Effects of different drying rates on the physiological quality of Coffea canephora Pierre seeds

Rosa, Sttela Dellyzete Veiga Franco da; Júnior, Delacyr da Silva Brandão; Pinho, Édila Vilela de Resende Von; Veiga, André Delly; Silva, Luiz Hildebrando de Castro e

doi:10.1590/s1677-04202005000200002

Cited by 28 publications

(28 citation statements)

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“…The benefits of slow drying were also reported for other species with seeds of intermediate behavior, such as Magnolia ovata (José et al, 2011) and Coffea canephora (Rosa et al, 2005), which exhibited lower sensitivity to desiccation at lower water contents.…”

Section: Resultsmentioning

confidence: 54%

Physiological behavior of Genipa americana L. seeds regarding the capacity for desiccation and storage tolerance

et al. 2013

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-Knowledge of the capacity of desiccation and storage tolerance of seeds is essential to provide adequate conditions necessary to maintain viability after seed collection. Drying rate is one of the factors which have been described as affecting the physiological response of seeds of various species. Literature reports that Genipa americana seeds exhibit an intermediate behavior, quickly losing viability if stored at negative temperatures. Thus, this work aimed to verify the effect of slow drying over the capacity of desiccation and storage tolerance of Genipa americana seeds. Seeds with 47% of water content (recently harvested) were dried over salt solutions until reaching 30%, 20%, 15%, 10% and 5% of water content. After drying to 10% and 5% of water content a reduction in seed viability was observed after 30 days of storage without the total loss of germination after three months in these conditions. The slow drying increased the capacity for desiccation and storage tolerance of the Genipa americana seeds if compared to the information described in literature.Index terms: physiological classification, brazilian forest species, genipapo plants.Comportamento fisiológico de sementes de Genipa americana L.quanto à tolerância à dessecação e ao armazenamento RESUMO -Conhecer a capacidade de tolerância à dessecação e ao armazenamento das sementes tem sido imprescindível para disponibilizar condições adequadas para a manutenção da viabilidade após a coleta. A velocidade de secagem é um dos fatores que tem influenciado na resposta fisiológica das sementes de diversas espécies. Deste modo, buscou-se verificar o efeito da secagem lenta na capacidade de tolerância à dessecação e ao armazenamento das sementes de Genipa americana L. Sementes com 47% de umidade (recém-colhidas) foram secas em soluções salinas até 30, 20, 15, 10 e 5% de umidade, testando-se a viabilidade. Ao atingir 10 e 5% de umidade as sementes foram armazenadas a -20 ºC, avaliando-se a viabilidade a cada 30 dias durante três meses. Sementes recém-colhidas apresentaram 98% de germinação sem perda de viabilidade até 10% de umidade. Sementes secas a 10 e 5% de umidade apresentaram redução na viabilidade após 30 dias de armazenamento, porém não houve a perda total de germinação após este período. A secagem lenta aumentou a capacidade de tolerância à dessecação e ao armazenamento das sementes de Genipa americana se comparada às informações descritas na literatura.Termos para indexação: classificação fisiológica, espécie florestal brasileira, jenipapeiro.

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Section: Resultsmentioning

confidence: 54%

Physiological behavior of Genipa americana L. seeds regarding the capacity for desiccation and storage tolerance

et al. 2013

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“…The authors did not observe any significant difference in germination and vigor of fresh seeds (50% wb) with those subjected to quick drying (15% wb). Rosa et al (2005) also observed that moisture level affects the physiological quality of C. canephora Pierre seeds in the radicle protrusion variable soon after drying; however, interaction with the storage effect is necessary to cause significant differences in the germination variable at 30 days. These differences might be explained by the different genetic and methodological techniques used in the current study compared to those of Rosa et al (2005).…”

Section: Discussionmentioning

confidence: 90%

“…In addition, while CAT expression also reduced in seeds with intermediate moisture levels, it increased in seeds with 12% wb moisture level (Figure 2). This difference might be associated with the different drying rates in the 2 methods (Rosa et al, 2005). CAT gene expression did not appear to have a clear relationship with physiological quality.…”

Section: Gene Expression Of the Oxidative Process By Rt-qpcrmentioning

confidence: 83%

“…However, existing studies about seed behavior after drying and storage have produced contradictory and inconclusive results, and primarily focus on identifying the best method of drying in relation to the final moisture level of the seeds (Rosa et al, 2011). The drying speed of the seeds also influences their physiological quality, with different studies recommending both slow drying (Brandão Júnior et al, 2002;Vieira et al, 2007) and quick drying (Rosa et al, 2005;Dussert et al, 2006) as the best drying method. Differences in the levels of tolerance to desiccation also exist among different species of Coffea (Eira et al, 1999), with the species Coffea racemosa being perceived as the most tolerant, and C. liberica as the most sensitive, while C. arabica and C. canephora are intermediate.…”

Section: Introductionmentioning

confidence: 99%

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Gene expression of coffee seed oxidation and germination processes during drying

Santos¹,

Pinho²,

Rosa³

2013

Genet. Mol. Res.

