Brucella canis infection in dogs attended in veterinary clinics from patos, Paraíba state, Brazil

Fernandes, Annielle Regina Fonseca; Azevêdo, Sérgio Santos de; Piatti, Rosa Maria; Pinheiro, Eliana Scarcelli; Genovez, Margareth Élide; Azevedo, Adílio Santos de; Batista, Carolina de Sousa Américo; Alves, Clebert José

doi:10.1590/s1517-83822011000400023

Cited by 14 publications

(10 citation statements)

References 4 publications

(4 reference statements)

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“…Nota-se que com a utilização das amostras de suabe do trato reprodutivo, a frequência de amplificações positivas de DNA passou de 3,7% (3/80), utilizandose apenas amostras de sangue, para 7,5% (6/80), no entanto, não foi observada diferença significativa (p = 0,071). Essa diferença entre as amostras na detecção molecular do agente, embora não identificada estatisticamente, pode ser explicada pelo fato de a bacteremia na brucelose canina, apesar de prolongada, passar a ser intermitente e eventualmente diminuir em infecções crônicas, de modo que o agente passa a ser recuperado a partir de outros sítios de infecção (FERNANDES et al, 2011).…”

Section: Resultsunclassified

Detecção de DNA de Brucella spp. em amostras de sangue e de suabe vaginal ou prepucial de cães do município de Natal, Rio Grande do Norte, Brasil

Fernandes

Kim

Dantas

et al. 2013

Braz. J. Vet. Res. Anim. Sci.

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Objetivou-se com este trabalho detectar o DNA de Brucella spp. em amostras de sangue e de suabe vaginal ou prepucial de 80 cães sorologicamente positivos para brucelose pela prova de imunodifusão em gel de ágar (IDGA), no município de Natal, estado do Rio Grande do Norte, Brasil. Amostras de sangue total foram colhidas com anticoagulante (citrato de sódio) juntamente com amostras de suabe vaginal e prepucial , para extração de DNA e posterior realização da reação em cadeia da polimerase (PCR) empregando-se os primers ITS66 e ITS279. O DNA de Brucella spp. foi amplificado em seis animais, sendo um animal em ambas as amostras, dois cães em amostras de sangue e três em amostras de suabe do trato reprodutivo. Concluiu-se que a infecção por Brucella spp. está presente em cães no município de Natal, e que a detecção de DNA do agente em amostras de suabe do trato reprodutivo podem ser utilizadas como ferramenta suplementar no diagnóstico de brucelose canina.

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Section: Resultsunclassified

Detecção de DNA de Brucella spp. em amostras de sangue e de suabe vaginal ou prepucial de cães do município de Natal, Rio Grande do Norte, Brasil

Fernandes

Kim

Dantas

et al. 2013

Braz. J. Vet. Res. Anim. Sci.

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“…Canine brucellosis caused by Brucella canis is a neglected zoonosis worldwide distributed (Holst et al., ), being considered the main cause of reproductive failures in dogs, and economic losses to breeding kennels (Gyuranecz et al., ; Hofer et al., ; Holst et al., ; Jones & Emerson, ; Pickerill & Carmichael, ). The infection has been evidenced in Brazil mainly through serological surveys with frequencies varying from 0.8% to 44.5% (Cavalcanti et al., ; Dorneles et al., ; Dreer et al., ; Fernandes et al., , ; Malek dos Reis, Hoffmann, Santos, Turri, & Oriani, ; Mascolli et al., ; Silva et al., ; Vasconcelos et al., ). However, few reports of the infection in dogs were based on bacteriological evidence (Keid, Soares, Morais, Richtzenhain, & Vasconcellos, ; Larsson & Costa, ; Mascolli et al., ).…”

