Chrysin reduces proliferation and induces apoptosis in the human prostate cancer cell line pc-3

Abstract

INTRODUCTION:Honey is a common household product with many medicinal uses described in traditional medicine. Only recently has its antioxidant properties and preventive effects against disease been highlighted. Chrysin is a natural flavone commonly found in honey that has been shown to be an antioxidant agent. In this study, we investigated the antiproliferative and apoptotic effects of honey and chrysin on cultured human prostate cancer cells.METHODS:Cells were cultured in RPMI medium and treated with different concentrations of honey and chrysin for three consecutive days. Cell viability was quantitated by the 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. The percentage of apoptotic cells was determined by flow cytometry using Annexin V-fluorescein isothiocyanate.RESULTS:The MTT assay revealed that both compounds had an antiproliferative effect on PC-3 cells in a dose- and time-dependent manner. The IC50 values for honey and chrysin against PC-3 cells were 2.5% and 24.5% after 48 h and 1.8% and 8.5% after 72 h, respectively. Chrysin induced apoptosis in PC-3 cells, as determined by flow cytometry.CONCLUSION:Our results suggest that honey has anti-proliferative effects on prostate cancer cells and the effects are mainly due to chrysin. Therefore, chrysin may be a potential compound for both cancer prevention and treatment. Further in vivo investigation is needed to support the use of chrysin in cancer therapy.

Chrysin induces cell apoptosis in human uveal melanoma cells via intrinsic apoptosis

“…For the cells treated with chrysin, cells stained with DAB (indicating apoptotic changes) were significantly increased compared to the controls. This is consistent with previous studies, which have reported an apoptosis-induction effect of chrysin in prostate cancer, lung cancer, leukemia and cutaneous melanoma cells (4,5,9,12).…”

“…Chrysin has been demonstrated to possess cancer chemopreventive activity through inhibition of cell proliferation (10,18), and induce apoptosis in various malignant cancer cells (5,11,(15)(16)(17)(20)(21)(22).…”

“…1A and B), and the presence of at least two hydroxyl groups in positions 3, 5 and 7 of the A ring is reported to be necessary to confer pro-apoptotic activity (4). Chrysin is present in honey, propolis, and other plants and herbs (5). Chrysin was initially identified for its anti-oxidant effects and has been shown to possess cancer chemopreventive and anti-tumor effects (6)(7)(8).…”

“…Chrysin was initially identified for its anti-oxidant effects and has been shown to possess cancer chemopreventive and anti-tumor effects (6)(7)(8). It inhibits proliferation and induces apoptosis in a variety of cancer cells in vitro, including cells from the prostate, skin, breast, lung, cervix, thyroid cancer and leukemia (5,(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22). However, to the best of our knowledge, the effects of chrysin on uveal melanoma cells have not been previously studied.…”

Development of chrysin loaded poloxamer micelles and toxicity evaluation in fish embryos

“…Chrysin, a natural flavonoid compound with the IUPAC name 5,7-dihydroxy-2-phenyl-4H-chromen-4-one, can be extracted from plants, honey, and propolis (1). It has been shown that chrysin is abundant in the fruit of Oroxylum indicum (2).…”