Potential of Metarhizium anisopliae (Metsch.) Sorokin (Ascomycetes, hypocreales) in the control of Bonagota salubricola (Meyrick) (Lepidoptera, Tortricidae) and its compatibility with chemical insecticides

Anhalt, Fransoê Alan; Azevedo, João Lúcio de; Sugayama, Regina L.; Specht, Alexandre; Barros, Neiva Monteiro de

doi:10.1590/s1519-69842010000500003

Cited by 7 publications

(3 citation statements)

References 14 publications

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“…This incompatibility has been reported by studies which have demonstrated the influence of chemicals on entomopathogenic fungus viability (Barci et al, 2009) although, in another study, the fungus and insecticide employees were considered compatible, allowing their joint use in controlling (Anhalt et al, 2010). Thereby, further studies to evaluate the alterations in nematophagous fungus development caused by the combined use with antiparasitic drugs are needed.…”

Section: Discussionmentioning

confidence: 87%

In vitro susceptibility of nematophagous fungi to antiparasitic drugs: interactions and implications for biological control

et al. 2016

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The fast anthelmintic resistance development has shown a limited efficiency in the control of animal's endoparasitosis and has promoted research using alternative control methods. The use of chemicals in animal anthelmintic treatment, in association with nematophagous fungi used for biological control, is a strategy that has proven to be effective in reducing the nematode population density in farm animals. This study aims to verify the in vitro susceptibility of the nematophagous fungi Arthrobotrys oligospora, Duddingtonia flagrans and Paecilomyces lilacinus against the antiparasitic drugs albendazole, thiabendazole, ivermectin, levamisole and closantel by using the Minimum Inhibitory Concentration (MIC). MICs ranged between 4.0 and 0.031 µg/mL for albendazole, thiabendazole and ivermectin, between 0.937 and 0.117 µg/mL for levamisole, and between 0.625 and 0.034 µg/mL for closantel. The results showed that all antiparasitic drugs had an in vitro inhibitory effect on nematophagous fungi, which could compromise their action as agents of biological control. D. flagrans was the most susceptible species to all drugs.Keywords: nematophagous fungi, benzimidazoles, ivermectin, levamisole, closantel. Suscetibilidade in vitro de fungos nematófagos à antiparasitários: interações e implicações para o controle biológicoResumo O desenvolvimento rápido da resistência anti-helmíntica demonstrou a eficiência limitada no controle de endoparasitoses em animais, e promoveu a investigação em métodos de controles alternativos. O uso de produtos químicos no tratamento anti-helmíntico animal, em associação com fungos nematófagos utilizados para o controlo biológico, é uma estratégia que tem provado ser eficaz na redução da densidade da população de nematódeos em animais agrícolas. Este estudo teve como objetivo verificar a suscetibilidade in vitro dos fungos nematófagos Arthrobotrys oligospora, Duddingtonia flagrans e Paecilomyces lilacinus frente aos antiparasitários albendazol, tiabendazol, ivermectina, levamisol e closantel, usando a concentração inibitória mínima (MIC). Os MICs variaram entre 4,0 e 0,031 μg/mL para albendazol, tiabendazol e ivermectina, entre 0,937 e 0,117 μg/mL para o levamisol, e entre 0,625 e 0,034 μg/mL para closantel. Os resultados mostraram que todos os antiparasitários tiveram um efeito inibidor in vitro sobre os fungos nematófagos, o que poderia comprometer suas atividades como agentes de controle biológico. D. flagrans foi a espécie mais sensível a todas as drogas.Palavras-chave: fungos nematófagos, benzimidazóis, ivermectina, levamisol, closantel.

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Section: Discussionmentioning

confidence: 87%

In vitro susceptibility of nematophagous fungi to antiparasitic drugs: interactions and implications for biological control

et al. 2016

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“…That is because of the difference in genders, although it may even occur in the same lineage of fungi. The natural genetic variability between isolates of the same species of fungus is well known and widely reported in the literature for several biological parameters, including for sensitivity to synthetic chemical products (Liu et al, 1993;Anhalt et al, 2010).…”

