores perdas dos flavonóides totais do que a secagem em spray drying. A atividade antioxidante dos extratos secos foi examinada pelos métodos de (DPPH · ) e peroxidação lipídica induzida por Fe +2 (LPO). Uma pequena redução na atividade sequestradora de radicais livres observada para os extratos secos pode ser associada com a ocorrência de reações oxidativas, decomposição e/ou perdas de compostos termolábeis induzidas pelo calor.Unitermos: Bauhinia forficata/atividade antioxidante. Bauhinia forficata/extrato seco/propriedades. Flavonóides/análise qualitativa. Leito de jorro/utilização. Spray drying/utilização.

Plants used as antidiabetics in popular medicine in Rio Grande do Sul, southern Brazil

“…Popular names such as "insulin" and "vegetal insulin" were repeatedly mentioned, for four species of two different families: Aspilia montevidensis and Sphagneticola trilobata (Asteraceae); and Tea, flower dust Garlet and Irgang (2001) Antihyperglycemic activity of the extract by simulation of secretion via pancreatic islets (Hsu et al, 2009); butanol fraction showed potential to prevent Th1-mediated diabetes but promoted Th2-mediated inflammation (Chang et al, 2004(Chang et al, , 2005; water ethanol extracts reduced the hyperglycemia in mildly diabetic mice (Alarcon-Aguilar et al, 2002); The aqueous alcohol extract of the aerial parts showed hypoglicemic activity attributed to polyacetylenic glucosides (Ubillas et al, 2000) Whole (2001) Antioxidant activity related to antidiabetic activity (Souza et al, 2009;Khalil et al, 2008); kaempferol-3-neohesperidoside showed mimetic effect like insulin (Cazarolli et al, 2009b); absence of antidiabetic activity (Volpato et al, 2008); antidiabetic effect attributed to kaempferitrin (Jorge et al, 2004); for review (Cechinel, 2009) (Schoenfelder et al, 2010); aqueous leaf extracts showed hypoglicemic activity in hyperglycemic patients (Bopp et al, 2009); hypoglicemic activity attributed to cuminoside from seeds (Farswan et al, 2009); hypoglycemic activity of mycaminose extracted from seed (Kumar et al, 2008); ethanolic extract of seeds showed hypoglicemic activity in rats (Singh and Gupta, 2007) absence of antihyperglycemic effect of tea and extracts prepared from leaves in normal rats, rats with streptozotocin-induced diabetes, normal volunteers and patients with diabetes ; in vitro glucose uptake activity (Anandharajan et al, 2006); aqueous extract decreased the blood glucose in normal and diabetic rats (Rafiullah et al, 2006); dried bark extract showed antihyperglycemic and hypoglicemic activities (Villaseñor and Lamadrid, 2006); tea prepared from leaves of S. cumini do not showed hypoglycemic effect in patients with type 2 diabetes mellitus ; extract do not reduced the glycemia in diabetic mice the tea and extracts prepared from leaves are pharmacologically inert in diabetic patients (Teixeira et al, 2004); the treatment with tea prepared from leaves did not produce any antihyperglycemic effect in young volunteers neither the crude extract prepared from leaves in diabetic or non-diabetic rats (T...…”

Physicochemical and sensory characteristics of snack bars added of jerivá flour (Syagrus romanzoffiana)

“…The absorbance was measured at 520 nm in spectrophotometer (Beckman 640B) and the results expressed in mg of ascorbic acidity/100 g snack bar. The antioxidant activity was estimated with the stable free radical 2, 2-diphenyl-1-picryl hydrazyl (DPPH), and the results expressed in percentage of free radical scavenging using the equation proposed by Souza et al (2009).…”

Padronização e avaliação biológica de extratos secos de Psidium guajava L. obtidos por spray drying

“…De acordo com o tipo do solvente utilizado e o método de extração, diferentes tipos de substâncias são extraídos e consequentemente a atividade biológica pode ser alterada (SOUZA;GEORGETTI et al, 2009).…”