Cultural and morphological characterization of Pochonia chlamydosporia and Lecanicillium psalliotae isolated from Meloidogyne mayaguensis eggs in Brazil

Arévalo, Jersys; Hidalgo-Díaz, L; Martins, Irene; Souza, Janaína F; Castro, J. M. C.; Carneiro, R. M. D. G.; Tigano, Myrian S.

doi:10.1590/s1982-56762009000300004

Cited by 23 publications

(8 citation statements)

References 13 publications

(10 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Also, these results agree with the findings of Maity and Samaddar (1977) Zhang et al (1996) and Shabana et al (2000) using different fungal agents. Nagesh et al (2007) obtained similar findings, whereas they found that the best temperature for growth of P. chlamydosporia was 25-35 o C. Also, our results are in line with those of Arevalo et al (2009) who reported that the optimum temperature for growth and spore production for P. chlamydosporia isolates ranged between 24 and 28°C and those of Duponnois et al (1995) who observed that optimum growth of A. oligospora occurred at 25-30°C. Also, Nagesh et al (2005) demonstrated that the optimum temperature for A. oligospora growth, sporulation, conidiospore germination and conidiospore production ranged between 25 and 35°C.…”

Section: Discussionsupporting

confidence: 91%

EFFECT OF NUTRITION AND PHYSICAL FACTORS ON MYCELIAL GROWTH AND SPORE PRODUCTION OF Curvularia prasadii, A MYCOHERBICIDE AGENT FOR BARNYARD GRASS (Echinochloa crus-galli) IN RICE

Shabana¹,

AbouTabl²,

Sadek³

2015

Journal of Plant Protection and Pathology

View full text Add to dashboard Cite

Echinochloa crus-galli is an important weed in rice paddies. The optimum cultural and physical conditions for production of Curvularia prasadii (CP01, CP02, and CP03 isolates), a biocontrol agent for the rice weed, E. crus-galli, were determined. Culture media, light regime, pH, incubation temperature, and aeration were tested for their effect on the mycelial growth and spore production of the fungal biocontrol agent. Showed to be the best medium for the linear growth of the three isolates of the fungus was malt extract agar (MEA). Maximum yield of spores of the three isolates was obtained on potato dextrose agar (PDA). Contentious darkness was the most suitable light regime for the mycelial growth and sporulation of the CP01 and CP02 isolates, while contentious or diurnal light were best for CP03 growth. However, all degrees light regime were sutable for the spore production of the isolate CP03, with no significant differences among them. The pH levels of 6, 7, and 8 induced the highest mycelial growth of the three isolates. However, the latter two pH levels (7 & 8) were best for the sporulation of all three isolate. The best mycelial growth was obtained at incubation temperature of 30°C (for CP01 and CP03) and 35°C (for CP02). For spore production, the optimum level of temperature for isolates CP01 and CP02 was 35°C, while it was 30°C for CP03. Aeration level (as negatively correlated with the number of Parafilm ® layers around the culture plate edge) was also tested. There was no effect of the number of Parafilm ® layers (aeration level) on the CP02 mycelial growth. However, two layers of sealing culture plates with Parafilm ® provided optimum level of aeration for best mycelial growth for isolates CP02 and CP03 and highest spore production for all three isolates.

show abstract

Section: Discussionsupporting

confidence: 91%

EFFECT OF NUTRITION AND PHYSICAL FACTORS ON MYCELIAL GROWTH AND SPORE PRODUCTION OF Curvularia prasadii, A MYCOHERBICIDE AGENT FOR BARNYARD GRASS (Echinochloa crus-galli) IN RICE

Shabana¹,

AbouTabl²,

Sadek³

2015

Journal of Plant Protection and Pathology

View full text Add to dashboard Cite

show abstract

“…The egg-parasitic fungi Pochonia chlamydosporia (formerly Verticillium chlamydosporium) and Purpureocillium lilacinum (formerly Paecilomyces lilacinus) are among the most studied biological agents aiming at nematode management (Hidalgo-D ıaz et al, 2000;Atkins et al, 2003;Khan et al, 2006;Anastasiades et al, 2008;Moosavi et al, 2010;Carneiro et al, 2011). Both species are ubiquitous, saprobic filamentous fungi, commonly isolated from soils, root surfaces, and some invertebrates, being naturally distributed in agricultural soils across Brazil (Tigano-Milani et al, 1993;Arevalo et al, 2009). Characteristics such as their ability to successfully colonize the rhizosphere and mass production feasibility (De Leij and Kerry, 1991;Kerry and Hidalgo-D ıaz, 2004;Rumbos and Kiewnick, 2006) make these species potential candidates for the development of bionematicides.…”

mentioning

confidence: 99%

Evaluation of Pochonia chlamydosporia and Purpureocillium lilacinum for Suppression of Meloidogyne enterolobii on Tomato and Banana

