Physiological behavior of Genipa americana L. seeds regarding the capacity for desiccation and storage tolerance

Magistrali, Paulo Roberto; José, Anderson Cleiton; Faria, José Márcio Rocha; Gasparin, Ezequiel

doi:10.1590/s2317-15372013000400011

Cited by 19 publications

(29 citation statements)

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“…These enzymes are essential, since they provide preparatory substrates for a rapid expansion of the embryonic axis, immediately after the perception of light stimuli (Goggin et al, 2011). Different studies on the G. americana germination have shown that the germination process is highly desuniform and can vary between 45 and 90 days (Magistrali et al, 2013;Magistrali et al, 2015). In contrast, the present study showed that the stratification pretreatment in the dark for a period of 16 days resulted in 100% germination, with an average time of 30 days, indicating that the use of this pretreatment is required, followed by a transfer to the 16 h photoperiod with light provided by white light, so that G. americana seeds express their maximum germination potential.…”

Section: Stratification Pretreatment In the Darkmentioning

confidence: 99%

“…However, there are several limitations that prevent its commercial exploitation as: lack of varieties and selected seedlings; seeds sensitivity to dehydration, with loss of viability in the short term; slow, low and uneven germination (Magistrali et al, 2013;Santos;Silva-Mann;Ferreira, 2011). Thus, in vitro seed germination is the most effective way for quickly obtaining explants with sanitary quality.…”

Section: Introductionmentioning

confidence: 99%

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Optimization of jenipapo in vitro seed germination process

et al. 2016

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The in vitro seed germination is an effective alternative for quickly obtaining explants with sanitary quality. However, jenipapo seeds present slow and uneven germination. Therefore, internal and external factors to seed which directly interfere in the process, they must be identified, in order to adapt better techniques to obtain seedlings. In this sense, this work aimed to optimize the in vitro germination of Genipa americana L. seeds by evaluating different factors (light quality, GA 3 treatment, pre-soaking in distilled water, growing media and stratification in the dark). It was found that the seed germination of G. americana was indifferent to light, however, the best results were obtained under conditions of continuous darkness; There was no effect of the application of exogenous GA 3 ; The pre-soaking in distilled water for 48 h contributes to obtaining better germination rates; And the reduction in MS medium salts, and laminating the pretreatment in the dark maximizes the germination potential of seeds.Therefore, the optimal conditions for in vitro germination of G. americana L. seeds requires pre-soaking in distilled water for 48 hours and inoculation into culture media consisting of ½ MS + 15 g L -1 sucrose, with stratification in the dark for 16 days, followed by the transfer to growth chambers with lighting provided by white fluorescent lamps.Index terms: Genipa americana L.; in vitro culture; ligth quality; Rubiaceae; stratification in the dark. RESUMOA germinação in vitro de sementes é uma alternativa eficaz para obtenção de explantes com rapidez e qualidade sanitária. No entanto, sementes de jenipapeiro apresentam germinação lenta e desuniforme. Para tanto, fatores internos e externos às sementes que interferem diretamente no processo, devem ser identificados, visando à adaptação de melhores técnicas para obtenção de mudas. Neste sentido, objetivou-se otimizar a germinação in vitro de sementes de Genipa americana L. por meio da avaliação de diferentes fatores (qualidade de luz, tratamento com GA 3 , pré-embebição em água destilada, diferentes composições no meio de cultivo e estratificação no escuro). Verificou-se que a germinação das sementes foi indiferente a luz, no entanto, os melhores resultadados foram obtidos sob condições de escuro contínuo; Não houve efeito da aplicação de GA 3 exógeno; A pré-embebição em água destilada durante 48h contribui para obtenção de melhores taxas de germinação; E a redução de sais do meio MS, bem como o pré-tratamento de estratificação no escuro maximizam o potencial germinativo das sementes. Portanto, as condições ótimas para germinação in vitro de sementes de G. americana L. requer a pré-embebição em água destilada por 48 horas e a inoculação em meio de cultivo constituído de ½ MS + 15 g L-1 de sacarose, com estratificação no escuro por 16 dias, seguida pela transferência a salas de crescimento com iluminação fornecida por lâmpadas fluorescentes branca.Termos para indexação: Genipa americana L.; cultivo in vitro; qualidade de luz; Rubiaceae; estratif...

