Identification of sources of seedling resistance to Phytophthora capsici in Cucumis melo

Abstract

The employment of genetic resistance to minimize yield losses induced by Phytophthora capsici remains unexplored in melon (Cucumis melo). A diverse collection of melon accessions was evaluated against P. capsici isolates at the seedling stage. In the first screening assay, 105 accessions were evaluated using isolate PCpe-04 obtained from cucumber (Cucumis sativus). In a second assay, 31 accessions displaying high levels of resistance in the first assay were challenged with a distinct isolate (PCpe-09 also from cucumber). In a third assay, a subset of 14 selected accessions was re-evaluated using isolates PCpe-09 and PCmo-07 (from strawberry). In the last screening, seven accessions with high levels of resistance across all assays were challenged with five isolates from representative host species [PC-Vagem (snap bean), PCp-129 (Capsicum chinense), PCp-155 (C. annuum), PCpe-09 and PCmo-07] to assess their reaction against a varied sample of P. capsici isolates. For two accessions (CNPH-093 and L040), all plants remained free of symptoms after inoculation with all five isolates. Accessions WMR-29, CNPH 084, CNPH 088 and CNPH 092 were also free of symptoms to all isolates, except PCmo-07. These large-spectrum resistance sources might be useful for breeding programs aiming to incorporate resistance against P. capsici in elite melon lines.

Transcriptome analysis clarified genes involved in resistance to Phytophthora capsici in melon

“…Melon (Cucumis melo L.) is an economically important fruit worldwide and plays an important role in promoting income of growers, as well as improving the diet structure of local residents. However, the expansion of melon growing areas and its intensive year-round cultivation result in the occurrence of serious biotic challenges, especially soil-borne diseases, which lead to substantial decreases in melon yield and quality [1].…”