Silva

Carneiro

Faria

et al. 2017

Journal of Nematology

View full text Add to dashboard Cite

Meloidogyne enterolobii is one of the most important root-knot nematode in tropical regions, due to its ability to overcome resistance mechanisms of a number of host plants. The lack of new and safe active ingredients against this nematode has restricted control alternatives for growers. Egg-parasitic fungi have been considered as potential candidates for the development of bionematicides. In tissue culture plates, Pochonia chlamydosporia (var. catenulata and chlamydosporia) and Purpureocillium lilacinum strains were screened for their ability to infect eggs of the root-knot nematode M. enterolobii on water-agar surfaces. Reduction in the hatching of J2 varied from 13% to 84%, depending on strain. The more efficacious strains reduced hatchability of J2 by 57% to 84% when compared to untreated eggs, but average reductions were only 37% to 55% when the same strains were applied to egg masses. Combinations of fungal isolates (one of each species) did not increase the control efficacy in vitro. In experiments in which 10,000 nematode eggs were inoculated per plant, reductions in the number of eggs after 12 months were seen in three of four treatments in banana plants, reaching 34% for P. chlamydosporia var. catenulata. No significant reductions were seen in tomato plants after 3 mon. In another experiment with tomato plants using either P. chlamydosporia var. catenulata or P. lilacinum, the number of eggs was reduced by 34% and 44%, respectively, when initial infestation level was low (500 nematode eggs per plant), but tested strains were not effective under a moderate infestation level (5,000 eggs per plant). Under all infestation levels tested in this work, gall and egg mass indexes (MI) did not differ from the untreated controls, bringing concerns related to the practical adoption of this control strategy by farmers. In our opinion, if the fungi P. chlamydosporia and P. lilacinum are to be used as biocontrol tools toward M. entorolobii, they should focus on agricultural settings with low soil infestation levels and within an IPM approach.

show abstract

“…Most Trichoderma strains are mesophilic, and cannot protect germinating seeds from soilborne diseases caused by cold tolerant strains of plant pathogenic fungi during cold autumn and spring conditions. The mycelial growth and sporulation of P. chlamydosporia are influenced by components of the medium and culture conditions (Kerry et al1986;Zaki et al 1993) (Nagesh et al 2007) report that best temperature for growth of P. chlamydosporia was 25-35 o C. (Arevalo et al 2009) report that the optimum temperature for growth and spore production ranged between 24 and 28°C for P. chlamydosporia isolates. (Fernandes et al 199) all fungal isolates grew faster at a constant 20°C on Potato Dextrose Broth.…”

Section: Discussionmentioning

confidence: 99%

Effect of cultural condition on biomass production of some Nematophagous fungi as biological control agent

Jokar

Zarabi

Hasanzadeh

et al. 2012

Egyptian Academic Journal of Biological Sciences. A, Entomology

View full text Add to dashboard Cite

Nematophagous fungi include a group of fungal antagonists to nematodes. The nematophagous fungi have been suggested as promising candidates for biological control of plant parasitic nematodes. Impacts of different pH levels, temperature, light intensity, carbon and nitrogen source were tested on the growth of five nematophagus fungi under in vitro conditions. Three isolates of Trichoderma harzianum T7, T8, and T14, one isolate of Pochonia chlamydosporia var. chlamydosporia and one isolate of Arthrobotrys oligospora were tested. The results of experiment indicated that the growth of isolates of Trichoderma harzianum was maximum in pH range of 7.00-8.00. The best growth of Pochonia chlamydosporia var. chlamydosporia and Arthrobotrys oligospora was in 4.00-7.00. Effect of temperature differs between range of 15-30°C, for isolates of Trichoderma harzianum was 20-25°C, Pochonia chlamydosporia var. chlamydosporia was 15-20°C, and Arthrobotrys oligospora was 20-25°C. Maximum growth of two isolates of Trichoderma harzianum T7, T8 and Pochonia chlamydosporia var. chlamydosporia was in darkness. Best growth of one isolates of T. harzianum (T7) was in light and maximum growth of Arthrobotrys oligospora and Trichoderma harzianum T14 occurred in 12 h light and 12h dark. The best source of nitrogen for Trichoderma harzianum (T8,T14) was Nitrate potassium, while Nitrate ammonium was the best nitrogen source for (T7).The maximum growth of A. oligospora and P. chlamydosporia occuerd in Nitrate sodium. Between carbon sources Arabinose are suitable for A.oligospora and Trichoderma harzianum (T7).The maximum growth of P. chlamydosporia and T. harzianim (T14) occuerd in the use of fructose. Best growth of T. harzianum (T8) was in Mnitole.

show abstract

Cultural and morphological characterization of Pochonia chlamydosporia and Lecanicillium psalliotae isolated from Meloidogyne mayaguensis eggs in Brazil

Cited by 23 publications

References 13 publications

EFFECT OF NUTRITION AND PHYSICAL FACTORS ON MYCELIAL GROWTH AND SPORE PRODUCTION OF Curvularia prasadii, A MYCOHERBICIDE AGENT FOR BARNYARD GRASS (Echinochloa crus-galli) IN RICE

EFFECT OF NUTRITION AND PHYSICAL FACTORS ON MYCELIAL GROWTH AND SPORE PRODUCTION OF Curvularia prasadii, A MYCOHERBICIDE AGENT FOR BARNYARD GRASS (Echinochloa crus-galli) IN RICE

Evaluation of Pochonia chlamydosporia and Purpureocillium lilacinum for Suppression of Meloidogyne enterolobii on Tomato and Banana

Effect of cultural condition on biomass production of some Nematophagous fungi as biological control agent

Contact Info

Product

Resources

About