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Section: Stratification Pretreatment In the Darkmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Optimization of jenipapo in vitro seed germination process

et al. 2016

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“…Jenipapo has a great potential to be explored; however, some bottlenecks, such as the lack of varieties and seeds sensitivity to dehydration, with loss of viability in the short term, together with slow and uneven germination still prevent its commercial exploitation (Magistrali et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Substrates for Jenipapo (Genipa americana L.) Seedlings Production

Silva¹,

Soares²,

Cardoso³

et al. 2018

JAS

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Jenipapo (Genipa americana L. Rubiaceae) is a native species not endemic to Brazil and found in several biomes. The objective of this work was to evaluate the emergence and growth of seedlings cultivated in different substrates. Treatments were made of: T1 (fertilized soil), T2 (fertilized soil + coconut fiber), T3 (fertilized soil + sand), T4 (sand + coconut fiber), T5 (bovine manure + coconut fiber), and T6 (bovine manure + sand). The experiment consisted of a completely randomized design, with six treatments (substrates) and four replications of 25 seeds. The following variables were evaluated: first emergence count, percentage of emergence, emergence speed index, seedling height, root length, stem diameter, number of leaves, relative chlorophyll content (SPAD), leaf area, root dry matter, and shoot dry matter. The substrates T1 (fertilized soil), T2 (fertilized soil + coconut fiber), T3 (fertilized soil + sand) and T6 (bovine manure + sand) were more efficient for jenipapo seedlings production.

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“…According to Magistrali et al (2013), slow drying increased desiccation capacity and storage tolerance in studies with Genipa americana seeds. Furthermore, in coffee seeds (Coffea anefora), fast drying caused a greater reduction in physiologic quality compared to slow and intermediate drying (ROSA et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

SLOW AND FAST DRYING IN SEEDS OF Ocotea puberula (Rich.) Ness

et al. 2016

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-The objective of this work was to evaluate the influence of types of drying on seeds' quality, as recalcitrant, Ocotea puberula and determine their degree of critical humidity and lethal degree. Seeds harvested in Brunópolis, SC, with an initial moisture content of 38%, were dried to 18%, with gradients of 2%; using the equation of target weight to ensure that gradient in the stove (35 oC) and in a desiccator with silica gel (25 oC). After drying, the seeds were evaluated for water content, percentage and germination speed index (GSI), tetrazolium and T50. It was observed that up to 32% water content did not change in seed quality, regardless of the type of drying and verified significant loss of germination after this value. We conclude that the type of drying does not affect the quality of the seed; however, because it is a recalcitrant seed, reducing the water content below 32% decreased germination, and its degree of critical humidity and seeds with 22% moisture content had no germination in this study is the degree of lethal humidity of this species.Key-words: Canela-guaicá; Recalcitrant seed; Water content.SECAGEM LENTA E RÁPIDA EM SEMENTES DE Ocotea puberula (Rich.) Ness RESUMO -Objetivou-se avaliar a influência de tipos de secagem na qualidade de sementes, consideradas recalcitrantes, de Ocotea puberula e determinar o seu grau de umidade crítico e grau letal. Sementes colhidas em Brunópolis, SC, com teor de água inicial de 38%, foram secas até 18%, com gradientes de 2%; utilizando a equação de peso alvo para garantir este gradiente, em estufa (35 oC) e em dessecador com sílica-gel (25 oC). Após cada secagem, as sementes foram avaliadas quanto ao teor de água, porcentagem e velocidade (IVG) de germinação, T50 e tetrazólio. Foi observado que até 32% de teor de água não houve alteração na qualidade das sementes, independente do tipo de secagem, sendo verificada significativa perda de germinação após esse valor. Conclui-se que o tipo de secagem não influencia na qualidade dessas sementes; porém, por tratar-se de uma semente recalcitrante, a redução do teor de água abaixo de 32% prejudica a germinação, sendo seu grau de umidade crítico e sementes com 22% de teor de água não tiveram germinação, sendo neste estudo o grau de umidade letal desta espécie.Palavras-chave: Canela-guaicá; Semente recalcitrante; Teor de água.

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Physiological behavior of Genipa americana L. seeds regarding the capacity for desiccation and storage tolerance

Cited by 19 publications

References 18 publications

Optimization of jenipapo in vitro seed germination process

Optimization of jenipapo in vitro seed germination process

Evaluation of Substrates for Jenipapo (Genipa americana L.) Seedlings Production

SLOW AND FAST DRYING IN SEEDS OF Ocotea puberula (Rich.) Ness

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