Carotenoids from Phaffia rhodozyma: Antioxidant activity and stability of extracts

Cipolatti, Eliane Pereira; Bulsing, Bruna A.; Santos, Carolina dos; Burkert, Carlos André Veiga; Furlong, Eliana Badiale; Burkert, Janaína Fernandes de Medeiros

doi:10.5897/ajb2015.14682

Cited by 29 publications

(8 citation statements)

References 26 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The major compound found was (all-E) -β-carotene representing 76% of the total carotenoids (634 µg/g), followed by (13Z)-β-carotene (11.1%), (9Z)-β-carotene (7.4%), and γ-carotene (5.5%). The results obtained in the present study corroborate with Cipolatti et al [27] in the evaluation of the carotenogenic profile of the extract obtained from P. rhodozyma that quantified the β-carotene as the major compound.…”

Section: Discussionsupporting

confidence: 91%

Fed-batch carotenoid production by Phaffia rhodozyma Y-17268 using agroindustrial substrates

2020

Biointerface Res Appl Chem

View full text Add to dashboard Cite

This work aim the carotenoid bioproduction by the yeast Phaffia rhodozyma Y-17268 in a fed-batch bioreactor with different low-cost agroindustrial substrates (crude glycerol, corn steep liquor, and rice parboiling water). The maximum concentration of total carotenoid and cell productivity were 4118 µg/L (835 µg/g) and 0.05 g/L. h, respectively, with a feed volume of 75 mL every 12 h. The medium were composed of 100 g/L crude glycerol, 100 g/L corn steep liquor, and 20 g/L rice parboiling water at 25ºC, pHinitial 4.0, agitation rate of 250 rpm, aeration rate of 1.5 vvm and 96 h of bioproduction. 0.188 h-1 of maximum specific growth speeds (μmax) was obtained for the major carotenoid - (all-E)-β-carotene (75.9%). Thus, the yeast P. rhodozyma produced in a fed-batch bioreactor demonstrated a great potential to produce the β-carotene.

show abstract

Section: Discussionsupporting

confidence: 91%

Fed-batch carotenoid production by Phaffia rhodozyma Y-17268 using agroindustrial substrates

2020

Biointerface Res Appl Chem

View full text Add to dashboard Cite

show abstract

“…This was transferred to test tubes containing 9 mL YM broth and maintained at 25 °C for 48 h. The cultures were then stirred on a vortex and transferred to 250 mL conical flasks containing 50 mL YM broth, previously sterilized at 121 °C for 15 min. These cultures were incubated at 150 rpm and 25 °C, either for 48 h or the time required for the cell count to reach 10 7 cells/mL, counting in a Neubauer chamber (Cipolatti et al, 2015).…”

Section: Inoculummentioning

confidence: 99%

“…Total carotenoid recovery was carried out according to the methodology described by Michelon et al (2012) and Cipolatti et al (2015). The biomass was centrifuged at 3439 × g for 10 min, transferred to a Petri dish, dried in a freeze-dryer for 48 h, macerated in a mortar and standardized using a Tyler nº 115 sieve.…”

Section: Recovery Of Total Carotenoidsmentioning

confidence: 99%

Evaluation of the process conditions for the production of microbial carotenoids by the recently isolated Rhodotorula mucilaginosa URM 7409

Machado

Silva

Lago-Vanzela

et al. 2019

Braz. J. Food Technol.

View full text Add to dashboard Cite

This study aimed to improve the physical and nutritional process conditions for the production of carotenoids by the newly isolated Rhodotorula mucilaginosa, a red basidiomycete yeast. The carotenoid bioproduction was improved using an experimental design technique, changing the process characteristics of agitation (130 rpm to 230 rpm) and temperature (25 °C to 35 °C) using seven experiments, followed by a 25-1 fractional design to determine the relevant factors that constitute the culture medium (glucose, malt extract, yeast extract, peptone and initial pH). A complete second order experimental design was then carried out to optimize the composition of the culture medium, the variables being yeast extract (0.5 to 3.5 g/L), peptone (1 to 5 g/L) and the initial pH (5.5 to 7.5), with 17 experiments. The maximum carotenoid production was 4164.45 μg/L (252.99 μg/g), obtained in 144 h in YM (yeast malt) medium with 30 g/L glucose, 10 g/L malt extract, 2 g/L yeast extract, 3 g/L peptone, an initial pH 6, 130 rpm and 25 °C, demonstrating the potential of this yeast as a source of bio-pigments. In this work, the nitrogen sources were the factors that most influenced the intracellular accumulation of carotenoids. The yeast R. mucilaginosa presented high production at a bench level and may be promising for commercial production.

show abstract

“…The total carotenoids (TC) were determined according to the methodology of Fonseca et al (2011), modified by Cipolatti et al (2015). For this purpose, 2 mL of dimethylsulfoxide (55 °C) was mixed with 0.05 g dry biomass (35 °C for 48 h), ground, sieved (Tyler 115) and frozen (-18 °C for 48 h).…”

Section: Carotenoid Concentrationmentioning

confidence: 99%

“…Carotenoids are natural pigments widely used in the food, pharmaceutical, cosmetic, and animal feed industries due to their biological properties, such as antioxidant activity (Valduga et al, 2009). Greater consumer awareness with regard to health and functional foods has triggered an increase in the demand for carotenoids, highlighting the biotechnological production due to the proven biological activity of these biomolecules (Cipolatti et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

SIMULTANEOUS LIPID AND CAROTENOID PRODUCTION BY STEPWISE FED-BATCH CULTIVATION OF Rhodotorula mucilaginosa WITH CRUDE GLYCEROL

Pereira

Silveira

Burkert

et al. 2019

Braz. J. Chem. Eng.

View full text Add to dashboard Cite

In the present study, an innovative cultivation strategy of Rhodotorula mucilaginosa CCT 7688 to accumulate both lipids and high added value carotenoids was proposed, with the potential to contribute to the economic feasibility of microbial oils in comparison with vegetable oils. The stepwise fed-batch cultivation with crude glycerol feeding at 24 h, 96 h, and 144 h, with the addition of magnesium to the initial culture medium, resulted in a lipid content of 51.0 ± 0.3 % CDW, biomass concentration of 21.00 ± 0.48 g L-1 , total lipid production of 10.72 ± 0.18 g L-1 , lipid productivity of 0.037 ± 0.001 g L-1 h-1 , volumetric carotenoid production of 2843.2 ± 282.0 μg L-1 , and carotenoid productivity of 9.87 ± 0.98 μg L-1 h-1. These values represent a 6-fold increase in lipid content, a 19-fold increase in lipid production, and a 2-fold increase in carotenoid production compared to batch cultivation.

show abstract

Carotenoids from Phaffia rhodozyma: Antioxidant activity and stability of extracts

Cited by 29 publications

References 26 publications

Fed-batch carotenoid production by Phaffia rhodozyma Y-17268 using agroindustrial substrates

Fed-batch carotenoid production by Phaffia rhodozyma Y-17268 using agroindustrial substrates

Evaluation of the process conditions for the production of microbial carotenoids by the recently isolated Rhodotorula mucilaginosa URM 7409

SIMULTANEOUS LIPID AND CAROTENOID PRODUCTION BY STEPWISE FED-BATCH CULTIVATION OF Rhodotorula mucilaginosa WITH CRUDE GLYCEROL

Contact Info

Product

Resources

About