Prevalence of the paa gene (porcine attaching and effacing associated) in porcine enteropathogenic Escherichia coli (PEPEC) associated with postweaning diarrhea in south Brazil

Vidotto, Marilda Carlos; Florian, E.C. Teixeira; Ono, Mário Augusto

doi:10.1590/s1517-83822013000200030

Cited by 7 publications

(9 citation statements)

References 14 publications

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“…Results were 22% positive for the paa gene, which is associated with the presence of genes for other adhesins and toxins (F4, F18, F41, Paa, Sta, Stb, and LT). This demonstrates the importance of the paa gene as a virulence factor (VIDOTTO et al, 2013). The characteristics described above are listed in table 1.…”

Section: Etiologymentioning

confidence: 82%

Virulence factors of Escherichia coli in relation to the importance of vaccination in pigs

et al. 2016

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Enterotoxigenic Escherichia coli (ETEC) is the major cause of diarrhea in newborn and weaned pigs. Bacteria adhesion to the host cell is considered a specific phenomenon among fimbrial and non-fimbrial adhesins with their respective RESUMO Escherichia coli (E. coli) enterotoxigênica (ETEC) é considerada importante causa de diarreia em suínos neonatos e desmamados. A adesão da bactéria à célula do hospedeiro é considerada um fenômeno específico entre as adesinas fimbriais e não fimbriais com seus respectivos receptores nos enterócitos. Os distúrbios entéricos estão relacionados com as fímbrias F4 (K88), F5 (K99), F6 (987P),

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Section: Etiologymentioning

confidence: 82%

Virulence factors of Escherichia coli in relation to the importance of vaccination in pigs

et al. 2016

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“…The association of the paa gene of PEPEC with other ETEC virulence genes suggests its importance in the virulence of PEPEC (Leclerc et al, 2007;Vidotto et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…The paa-positive E. coli strain PEPEC was isolated from piglets with diarrhea in Paraná State (Vidotto et al, 2009(Vidotto et al, , 2013 Cloning and sequence analysis of the paa gene from PEPEC…”

Section: Strains Vectors and Chemicalsmentioning

confidence: 99%

“…The base sequences for the specific oligonucleotide primers used in this study were based on PAA PEPEC O45, forward: 5'-TCTTCTGCTGCTTATGCTGATATC-3ꞌ, and PAA PEPEC O45, reverse: 5'-TTACCAGCCATATTTTTTGAATGC-3', annealing at nucleotides 37 to 60 and 718 to 738 of the paa gene, respectively (Vidotto et al, 2013).…”

Section: Strains Vectors and Chemicalsmentioning

confidence: 99%

“…paa is mostly found with the enterotoxin gene estA and the autotransporter gene sepA, and it is carried on high-molecular weight plasmids (Leclerc et al, 2007). In Brazil, the paa gene has also been found in association with genes for other adhesins and toxins, and it has been found in 22% of E. coli strains isolated from piglets (Vidotto et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Cloning and expression of the porcine attaching and effacing-associated (paa) gene of enteropathogenic Escherichia coli

Pereira¹,

Florian²,

Ono³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Porcine enteropathogenic Escherichia coli (PEPEC)produce an outer membrane protein (intimin) called Paa (porcine attaching and effacing-associated), which is involved in the pathogenesis of E. coli in piglets with diarrhea. The paa gene of a PEPEC strain isolated in Paraná, Brazil, was amplified by polymerase chain reaction, sequenced, and cloned into the pTrcHisTOPO2 vector. The deduced amino acid sequence encoded by the paa gene of PEPEC from Paraná, Brazil, showed 99% homology to the sequences from other PEPEC strains. In this study, the overexpression of recombinant Paa (rPaa) using alternative induction strategies was attempted. The auto-induction protocol showed excellent results for rPaa protein production with 0.4% (w/v) lactose. The rPaa protein is insoluble and was purified with Triton X-100 wash as a total antigen. This method produced a relatively high yield of rPaa. rPaa was recognized by serum from pigs immunized with the PEPEC strain. These results suggest that rPaa could be included in the development of a vaccine against swine colibacillosis.

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Characterisation of ESBL/AmpC-Producing Enterobacteriaceae isolated from poultry farms in Peninsular Malaysia

Tan

Pan

Agustie

et al. 2022

Letters in Applied Microbiology

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Extended-spectrum beta-lactamases (ESBLs) and AmpC beta-lactamases (AmpCs)-producing Enterobacteriaceae have been increasingly reported and imposing significant threat to public. Livestock production industry might be the important source for clinically important ESBL-producing Enterobacteriaceae. This study aims to investigate the resistance profile, phenotypic ESBL production, beta-lactamase genes, virulence factors, and plasmid replicon types among 59 Enterobacteriaceae strains isolated from poultry faecal samples in Malaysia’s commercial poultry farm. There were 38.7% and 32.3% of Escherichia coli resistant to cefotaxime and cefoxitin, respectively, while Klebsiellaspp. demonstrated resistance rate of 52.6% to both mentioned antimicrobials. Majority of the E. coli isolates carried blaTEM and blaCMY-2 group. blaSHV was the most prevalent gene detected in Klebsiellaspp., followed by blaDHA and blaTEM. Resistance to extended spectrum cephalosporin in our isolates was primarily mediated by plasmid mediated AmpC beta-lactamase such as CMY-2 group and DHA enzyme. The CTX-M genes were found in two ESBL-producing E. coli. IncF, IncI1, and IncN plasmids were most frequently detected in E. coli and Klebsiellaspp. The virulence factor, including EAST1 and pAA were identified at low frequency. This study highlights the poultry as a reservoir of resistance and virulence determinants and prevalence of plasmids in Enterobacteriaceae might drive their dissemination.

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Prevalence of the paa gene (porcine attaching and effacing associated) in porcine enteropathogenic Escherichia coli (PEPEC) associated with postweaning diarrhea in south Brazil

Cited by 7 publications

References 14 publications

Virulence factors of Escherichia coli in relation to the importance of vaccination in pigs

Virulence factors of Escherichia coli in relation to the importance of vaccination in pigs

Cloning and expression of the porcine attaching and effacing-associated (paa) gene of enteropathogenic Escherichia coli

Characterisation of ESBL/AmpC-Producing Enterobacteriaceae isolated from poultry farms in Peninsular Malaysia

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