Subcutaneous tissue reaction to castor oil bean and calcium hydroxide in rats

Camargo, Samira Esteves Afonso; Rode, Sigmar de Mello; Prado, Renata Falchete do; Carvalho, Yasmin Rodarte; Camargo, Carlos Henrique

doi:10.1590/s1678-77572010000300014

Cited by 12 publications

(15 citation statements)

References 22 publications

(38 reference statements)

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“…Lymphocytes were considered the cells with small, round, and hyperchromatic nuclei, revealing a lack of cytoplasm. By contrast, the macrophages presented ample, hyperchromatic, and oval or bean-shaped nuclei, with a greater quantity of cytoplasm [13].…”

Section: Methodsmentioning

confidence: 82%

Effect of Physical Activity on the Lesion Repair Process in the Gastrocnemius Muscle of Rats

Namba¹,

Nawick²,

Thome

et al. 2014

TOINFJ

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Abstract:The present study aimed to evaluate the effect of a progressive load of physical activity on a musculoskeletal injury in rats. Sixty-four rates were divided into two groups: experimental group (EG), which underwent physical activity (swimming) with a progressive load, and the control group (CG), which was not submitted to this program. The training was carried out according to an adapted version of the Gonçalves (1999) swimming system. Injuries were caused to the gastrocnemic muscle by inducing 40% NaOH. On days 2, 7, 14, and 21 after inducing muscle injury, the animals from both EC and CG were sacrificed. The injured area was removed and processed. The neutrophils, macrophages, lymphocytes, and plasmocytes were quantified by hematoxylin and eosin (H&E) stain. The percentage of the area of type I and type III collagen were quantified by a histological cut stained with picrosirius red stain (PSR) and submitted to polarization. A greater number of macrophages (GE=19.98±4.39; GC=2.48±1.00; p=0.006) and lymphocytes (GE=2.12±0.82; GC=0.06±0.82; p=0.037) after 2 days, and of macrophages (GE=15.74±3.00; GC=6.02±1.95; p=0.007) and lymphocytes (GE=2.01±0.78; GC=0.14±0.09; p=0.044) after 7 days, could be observed in the EG when compared to the CG; however, the opposite was true for neutrophils (GE=48.12±17.04; GC=105.54±12.25; p=0.005). After 14 days, a smaller quantity of neutrophils (GE= 32.70±10.26; GC= 90.96±17.62; p= 0.044) and a larger quantity of plasmocytes (GE=9.06±3.84; GC=0.68±0.53; p=0.028) could be observed in the EG as compared to the CG. A greater area of type III collagen could be observed in the EG when compared to the CG over a 14-day period (GE=44.90±16.15; GC=0.74±0.40; p=0.000) and a 21-day period (GE=13.19±9.09; GC=1.02±0.94; p=0.029), whereas the opposite could be observed for type I collagen. The physical activity promoted an increase in the deposition of type III collagen in the muscle injury. This activity accelerated the repair process after 14 days, possibly moderated by the larger number of inflammatory cells, while after 21 days, the lesion presented a lesser resistance.

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Section: Methodsmentioning

confidence: 82%

Effect of Physical Activity on the Lesion Repair Process in the Gastrocnemius Muscle of Rats

Namba¹,

Nawick²,

Thome

et al. 2014

TOINFJ

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“…Some studies (CHANG et al, 1998;CAMARGO et al, 2009;SEPET et al, 2009;SILVA et al, 2012A;SILVA et al, 2012B;YOSHINO et al, 2013) used fibroblasts for quick and easy growth (HIRSHMAN et al, 2012), in addition to their availability in periapical tissues and the periodontal ligament, which are areas that are susceptible to the effects of intracanal medications and their degradation products (YOSHINO et al, 2013). Fibroblasts are also the largest producers of collagen tissue and, therefore, actively participate in the repair tissue process (SILVA et al, 2012B).…”

Section: Discussionmentioning

confidence: 99%

“…The rationale for the observed cytotoxicity may be the extremely alkaline environment, which promotes enzymatic denaturation and destruction of the cell membrane, leading to cell death (GUIGAND et al, 1999;SEPET et al, 2009;SILVA et al, 2012B). In animals, this cytotoxic action occurs when calcium hydroxide comes in direct contact with subcutaneous tissue, inducing inflammatory reactions of moderate to severe intensity and resulting in protein denaturation in the cells, especially of neutrophils, and areas of necrosis and dystrophic calcification (NELSON FILHO et al, 1999;CAMARGO et al, 2010;TOMAZ et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…incubation period ensured that the active products of the intracanal medications could be diffused into the culture medium, producing eluates of each intracanal medication (CAMARGO et al, 2010). For each group, three eluates were produced, even for the control group.…”

