'Synthetic lipase' production from a newly isolated Sporidiobolus pararoseus strain by submerged fermentation

Abstract

The lipase produced by a newly isolate Sporidiobolus pararoseus strain has potential catalysis ability for esterification reactions. In order to improve its synthetic activity, this work aimed at optimizing 'synthetic lipase' production by submerged fermentation of a conventional media based on peptone, yeast extract, NaCl and olive oil using experimental design technique. According to the results obtained in the first experimental design (2 ), yeast extract and NaCl concentrations were tested to further optimization by 4-1 response surface methodology. The maximum 'synthetic lipase' activity obtained was 26.9 U/mL in the optimized media (5.0, 6.8, 7.0 and 1.0% (wt/v) of peptone, yeast extract, NaCl and olive oil, respectively), representing a 6.36-fold increase compared to the initial medium. The time course of 'synthetic lipase' production in the optimized condition was evaluated in terms of synthetic activity, protease activity, biomass and total carbon and the maximum synthetic activity was observed during the stationary phase of growth.

Production of microbial carotenoids by Rhodotorula lactosa isolated in the Brazilian tropical savanna / Produção de carotenoides microbianos por Rhodotorula lactosa isolada na savana tropical brasileira

“…Carotenoid-producing yeast strains, capable of using co-products for growth, and isolated from soil, leaves, tree bark and flower petals found in the savanna biome (latitude -15.853641 and longitude -52.264068), were transferred to 250 mL Erlenmeyer flask containing 50 mL Yeast Malt -(YM) isolation medium (3 g/L yeast extract, 3 g/L malt Brazilian Journal of Development, Curitiba, v.7, n.9, p. 89302-89322 sep. 2021 extract, 5 g/L peptone, 10 g/L glucose) and incubated at 25 °C and shaken at 150 rpm for 48 h to promote microbial enrichment. They were then inoculated onto YM-agar in Petri dishes and incubated at 25 °C for 120 h [13].…”

Carotenoid-Producing Yeasts: Identification and Characteristics of Environmental Isolates with a Valuable Extracellular Enzymatic Activity

“…Several yeasts have been described as lipase producers, and among them species of the genus Candida are the most commercially used ones [31]. However, lipase-producing basidiomycetes species have also been isolated and characterized [2,20,[32][33][34][35]. In accordance with recent reports, in this study most of the strains were able to synthesize lipases and gave positive results in the plate tests ( Table 2 and Figure S1), w hereas, a proteolytic activity was only demonstrated for Cystofilobasidium macerans WUT145 ( Figure S2).…”

Isolation of Lipase-Producing Yeasts from Industrial Oily Residues in Different Culture Media

“…The isolation of different strains is the key for discovering novel lipases of interest to industry in general, and in particular of interest to the food industry (Romdhane et al, 2010). Studies indicate the isolation of lipolytic yeasts from various samples, for example, flowers, fruits, soil, leaf surface (Bussamara et al, 2010;Goldbeck and Filho, 2013;Ramesh et al, 2014), butter cheese (Souza et al, 2015), soybean meal (Smaniotto et al, 2012), wastewater and water samples (Odeyemi et al, 2013), among other sources.…”