The antimicrobial effect of 0.1 ppm ozonated water on 24-hour plaque microorganisms in situ

Syed, Sadatullah; Mohamed, Nor Himazian Binti; Razak, Fathilah Abdul

doi:10.1590/s1806-83242012000200007

Cited by 29 publications

(24 citation statements)

References 25 publications

(21 reference statements)

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“…Different concentrations of ozone solutions were tested for eradication of different microorganisms in literatures 8212223. Generally, as the concentration of ozone increases, the antimicrobial efficacy increases as well.…”

Section: Discussionmentioning

confidence: 99%

Influence of ozone and paracetic acid disinfection on adhesion of resilient liners to acrylic resin

Ekren

Özkömür

2016

J Adv Prosthodont

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PURPOSEThe aim of this study was to evaluate the effect of paracetic acid (PAA) and ozone disinfection on the tensile bond strength (TBS) of silicone-based resilient liners to acrylic resins.MATERIALS AND METHODSOne hundred and twenty dumbbell shaped heat-polymerized acrylic resins were prepared. From the mid segment of the specimens, 3 mm of acrylic were grinded off and separated parts were reattached by resilient liners. The specimens were divided into 2 control (control1, control7) and 4 test groups of PAA and ozone disinfection (PAA1, PAA7, ozone1 and ozone7; n=10). While control groups were immersed in distilled water for 10 min (control1) and 7 days (control7), test groups were subjected to PAA (16 g/L) or ozone rich water (4 mg/L) for 1 cycle (10 min for PAA and 60 min for ozone) per day for 7 days prior to tensile tests. Measurements of the TBS were analyzed using 3-way ANOVA and Tukey's HSD test.RESULTSAdhesive strength of Mollosil decreased significantly by application of ozone disinfection. PAA disinfection had no negative effect on the TBS values of Mollosil and Molloplast B to acrylic resin. Single application of ozone disinfection did not have any negative effect on TBS values of Molloplast B, but prolonged exposure to ozone decreased its adhesive strength.CONCLUSIONThe adhesion of resilient liners to acrylic was not adversely affected by PAA disinfection. Immersion in ozonated water significantly decreased TBS of Mollosil. Prolonged exposure to ozone negatively affects adhesion of Molloplast B to denture base materials.

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Section: Discussionmentioning

confidence: 99%

Influence of ozone and paracetic acid disinfection on adhesion of resilient liners to acrylic resin

Ekren

Özkömür

2016

J Adv Prosthodont

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“…They found that anti‐biofilm activity was increased with increasing ozone concentrations (0·25, 0·5 and 1·0 mg/l, all greatly exceeding the present concentration), although complete eradication of biofilm was not achieved in their 60 second exposure time. In a rare human trial of the anti‐biofilm activity of ozonated water, Sadatullah et al showed that ozonated water at a concentration of 0·1 mg/l resulted in a reduction in bacterial load in plaque , although complete eradication was again not achieved.…”

Section: Discussionmentioning

confidence: 99%

Ozonated saline shows activity against planktonic and biofilm growing Staphylococcus aureus in vitro: a potential irrigant for infected wounds

Al-Saadi

Potapova

Rochford

et al. 2015

International Wound Journal

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Infections associated with deep wounds require extensive surgical and medical care. New adjunctive treatments are required to aid in the eradication of the bacterial biofilms found on infected wounds and, in particular, any underlying hardware. Ozone has been used as a safe and efficient disinfectant in water treatment plants for many years. The purpose of this study is to investigate the anti-biofilm potential of ozonated saline against biofilms of Staphylococcus aureus, a microorganism commonly implicated in wound infections. A custom-made bacterial biofilm bioreactor was used to grow S. aureus biofilms on discs of medical grade titanium alloy. An ozone generator was connected in-line and biofilms and planktonic bacteria were exposed to ozone in saline. Cytotoxicity was assessed against primary ovine osteoblasts in the same system. In tests against planktonic S. aureus, a 99% reduction in bacterial numbers was detected within 15 minutes of exposure. S. aureus biofilms were significantly more resistant to ozone, although complete eradication of the biofilm was eventually achieved within 5 hours. Ozonated saline was not found to be cytotoxic to primary ovine osteoblasts. Ozonated saline may be suitable as an adjuvant therapy to treat patients as an instillation fluid for wound irrigation and sterilisation.

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“…[22] The average reduction observed for the streptococci load (56.4%) was more than that observed for the total bacteria (45.3%) and the anaerobes (51.7%).…”

Section: Discussionmentioning

confidence: 99%

Qualitative analyses of the antimicrobial effect of ozonated water on supragingival plaque and salivary microbes

Syed¹,

Mohamed²,

Razak³

2014

Ann Med Health Sci Res

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Background:Ozone is an unstable gas, capable of oxidizing any biological entity. It is an effective bactericide in its gaseous as well as aqueous form.Aims:The objective of this study was to determine the in-situ antimicrobial effect of 0.1 ppm ozonated water on plaque and salivary microorganisms.Subjects and Methods:24 h old supragingival plaque (SP) was collected from the two most posterior teeth in the contralateral quadrants before and after a 30 s rinse with either distilled water (control group) or 0.1 ppm ozonated water (test group). Simultaneously stimulated whole saliva (SWS) was collected for both groups. The SP and SWS were stained with LIVE/DEAD Baclight Bacteria Viability fluorescent kit to visualize live and dead microbes. The salivary flow rate, calcium and protein level were also determined in the pre- and post-rinsed samples. The data obtained was subjected to One Way ANOVA test using Minitab 14 statistical software (PA, USA).Results:0.1 ppm ozonated water was found effective in reducing the bacterial load in both the 24 h plaque and SWS samples, but it did not eliminate them completely. In addition, there was no statistically significant effect of the ozonated water rinse on the salivary flow rate, salivary calcium and protein concentration.Conclusions:0.1 ppm ozonated water rinse can be an effective adjunct to tooth brushing and flossing to maintain plaque and salivary bacterial load.

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The antimicrobial effect of 0.1 ppm ozonated water on 24-hour plaque microorganisms in situ

Cited by 29 publications

References 25 publications

Influence of ozone and paracetic acid disinfection on adhesion of resilient liners to acrylic resin

Influence of ozone and paracetic acid disinfection on adhesion of resilient liners to acrylic resin

Ozonated saline shows activity against planktonic and biofilm growing Staphylococcus aureus in vitro: a potential irrigant for infected wounds

Qualitative analyses of the antimicrobial effect of ozonated water on supragingival plaque and salivary microbes

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