Effects of some parameters in upscale culture of Haematococcus pluvialis Flotow

Abstract

Growth rate and medium parameters between two bench scale volumes (13-L and 250-L) were compared. Experiments were maintained batch mode and culture parameters were periodically measured during a 13-day period. Culture growth during the cultivation of algae Haematococcus pluvialis was determined qualitatively by cell counting, optical density, dry weight, ash content, amount of chlorophyll-a, total organic carbon content and by direct measuring of medium nutrients and some abiotic aspects. Vegetative cell growth was higher when cultured in 13-L with 1.33 x 10 5 cells.mL -1 on the 12 th day than when cultured in 250-L. Significant difference (p < 0.05) in the biology and water culture of H. pluvialis, with the exception of dry weight, ash, nitrite and ammonia, was reported between the volumes. Data obtained in current study for the upscale culture maintenance of H. pluvialis in laboratory conditions shows that it should be undertaken in a 13-L volume due to a greater time span of cells in a vegetative state, greater cell density, lipids and chlorophyll-a contents. Light was of paramount importance on the direct performance of H. pluvialis on the algal biological conditions. Keywords: biological aspects, growth, nutritional content, microalgae. Efeito de alguns parâmetros no cultivo em larga escala de Haematococcus pluvialis Flotow ResumoForam avaliadas a taxa de crescimento e condições do meio de cultura em volumes de 13-L e 250-L em sistema estático, durante o período de 13 dias para a microalga Haematococcus pluvialis. Foi determinada qualitativamente a contagem de células, densidade, peso seco, teor de cinzas, clorofila-a, teor de carbono orgânico total e avaliação de nutrientes e fatores abióticos do meio de cultura. O crescimento foi mais elevado em volume de 13-L com 1,33 x 10 5 células.mL -1 no décimo segundo dia, do que em volume de 250-L. Em relação ao meio de cultivo e aspectos biológicos de H. pluvialis, foram observadas diferenças significativas (p < 0,05) entre os dois volumes com exceção do peso seco, cinzas, nitrito e amônia. Para cultivo em larga escale de H. pluvialis é recomendado nestas condições laboratoriais, o volume de 13-L devido ao maior tempo das células em estado vegetativo, maior densidade celular e elevados teores de lipídios e clorofila-a. A luz interferiu diretamente nas condições biológicas de H. pluvialis. Palavras-chave: aspectos biológicos, crescimento, conteúdo nutricional, microalga.

Propagation of Inoculum for Haematococcus pluvialis Microalgae Scale-Up Photobioreactor Cultivation System

“…The doubling time of microalgae biomass was reached on the fifth and sixth day of the cultivation, which is comparable to the results of research [20], in which in the 12 L photobioreactor, the doubling of the number of Haematococcus pluvialis microalgae cells was reached on day 6.9.…”

“…However, the system used for monitoring temperature allowed for controlling it, which is very important for maintaining the highest possible productivity [19]. The results of measurements collected over the course of experiments confirmed that the work of the station in an air-conditioned room at a constant temperature of 22 • C prevents the upper-temperature threshold from being exceeded, as proposed by other authors, e.g., in [20,21]. The systematic increase of temperature over the course of the experiments (Figures 6 and 9) was caused by the heat released by the LED lighting installed inside each storage unit.…”

“…The utilized way of scaling the culture by a factor of 10 per step proved itself in practice. Admittedly, many researchers use higher factors (20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30) when scaling-up cultures [35][36][37]. However, in that way they are breaking the practical rule used, i.e., by fermentation technology, which says that each increase of scale should not surpass a factor of 10.…”

Stimulated Growth of Green Algae Haematococcus Pluvialis Under Different Environmental Conditions

“…Sipaúba-Tavares et al (2015), in the 2-L experiments in NPK and M+NPK alternative media with high nitrogen and phosphorus contents, associated higher cell concentration in NPK medium (light intensity 20 and 60 µmol.m 2 .s -1 ) to phosphate (orthophosphate and total phosphorus) in the medium. Improved algae growth in upscaled cultures (13-L) from the 9 th day assumably was due to total phosphorus and orthophosphate increase (Sipaúba-Tavares et al, 2013).…”