Qualitative analyses of the antimicrobial effect of ozonated water on supragingival plaque and salivary microbes

Abstract

Background:Ozone is an unstable gas, capable of oxidizing any biological entity. It is an effective bactericide in its gaseous as well as aqueous form.Aims:The objective of this study was to determine the in-situ antimicrobial effect of 0.1 ppm ozonated water on plaque and salivary microorganisms.Subjects and Methods:24 h old supragingival plaque (SP) was collected from the two most posterior teeth in the contralateral quadrants before and after a 30 s rinse with either distilled water (control group) or 0.1 ppm ozonated water (test group). Simultaneously stimulated whole saliva (SWS) was collected for both groups. The SP and SWS were stained with LIVE/DEAD Baclight Bacteria Viability fluorescent kit to visualize live and dead microbes. The salivary flow rate, calcium and protein level were also determined in the pre- and post-rinsed samples. The data obtained was subjected to One Way ANOVA test using Minitab 14 statistical software (PA, USA).Results:0.1 ppm ozonated water was found effective in reducing the bacterial load in both the 24 h plaque and SWS samples, but it did not eliminate them completely. In addition, there was no statistically significant effect of the ozonated water rinse on the salivary flow rate, salivary calcium and protein concentration.Conclusions:0.1 ppm ozonated water rinse can be an effective adjunct to tooth brushing and flossing to maintain plaque and salivary bacterial load.

Treatment of symptomatic, deep, almost cariously exposed lesions using ozone

“…Besides, the findings of this experiment demonstrated that ozone treatment of deep caries was associated with less need for RCT than conventional treatment. This could also be attributed to the fact that ozone is effective for killing cariogenic microorganisms [1][2][3][4][5]8,[12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27]29 . Also, ozone prevents formation of bacterial biofilms on dentine by changing dentine wettability through oxidation of organic elements (such as proteins, carbohydrates, glycolipids and acids) 8,12,17,30,31 .…”

“…Caries management using ozone is conservative, consumes less treatment time, and requires shorter periods of mouth opening 11 . In addition, ozone is efficient in killing and reducing different cariogenic microorganisms (such as streptococcus mutans, streptococcus sobrinus and lactobacilli) in both clinical and laboratory settings, especially using the healozone [1][2][3][4][5]8,[12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31] .Previous studies established that ozone treatment using the healozone could enhance the remineralization process and thus improve the hardness of open single surface shallow carious lesions 4,7,28 . However, significantly less cariogenic microorganisms and more arrested caries were associated with ozone application into small shallow carious lesions but not in very deep large ones 2 .…”

“…Caries management using ozone is conservative, consumes less treatment time, and requires shorter periods of mouth opening 11 . In addition, ozone is efficient in killing and reducing different cariogenic microorganisms (such as streptococcus mutans, streptococcus sobrinus and lactobacilli) in both clinical and laboratory settings, especially using the healozone [1][2][3][4][5]8,[12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31] .…”

Ozone therapy in dentistry: clinical experiences

“…Using hyper-ozonated solutions to cool surgical handpieces, piezo-electric and ablator handpieces, a more effective debridement of the bacterial biofilm 1 with a sharp reduction in the bacterial load of the surgical site. 3 In addition, there is a significant improvement in patient compliance and a much faster healing ( Figure 6). …”

“…After extraction, curettage of the region is carried out, an abundant wash with hyper-ozonated water. 3 The surgical wound is sprinkled with ozonized paste. The surrounding tissues are infiltrated with low concentration ozone in order to promote restorative processes and blood supply.…”