Genetic variability among cassava accessions based on SSR markers

Ribeiro, Márcia de Nazaré Oliveira; Carvalho, Samuel Pereira de; Santos, João Bosco dos; Antônio, Rafaela Priscila

doi:10.1590/s1984-70332011000300009

Cited by 12 publications

(8 citation statements)

References 15 publications

(14 reference statements)

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“…The genetic variability of cassava in Brazil is maintained in work collections and germplasm banks, where it is represented mainly by native varieties and/or those selected by farmers (Ribeiro et al, 2011). Another alternative is on-farm conservation, which is performed on small farms where the producers select cassava landraces according to their needs.…”

Section: Introductionmentioning

confidence: 99%

Research Article Genetic diversity of cassava landraces cultivated in northern Mato Grosso State, Brazil, using microsatellite markers

Pedri¹,

Hoogerheide²,

Tiago³

et al. 2019

Genet. Mol. Res.

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Cassava (Manihot esculenta) is a traditional crop in tropical and subtropical regions that is used for human consumption and in animal feed. This studied cassava landraces cultivated in northern Mato Grosso State, Brazil, to detect the variability in family farms, aiming at the preservation and use potential of these genetic resources. A total of 120 M. esculenta individuals were evaluated at the rate of 40 per location (population), where each landrace was represented by 10 plants. The 14 microsatellite markers examined showed genetic diversity. The average polymorphic information content (PIC) was 0.621 and the average number of alleles per locus was seven. Genetic diversity parameters indicated that H o was higher than H e for the three populations, showing negative fixation indices and a lack of inbreeding. AMOVA revealed greater molecular variation within the populations (92%). Bayesian analysis and the ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 18 (3): gmr18315 E.C.M. Pedri et al. 2UPGMA clustering method resulted in two main groups formed with the individuals distributed randomly; i.e., regardless of collection site (location). The gene flow found in this study is a consequence of the introduction and exchange of genetic material (landraces) performed by the farmers, who act on their farms as maintainers of local diversity. Manihot esculenta cultivation in northern Mato Grosso State ensure the on-farm conservation of genetic variability of the species and constitutes a source of genetic resources such as genes of resistance and adaptation, which can be exploited and utilized in breeding programs.

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Section: Introductionmentioning

confidence: 99%

Research Article Genetic diversity of cassava landraces cultivated in northern Mato Grosso State, Brazil, using microsatellite markers

Pedri¹,

Hoogerheide²,

Tiago³

et al. 2019

Genet. Mol. Res.

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“…Although there is much information regarding the genetic diversity of cassava, most of them are about samples from Africa (Mkumbira et al 2003, Moyib et al 2007, from the Southeast of Brazil (Siqueira et al 2010, Ribeiro et al 2011, Costa et al 2013, or from West of Amazonia (Mühlen et al 2000, Elias et al 2004, Siqueira et al 2009, Alves-Pereira et al 2011. Pará is the main producer of cassava in Brazil, and its population maintains old habits of cassava use inherited from the indigenous people, such as the use of cassava leaves and starch to produce regional foods.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of 'sweet' cassavas (Manihot esculenta) from a germplasm bank in Brazilian Eastern Amazonia

Cunha

Silva

Albuquerque

et al. 2016

Crop Breed. Appl. Biotechnol.

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“…The identification of redundancies in collections of vegetatively propagated species is common (IRISH et al, 2010;RIBEIRO et al, 2011;GROSS et al, 2012), probably due to material exchanges between farmers where the genotype can be given another name in the new location. The presence of duplicates accessions represents substantial expenditure in the preservation of cassava germplasm under field conditions.…”

Section: Resultsmentioning

confidence: 99%

Molecular characterization and core collection evaluation of Manihot esculenta Crantz

et al. 2020

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Cassava is one of the most important subsistence crops in tropical regions. It is necessary to preserve and to know the genetic diversity existent for the adequate use of genetic resources. The evaluation of genetic diversity among genotypes results in information about potential parents in breeding programs, allows duplicates identification, and facilitates germplasm exchange between research institutions. The objective of this study was to characterize the genetic diversity of cassava accessions of North Brazil region. A total of 106 accessions were analyzed using ten microsatellite markers. The genetic parameters estimated were: expected heterozygosity (HE), observed heterozygosity (HO) and polymorphic information content (PIC). Clustering was performed using the UPGMA and Neighbor-Joining (NJ) method. Bayesian analysis, analysis of principal coordinates and identification of a core collection were also used. The ten loci amplified 8,40 alleles on average. The average heterozygosity estimates were: HE = 0.71, HO = 0.58 and PIC = 0.72. Genetic distances ranged from 0.158 to 0.908. Six (5,66%) accesses were redundant. Clustering and dispersion analysis didn’t differentiate bitter from sweet cassava, and there wasn’t correlation between groups and collect origin. The core collection consisted of 22 individuals that represented 94% of total allelic diversity and 20,75% of the base collection. The results indicate high dissimilarity between the accessions and allowed the detection of redundant genotypes, showing the use of genetic markers as informative tools for the management of collections.

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Genetic variability among cassava accessions based on SSR markers

Abstract: ABSTRACT

Cited by 12 publications

References 15 publications

Research Article Genetic diversity of cassava landraces cultivated in northern Mato Grosso State, Brazil, using microsatellite markers

Research Article Genetic diversity of cassava landraces cultivated in northern Mato Grosso State, Brazil, using microsatellite markers

Molecular characterization of 'sweet' cassavas (Manihot esculenta) from a germplasm bank in Brazilian Eastern Amazonia

Molecular characterization and core collection evaluation of Manihot esculenta Crantz

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