Oral candidosis by Candida albicans in normal and xerostomic mice

Abstract

The aim of this study was to analyze the effect of sialoadenectomy on the development of oral candidosis after one or four inoculations of Candida albicans. Initially, a suspension containing 10(8) cells/ml of C. albicans ATCC 36801 was prepared. Seventy-eight sialoadenectomized mice and a similar amount of mice with normal salivary flow received a single inoculation of C. albicans suspension. Another group with a similar number of mice received 4 inoculations. The control group consisted of 6 sialoadenectomized mice and 6 mice with normal salivary flow that were not inoculated with C. albicans. Candidosis development was studied histologically in the tongue of the animals 1, 2, 3, 5, and 8 days after inoculation and at 15-day intervals up to 165 days. According to the results obtained, it could be concluded that sialoadenectomy and a higher frequency of yeast inoculation influenced the presence and extension of candidosis lesions.

Recent mouse and rat methods for the study of experimental oral candidiasis

“…As in rat models, mice models require the use of immunosuppressive agents or other predisposing factors to establish persistent infection since these animals are not naturally colonized by C. albicans 35 , 38 , 46 , 59 - 61 . The effect of xerostomia in sialoadenectomized mice prolonged oral colonization by C. albicans to 75 d post-infection vs. 30 d of colonization for the control animals 38 .…”

“…The effect of xerostomia in sialoadenectomized mice prolonged oral colonization by C. albicans to 75 d post-infection vs. 30 d of colonization for the control animals 38 . With 1 or 4 inoculations with a C. albicans suspension, the last procedure increased the permanence of C. albicans in the oral cavity of sialoadenectomized mice and produced lesions in the tongue that contained pseudohyphae inside the epithelium, acanthosis, and neutrophilic infiltrate forming intraepithelial microabscesses, with a higher prevalence of lesions in the true and simple conic papillae 59 . This model favored the permanence of C. albicans in the oral cavity, and higher frequencies of yeast inoculation influenced the presence and extension of candidiasis lesions.…”

Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis

“…1,2 The predisposing factors of oral candidosis include immunocompromised states, diabetes mellitus, dental prostheses, xerostomia, 3,4 and prolonged use of antibiotics or immunosuppressive drugs. 5,6 With the advent of the human immunodeficiency virus (HIV) infection, increased attention has been paid to oral candidosis, because up to 90% of HIV-infected individuals suffer from oral Candida infection.…”

In vivo evaluation of photodynamic inactivation using Photodithazine® against Candida albicans

“…32 Other methodologies were also proposed to induce oral candidosis in animals. 22,33 Teichert et al 22 used a protocol of severe combined immunodeficiency disease (SCID) to induce oral candidosis and recovered approximately 2 × 10 2 CFU mL −1 from infections. Another method to induce oral candidosis was used by Totti et al 33 In this study, the major salivary glands of rats were surgically removed to induce this infection.…”

“…22,33 Teichert et al 22 used a protocol of severe combined immunodeficiency disease (SCID) to induce oral candidosis and recovered approximately 2 × 10 2 CFU mL −1 from infections. Another method to induce oral candidosis was used by Totti et al 33 In this study, the major salivary glands of rats were surgically removed to induce this infection. The infection was identified by the presence of lesions on the dorsum of the tongue which were confirmed by the histopathological analysis.…”