Molecular key to seven Brazilian species of Trichogramma (Hymenoptera: Trichogrammatidae) using sequences of the ITS2 region and restriction analysis

Abstract

RESUMO -Utilizando-se o sequenciamento da região ITS2 do DNA ribossomal juntamente com algumas enzimas de restrição, pôde-se construir uma chave molecular simples e precisa de algumas espécies brasileiras de Trichogramma. Esta chave é fácil de ser elaborada e resultados rápidos são obtidos na identificação desse pequeno parasitóide (0,25 mm). Usando-se essa metodologia, pode-se também verificar possíveis contaminações em criações de laboratório. PALAVRAS-CHAVE: Insecta, DNA, enzimas, controle biológico.ABSTRACT -Using the ITS2 sequences of the ribosomal DNA together with some restriction enzymes, a simple and precise molecular key to some Brazilian species of Trichogramma was created. This key is very easy to make and quick results can be obtained on the identification of this minute parasitoid (0.25 mm). The methodology presented is easily implemented and can be used to detect possible contaminations under laboratory rearings.

Molecular identification of Trichogramma species from regions in Brazil using the sequencing of the ITS2 region of ribosomal DNA

“…Similar results were obtained by Almeida (2004) andCiociola Junior et al (2001) to identify the species T. galloi, T. exiguum, T. pretiosum using the ITS 2 rDNA region.…”

Parasitoid Biodiversity and Insect Pest Management

“…Genetic markers such as 28S-D2, ITS, and COI are most appropriate for the recognition of insect species or populations. Trichogramma species are minute and difficult to identify based on features of the genitalia, but identification keys to species can be built, using differences in ITS length and sequence divergence (Ciociola et al 2001, Pinto et al 2002. These markers not only are useful for the identification of known species or populations by nonspecialists, but also can be used in studies of larval parasitism.…”

Iranian Trichogramma: ITS2 DNA characterization and natural Wolbachia infection

“…ITS2 is a highly conserved region within species, but varies substantially between species (Silva et al 1999;Stouthamer et al 1999b). Consequently, several molecular keys for the biological important Trichogramma species have been developed based on the length, nucleotide sequence and restriction fragment length polymorphism (PCR-RFLP) analysis of the ITS2 sequences (Chang et al 2001;Ciociola et al 2001;Dang et al 2005;Jeong et al 2010;Kumar et al 2009;Pinto et al 2002;Sappal et al 1995;Silva et al 1999;Stouthamer et al 1999b;Sumer et al 2009; Thomson et al 2003). In some cases, the ITS2 sequence cannot differentiate between species , which makes compatibility experiments based on the biological species concept (Mayr 1942) necessary (Gounou et al 2008;Pinto et al 1991Pinto et al , 1992Pinto andStouthamer 1994, Rosen 1986).…”