Comparative analysis of human and bovine teeth: radiographic density

Abstract

Since bovine teeth have been used as substitutes for human teeth in in vitro dental studies, the aim of this study was to compare the radiographic density of bovine teeth with that of human teeth to evaluate their usability for radiographic studies. Thirty bovine and twenty human teeth were cut transversally in 1 millimeter-thick slices. The slices were X-rayed using a digital radiographic system and an intraoral X-ray machine at 65 kVp and 7 mA. The exposure time (0.08 s) and the target-sensor distance (40 cm) were standardized for all the radiographs. The radiographic densities of the enamel, coronal dentin and radicular dentin of each slice were obtained separately using the 'histogram' tool of Adobe Photoshop 7.0 software. The mean radiographic densities of the enamel, coronal dentin and radicular dentin were calculated by the arithmetic mean of the slices of each tooth. One-way ANOVA demonstrated statistically significant differences for the densities of bovine and human enamel (p < 0.05) and for bovine and human coronal dentin (p < 0.05). No statistically significant differences were found for the bovine and human radicular dentin (p > 0.05). Based on the results, the authors concluded that: a) the radiographic density of bovine enamel is significantly higher than that of human enamel; b) the radiodensity of bovine coronal dentin is statistically lower than the radiodensity of human coronal dentin; bovine radicular dentin is also less radiodense than human radicular dentin, although this difference was not statistically significant; c) bovine teeth should be used with care in radiographic in vitro studies.

Bovine teeth as substitute for human teeth in dental research: a review of literature

“…Therefore, 68 papers were included in the final review. The studies covered seven main categories: morphology (5 studies) (22)(23)(24)(25)(26), chemical composition (11 studies) (27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37), physical properties (12 studies) (38)(39)(40)(41)(42)(43)(44)(45)(46)(47)(48)(49), dental caries (7 studies) (6,9,(50)(51)(52)(53)(54), dental erosion/abrasion (10 studies) (55)(56)(57)(58)(59)(60)(61)(62)(63)(64), bonding/adhesive strength (17 studies) (65)(66)(67)(68)…”

“…Tanaka et al (47) found that the radiographic density of bovine enamel and coronal dentin was significantly higher than that of human enamel and coronal dentin, respectively. However, no significant difference in the …”

“…Twelve studies were reviewed (38)(39)(40)(41)(42)(43)(44)(45)(46)(47)(48)(49). Putt et al (38) found no significant difference in the polishing degree rank of seven abrasives for human and bovine enamel.…”

Avaliação comparativa da ultraestrutura e propriedades físicas do esmalte bovino, bubalino e humano

“…colhida pela maioria dos pesquisadores, uma vez que são facilmente obtidos e cada vez mais indicados pelos comitês de ética (Seixas et al 2007, Matos et al 2008, Tanaka et al 2008. O Brasil possui o maior rebanho de búfalos das Américas, com a criação em grande escala direcionada para a produção de carne, restando uma alta disponibilidade de material biológico para outros fins, como os estudos científicos (Santana et al 2011).…”

Progressão da lesão de cárie no esmalte dental após aplicação de compostos fluoretados e irradiação com laser de CO2

“…A princípio, a composição química e estrutural do esmalte de todos os mamíferos é igual, porém pode haver alguma variação dependendo da espécie do animal (MORIWAKI et al, 1968). Os dentes bovinos são comumente utilizados em estudos laboratoriais por não possuírem restrições éticas, serem mais facilmente obtidos que os humanos e possuírem tamanho amplo (TANAKA et al, 2008). Estudos demonstraram que apesar de ser mais poroso que o humano (MORIWAKI et al, 1968;MELLBERG, 1992), o dente bovino possui composição mais uniforme (MELLBERG, 1992), podendo produzir resposta aos desafios cariogênicos com menor variação MELLBERG, 1981).…”