Section: Resultsmentioning

confidence: 99%

Sensitivity of hyperparasitic fungi to alternative products for use in the control of papaya black spot

et al. 2021

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The use of more than one control technique can maximize the reduction of the damages caused by the fungus Asperisporium caricae causal agent of the black spot in the papaya crop. The objective of this work was to evaluate the sensitivity of the fungi Hansfordia pulvinata and Acremonium spp. to alternative products with potential for use in the control of the black-spotted ptarmigan. Three isolates of Acremonium spp. (A-598, A-602 and A-617) and an isolate of H. pulvinata (H-611) were grown in BDA medium containing Agro-Mos  , Bion  , Ecolife  , Hortifospk  , Matriz G  , Vitaphol  separately. The Amistar 500WG  fungicide was used as a positive control and pure BDA as a negative control. The toxicity of the tested products was determined based on the values of the biological index, derived from the means of mycelial growth, sporulation and germination of conidia, in each experimental unit. In this way it was possible to select the products classified as compatible for all isolates, and to test them in vivo. In the greenhouse, only the isolates and isolates with selected products, were applied in papaya plants with foliar symptoms of black-spotted. The incidence of leaves with hyperparasites and the percentage of black-painted lesions colonized by the tested isolates were evaluated. Thus, the H-611 isolate proved to be compatible with most of the alternative products tested, except with Hortifos  . Bion  and Matrix  products were compatible with all tested isolates and could be used in conjunction with Acremonium spp. and H. pulvinata to control the papaya black spot, since these products did not present toxicity on the hyperparasitic fungi.

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“…Experiments have been conducted in the detection of a pesticide effect on entomopathogenic fungi (Anhalt et al, 2010;Oliveira and Neves, 2004;Alizadeh et al, 2007). Borges and Vila Nova (2011) reported the occurrence of physiological alterations which includes the loss of entomopathogenic fungus performance activity on arthropods after the use of chemicals.…”

Section: Discussionmentioning

confidence: 99%

Antiparasitic drugs: in vitro tests against nematophagous fungi

et al. 2016

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The use of biological agents has been intensified in recent years against eggs and larvae of gastrointestinal nematodes as an alternative control method in pasture plant health management, with the concomitant use with antiparasitic drugs still occurring. The aim of this study was to test the in vitro activity of the following antiparasitic drugs: Ivermectin and albendazole against the following nematophagous fungi: Paecilomyces fumosoroseus, Paecilomyces lilacinus and Paecilomyces variotii. The agar diffusion test was performed using an initial concentration of 0.0016g/mL of each drug, after solidification of the culture medium containing the drug concentration each nematophagous fungi was inoculated. The results showed that in a concentration of 80μg/mL, the fungal growth decreased, however, with the concentration of 160μg/mL, there was no fungal growth in both drugs, compared to the control, which indicates an inhibition in the development of the nematophagous fungi studied when they come in contact with ivermectin and albendazole.Keywords: ivermectin, albendazole, biological control. Antiparasitários: teste in vitro contra fungos nematófagosResumo O uso de agentes biológicos que atuam em ovos e larvas de nematódeos gastrintestinais como uma alternativa para o manejo de pastagens de saúde tem se intensificado nos últimos anos, bem como o uso concomitante com outros medicamentos antiparasitários. O objetivo deste estudo foi testar o efeito in vitro dos fármacos Ivermectina e Albendazol em fungos nematófagos Paecilomyces fumosoroseus, Paecilomyces lilacinus e Paecilomyces variotii. Foi utilizada a técnica de difusão em agar, sendo preparado a partir de uma concentração inicial de 0,0016g/mL de cada uma das drogas e diluídas em meio de cultura, com posterior semeadura dos fungos nematófagos. Os resultados mostraram que na concentração de 80μg/mL, o crescimento diminuiu, no entanto, com a concentração de 160μg/mL de ambas as drogas, não houve crescimento de fungos durante o período de estudo, em comparação com o controle, indicando a inibição do desenvolvimento dos fungos nematófagos estudados quando em contato com a Ivermectina e Albendazol.Palavras-chave: ivermectina, albendazol, controle biológico.

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Potential of Metarhizium anisopliae (Metsch.) Sorokin (Ascomycetes, hypocreales) in the control of Bonagota salubricola (Meyrick) (Lepidoptera, Tortricidae) and its compatibility with chemical insecticides

Cited by 7 publications

References 14 publications

In vitro susceptibility of nematophagous fungi to antiparasitic drugs: interactions and implications for biological control

In vitro susceptibility of nematophagous fungi to antiparasitic drugs: interactions and implications for biological control

Sensitivity of hyperparasitic fungi to alternative products for use in the control of papaya black spot

Antiparasitic drugs: in vitro tests against nematophagous fungi

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