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ABSTRACT. Coffee (Coffea arabica L.) seeds are sensitive to desiccation and oxidative stress during drying processes. We investigated the effect of drying and moisture levels on germinationrelated gene expressions associated with enzymatic systems that prevent oxidative stress in coffee seeds. Coffee seeds collected at physiological maturity were subjected to slow and quick drying to 40, 30, 20, and 12% moisture levels (wet basis), and as the control, seeds without drying were used. The seeds' physiological quality was calculated as percentage of normal seedlings at 15 and 30 days, normal vigorous seedlings at 30 days, and cotyledonary leaves at 45 days. The isoenzymes esterase, catalase (CAT), peroxidase (POX), and endo-β-mannanase expressions were electrophoretically analyzed. CAT and POX expressions were analyzed using RT-qPCR with specific primers constructed from the target gene sequences from the Brazilian Coffee Genome Database. Slow drying showed better physiological quality for seeds at 40 and 12% moisture levels, while quick drying was the most effective for seeds with 20% moisture. Sensitivity to water loss was confirmed by quick drying and activation of enzymes. CAT and POX transcriptions reduced during drying. RT-qPCR revealed a 6969 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 12 (4): 6968-6982 (2013) Gene expression during coffee seed drying process complex gene-expression pattern during the oxidative process, with high gene expression in wet seeds.

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“…According to Magistrali et al (2013), slow drying increased desiccation capacity and storage tolerance in studies with Genipa americana seeds. Furthermore, in coffee seeds (Coffea anefora), fast drying caused a greater reduction in physiologic quality compared to slow and intermediate drying (ROSA et al, 2005). Ocotea puberula specie have their seeds classified as recalcitrant as pointed out by Mori et al (2012).…”

Section: Introductionmentioning

confidence: 99%

SLOW AND FAST DRYING IN SEEDS OF Ocotea puberula (Rich.) Ness

et al. 2016

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-The objective of this work was to evaluate the influence of types of drying on seeds' quality, as recalcitrant, Ocotea puberula and determine their degree of critical humidity and lethal degree. Seeds harvested in Brunópolis, SC, with an initial moisture content of 38%, were dried to 18%, with gradients of 2%; using the equation of target weight to ensure that gradient in the stove (35 oC) and in a desiccator with silica gel (25 oC). After drying, the seeds were evaluated for water content, percentage and germination speed index (GSI), tetrazolium and T50. It was observed that up to 32% water content did not change in seed quality, regardless of the type of drying and verified significant loss of germination after this value. We conclude that the type of drying does not affect the quality of the seed; however, because it is a recalcitrant seed, reducing the water content below 32% decreased germination, and its degree of critical humidity and seeds with 22% moisture content had no germination in this study is the degree of lethal humidity of this species.Key-words: Canela-guaicá; Recalcitrant seed; Water content.SECAGEM LENTA E RÁPIDA EM SEMENTES DE Ocotea puberula (Rich.) Ness RESUMO -Objetivou-se avaliar a influência de tipos de secagem na qualidade de sementes, consideradas recalcitrantes, de Ocotea puberula e determinar o seu grau de umidade crítico e grau letal. Sementes colhidas em Brunópolis, SC, com teor de água inicial de 38%, foram secas até 18%, com gradientes de 2%; utilizando a equação de peso alvo para garantir este gradiente, em estufa (35 oC) e em dessecador com sílica-gel (25 oC). Após cada secagem, as sementes foram avaliadas quanto ao teor de água, porcentagem e velocidade (IVG) de germinação, T50 e tetrazólio. Foi observado que até 32% de teor de água não houve alteração na qualidade das sementes, independente do tipo de secagem, sendo verificada significativa perda de germinação após esse valor. Conclui-se que o tipo de secagem não influencia na qualidade dessas sementes; porém, por tratar-se de uma semente recalcitrante, a redução do teor de água abaixo de 32% prejudica a germinação, sendo seu grau de umidade crítico e sementes com 22% de teor de água não tiveram germinação, sendo neste estudo o grau de umidade letal desta espécie.Palavras-chave: Canela-guaicá; Semente recalcitrante; Teor de água.

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Effects of different drying rates on the physiological quality of Coffea canephora Pierre seeds

Cited by 28 publications

References 20 publications

Physiological behavior of Genipa americana L. seeds regarding the capacity for desiccation and storage tolerance

Physiological behavior of Genipa americana L. seeds regarding the capacity for desiccation and storage tolerance

Gene expression of coffee seed oxidation and germination processes during drying

SLOW AND FAST DRYING IN SEEDS OF Ocotea puberula (Rich.) Ness

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