Urinary tract infection is characterized by a variety of clinical conditions resulting from the presence of microorganisms in the urinary tract.In a patient with bladder catheter delay, the risk of infection increases significantly, thus requiring the use of antimicrobials. The present study aims to discuss the knowledge of the nurse of the Intensive Care Center in relation to the use of antimicrobial, as well as the importance of scientific knowledge to intervene administratively and assistentially. This is a qualitative, descriptive, exploratory study. Data were collected through a structured questionnaire and secondary source such as medical records. The nursing records of 111 medical records were analyzed and a structured questionnaire was applied to 12 nurses (o). There was a predominance of antimicrobials of the class of Cephaslosporins and Quinolones corresponding respectively to 30.4% and 15.9% of all prescriptions. Regarding antimicrobial indications, 67.0% of professionals reported having knowledge, dosage 56.0% and adverse effects 22.0%. However, in 100.0% of the charts, no records and nursing prescriptions were observed that addressed care regarding adverse effects, drug interactions and microbial resistance. The urgent need for educational intervention in the service and training of professionals to improve planning and assistance was evidenced.