Section: Preparation Of Eluatesmentioning

confidence: 99%

“…Otherwise these products can cause high levels of inflammation, mediated by a number of factors including histamines, kinins, and neuropeptides, which can induce tissue destruction and slow the cicatricial process (YOSHINO et al, 2013). Therefore, these drugs should possess the ability to induce repair in the injured area without interfering with osteogenesis and cementogenesis (CAMARGO et al, 2009;SILVA et al, 2012A).…”

Section: Introductionmentioning

confidence: 99%

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Assessment of intracanal medications cytotoxicity on L929 fibroblast cells

Farias¹,

Matos²,

Carvalho³

et al. 2016

Biosci. J.

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ABSTRACT:This study aimed to evaluate the cytotoxicity of intracanal medications on L929 fibroblast cells at different periods of observation. The following experimental groups were studied: calcium hydroxide with camphorated paramonochlorophenol and glycerin (CPG); iodoform with glycerin (IG); calcium hydroxide with iodoform and distilled water (CIW); iodoform with distilled water (IW); calcium hydroxide with distilled water (CW); Otosporin ® (OT); and a control group composed of cells and culture medium. Eluates were prepared from each group and placed in contact with 1 x 10 5 cells/well for periods of 30 minutes, 12, 24, 48 and 72 hours, 5 and 7 days. After each experimental period, a cytotoxicity test was performed using methyltetrazolium (MTT) and a spectrophotometer at an optical density of 570 nm to analyze cell viability. The ANOVA and Tukey test with a significance level of 5% was used to analyze the data. At 30 minutes and at 12 hours, all groups were equal to the control group. At 24 hours, there was greater cytotoxicity in the IG group than in the control group (P<0.001). At 48 hours, only the OT group was cytotoxic (P <0.001). At 72 hours and at 5 days, the most cytotoxic groups were CW and OT. At 7 days, the IW and CPG groups were the least cytotoxic (P <0.001). With respect to experimental time, significant differences between 24 hours and 7 days were observed in all groups. Otosporin ® was the most cytotoxic medication, followed by calcium hydroxide with distilled water.

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Carbon fiber: Characterization and evaluation of the inflammatory response and toxicity in rats

et al. 2023

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This study aimed to evaluate the Carbon Fiber obtained from PAN textile and cotton fiber in their different forms of presentation: non‐activated carbon fiber felt (NACFF), activated carbon fiber felt (ACFF), silver activated carbon fiber felt (Ag‐ACFF), and activated carbon fiber tissue (ACFT), to obtain scaffolds as a potential material with properties related to the synthetic bone graft. Characterization tests performed: surface wettability, traction, swelling, and in vivo tests: evaluation of the inflammatory response by implanting the materials in the subcutaneous tissue of 14 Wistar rats, evaluation of collagen fibers by picrosirius red staining and assessment of toxicity in the following organs: heart, spleen, liver, and kidney. In the wettability test, NACFF and ACFT were hydrophobic (θ124° and 114°), ACFF and Ag‐ACFF were hydrophilic. For maximum stress, ACFF was more resistant (2.983 ± 1.059) p < .05. In the swelling test, the Ag‐ACFF and ACFF groups showed the highest absorption percentage for the PBS solution and distilled water (p < .001). The organs showed no signs of acute systemic toxicity. The implant regions showed mild to moderate inflammatory infiltrate at 7 and 21 days. Only the ACFT group did not show the maturation of type I collagen fibers in 21 days. Through the conducted analyses, the ACFT shows little potential to be indicated as a possible scaffold. Therefore NACFF, ACFF, and Ag‐ACFF have the potential to be considered scaffolds due to the following characteristics presented: good absorption rate, hydrophilicity, and non‐toxic.

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Subcutaneous tissue reaction to castor oil bean and calcium hydroxide in rats

Cited by 12 publications

References 22 publications

Effect of Physical Activity on the Lesion Repair Process in the Gastrocnemius Muscle of Rats

Effect of Physical Activity on the Lesion Repair Process in the Gastrocnemius Muscle of Rats

Assessment of intracanal medications cytotoxicity on L929 fibroblast cells

Carbon fiber: Characterization and evaluation of the inflammatory response and toxicity in rats

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