Occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk in the northwestern region of Paraná, Brazil

Sgarioni, Sônia Aparecida; Hirata, Rosário Dominguez Crespo; Hirata, Mário Hiroyuki; Leite, Clarice Queico Fujimura; Prince, Karina Andrade de; Leite, Sérgio Roberto de Andrade; Filho, Dirceu Vedovello; Siqueira, Vera Lúcia Dias; Caleffi-Ferracioli, Katiany Rizzieri; Cardoso, Rosilene Fressatti

doi:10.1590/s1517-83822014000200046

Cited by 23 publications

(16 citation statements)

References 27 publications

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“…Literature showed that milk, faeces, and nasal discharges etc. are the routes of excretion of the M. bovis pathogen (Sgarioni et al, 2014). In present study, 2.5% milk samples showed growth on Lowenstein Jensen medium.…”

Section: Prevalence Of Bovine Tb In Relation To Various Factorssupporting

confidence: 55%

Prevalence of bovine tuberculosis in buffaloes in Hyderabad and Tando Allahyar districts of Sindh, Pakistan

Malhi

Kamboh

Kumar

et al. 2018

IJAR

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An investigation in buffaloes was carried out in Hyderabad and Tando Allahyar districts, Sindh province, Pakistan to determine the prevalence of bovine tuberculosis. The buffaloes (n=120) were first screened through single intradermal tuberculin test (SITT), then their sera were used for enzyme-linked immunosorbent assay (ELISA). Attempts were further made to isolate the Mycobacterium bovis organism from the milk samples using traditional culture test. Overall prevalence of 4.16%, 8.33% and 2.5% was recorded by SITT, ELISA and culture test respectively. A somewhat higher prevalence was recorded in Tando Allahyar district (SITT 6.66%; ELISA 10%; culture test 5%) as compared to Hyderabad district (SITT 1.66%; ELISA 6.66%; culture test 0%). Statistical analysis did not show any association (P> 0.05) of herd size, sex, age, milk yield and farming type with the prevalence of the disease. Whereas, SITT showed a significant (P Less than 0.05) association of sex, age and milk yield with the prevalence of the disease in Tando Allayer district. The results of present study revealed that bovine tuberculosis is present in apparently healthy buffalo herds of Hyderabad and Tando Allayer districts. Moreover, infected animals shed the M. bovis pathogen in milk that could be a potential hazard to public health.

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Section: Prevalence Of Bovine Tb In Relation To Various Factorssupporting

confidence: 55%

Prevalence of bovine tuberculosis in buffaloes in Hyderabad and Tando Allahyar districts of Sindh, Pakistan

Malhi

Kamboh

Kumar

et al. 2018

IJAR

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“…Literature shows that there are many roots of excretion of the M. bovis pathogen such as milk, blood, nasal secre-tions, lymph glands, rectal pinch and feces (Srivastava et al, 2006;Sgarioni et al, 2014;Jalil et al, 2003). Therefore, in present study, milk and nasal secretion were collected to isolate M. bovis.…”

Section: Discussionmentioning

confidence: 99%

Isolation of Mycobacterium bovis from Milk and Nasal Discharge Samples of Cattle from Hyderabad and Tando Allahyar Districts

Leghari¹,

Kamboh²,

Dewani³

et al. 2016

J. Anim. Health Prod.

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“…In this study, some of the species we identified have already been detected in cattle in other studies. In Brazil, nine different species of environmental mycobacteria were isolated ( M. nonchro-mogenicum, M. peregrinum, M. smegmatis, M. neoaurum, M. fortuitum, M. chelonae, M. flavescens, M. kansasii and M. scrofulaceum ) in milk from cattle [ 15 ]. Most of the identified Mycobacterium species in this study were also of public health importance as they can potentially cause diseases in humans especially those with AIDS and are immunocompromised.…”

Section: Discussionmentioning

confidence: 99%

Molecular Identification of Mycobacterium Species of Public Health Importance in Cattle in Zimbabwe by 16S rRNA Gene Sequencing

Padya¹,

Chin'ombe²,

Magwenzi³

et al. 2015

TOMICROJ

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Mycobacterium species are naturally found in the environment as well as in domestic animals such as cattle. So far, more than 150 species of Mycobacterium, some of which are pathogenic, have been identified. Laboratory isolation, detection and identification of Mycobacterium species are therefore critical if human and animal infections are to be controlled. The objective of this study was to identify Mycobacterium species isolated in cattle in Zimbabwe using 16S ribosomal RNA gene amplification and sequencing. A total of 134 cow dung samples were collected throughout Zimbabwe and mycobacteria were isolated by culture. Only 49 culture isolates that were found to be acid-fast bacilli positive by Ziehl-Neelsen staining. The 16S rRNA gene was successfully amplified by PCR in 41 (84%) of the samples. There was no amplification in 8 (16%) of the samples. Out of the 41 samples that showed amplification, 26 (63%) had strong PCR bands and were selected for DNA sequencing. Analysis of the DNA sequences showed that 7 (27%) belonged to Mycobacterium neoaurum, 6 (23%) belonged to Mycobacterium fortuitum, 3 (12%) to Mycobacterium goodii, 2 (1%) to Mycobacterium arupense, 2 (1%) to Mycobacterium peregrinum or M. septicum and 1 isolate (0.04%) to Mycobacterium elephantis. There were 5 (19%) isolates that were non-mycobacteria and identified as Gordonia terrae, a close relative of Mycobacterium. The study therefore provided a molecular basis for detection and identification of Mycobacterium species in animals and humans.

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Occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk in the northwestern region of Paraná, Brazil

Cited by 23 publications

References 27 publications

Prevalence of bovine tuberculosis in buffaloes in Hyderabad and Tando Allahyar districts of Sindh, Pakistan

Prevalence of bovine tuberculosis in buffaloes in Hyderabad and Tando Allahyar districts of Sindh, Pakistan

Isolation of Mycobacterium bovis from Milk and Nasal Discharge Samples of Cattle from Hyderabad and Tando Allahyar Districts

Molecular Identification of Mycobacterium Species of Public Health Importance in Cattle in Zimbabwe by 16S rRNA Gene Sequencing

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