Evaluation of the composition of culture medium for yeast biomass production using raw glycerol from biodiesel synthesis

Abstract

The work herewith investigated the production of yeast biomass as a source of protein, using Yarrowia lipolytica NRRL YB-423 and raw glycerol from biodiesel synthesis as the main carbon source. A significant influence of glycerol concentration, initial pH and yeast extract concentration on biomass and protein content was observed according to the 2v5-1 fractional design. These factors were further evaluated using a central composite design and response surface methodology, and an empirical model for protein content was established and validated. The biomass of Yarrowia lipolytica NRRL YB-423 reached 19.5 ± 1.0 g/L in shaken flasks cultivation, with a protein content of 20.1 ± 0.6% (w/w).

Carotenoids from Phaffia rhodozyma: Antioxidant activity and stability of extracts

“…For reactivation of stock cultures, transfers to tubes containing the same medium were made and the tubes were incubated for 48 h at 25°C. They were scraped with 10 mL of 0.1% (w/v) peptone diluent for each tube and transferred to 500 mL Erlenmeyer flask (Santos et al, 2012) containing YM medium (3 g/L yeast extract; 3 g/L malt extract; 5 g/L peptone; and 10 g/L glucose) and incubated under the same conditions.…”

Optimization of agroindustrial medium for the production of carotenoids by wild yeast Sporidiobolus pararoseus

“…The composition of the production medium using S. salmonicolor (Valduga et al, 2009(Valduga et al, , 2014 and for S. pararoseus (Cabral et al, 2011). This methodology is also applied to obtain other bioproducts, such as biomass (Santos et al, 2012), galacto-oligosaccharides (Lisboa et al, 2012a;Lisboa et al, 2012b), dairy drinks (Martins et al, 2011;Martins et al, 2012;Burkert et al, 2012), enzymes (Maldonado et al, 2012;Campello et al, 2012;Alves et al, 2010), synthesis of natural flavors (Anschau et al, 2011;Aragão et al, 2011), phycocyanin (Moraes et al, 2010) and rhamnolipids (Rosa et al, 2010).…”

Single cell protein production by Wickerhamomyces anomalus CCC32 using crude glycerol

“…Kurcz et al (2018) also reported a significant influence of glycerol concentration on biomass and crude protein production by Candida utilis. Dos Santos et al (2012) observed the concentration of crude glycerol had significant effect only on the biomass production by Yarrowia lipolytica NRRL YB-423, but did not have a significant effect for crude protein production.…”