Section: Introductionmentioning

confidence: 99%

Brucella canisinfection in dogs from commercial breeding kennels in Brazil

Keid

Chiebáo

Batinga

et al. 2017

Transbound Emerg Dis

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Canine brucellosis caused by Brucella canis is a neglected zoonosis worldwide and is a leading cause of reproductive failure in dogs, often causing substantial economic losses in breeding kennels. This study aimed to investigate the occurrence of B. canis infection in dogs of commercial breeding kennels located in São Paulo State, Brazil. A total of 753 dogs (183 males and 570 females) from 38 commercial kennels were clinically examined, and blood samples were collected for brucellosis diagnosis through blood culture. The association between clinical manifestations suggestive of brucellosis and positive results through blood culture was determined. Of the 753 dogs tested, 166 (22.0%) had at least one clinical sign suggestive of brucellosis and 158 (20.9%) had positive blood cultures. Seventy-two dogs had positive blood culture and had at least one clinical sign suggestive of brucellosis, while 91 dogs showed at least one clinical manifestation suggestive of brucellosis although blood culture was negative. Of the 38 kennels, 16 (42.1%) had at least one positive dog. The prevalence of infection in each kennel varied from 3.8% to 62.6%. Abortion/stillbirth, failure to conceive and enlargement of lymph nodes were significantly associated with brucellosis in female. No association of clinical signs and positive results in blood culture was observed in males. None of the kennels has been carrying out programmes to control brucellosis, and the sale of infected dogs was considered a common practice yielding risks to the public health, in view of the zoonotic potential of the infection.

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“…Conversely, the serological surveys for B. abortus are rare. In the state of Paraíba, a few studies investigating the prevalence of B. canis in dogs have been conducted (ALVES et al, 2003;VASCONCELOS et al, 2008;FERNANDES et al, 2011), but only one study was conducted on B. abortus (AZEVEDO et al, 2003). In addition, there are no studies that show the occurrence of canine brucellosis in the Brejo Paraibano microregion, a place which has characteristics that may influence the occurrence of infection such as the presence of fragments of the Atlantic Forest, high rainfall, and a wide variety of fauna that may serve as natural reservoirs (ZHELUDKOV & TSIRELSON, 2010).…”

Section: Introductionmentioning

confidence: 99%

Zoonotic smooth and rough Brucella in dogs: seroprevalence and associated factors in an Atlantic Rainforest area of the state of Paraíba, Northeastern Brazil

et al. 2021

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Canine brucellosisis an infectious disease caused by bacteria of the genus Brucella, with world wide distribution and zoonotic impact, and in humans and animals is a neglected disease. In the present study, the sero prevalence of B. canis and B. abortus were determined in a probabilistic sample of housed dogs from the Atlantic Rainforest area of the state of Paraíba, Brazil, and the factors associated with sero positivity. A total of 386 dogs over three months of age were used. For the search for anti-B.canis antibodies the agar gel immune diffusion test (IDGA) was used as a screening and IDGA+2ME as confirmatory test, and to search for anti-B. abortus antibodies the Rose Bengal test (RBT) test was used. Apparent and real prevalences were calculated, and robust Poisson regression was used to identify factors associated with prevalence. The real prevalence fB. Canis was 12.6% and of B. abortus was 22.8%. The factors associated with sero positivity for B. canis were age greater than 10 years (prevalence ratio; PR = 6.38; P = 0.024) and dogs reared in they ard (PR = 5.20; P = 0.035) and for B. abortus was no treplacement of water of animals everyday (PR = 1.48; P = 0.033). It can be concluded that the prevalence of B. canis and B. Abortus in the region is high, which warns to the adopting of control and prevention measures, as well as greater care in the management of animals, especially for elderly dogs.

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Brucella canis infection in dogs attended in veterinary clinics from patos, Paraíba state, Brazil

Cited by 14 publications

References 4 publications

Detecção de DNA de Brucella spp. em amostras de sangue e de suabe vaginal ou prepucial de cães do município de Natal, Rio Grande do Norte, Brasil

Detecção de DNA de Brucella spp. em amostras de sangue e de suabe vaginal ou prepucial de cães do município de Natal, Rio Grande do Norte, Brasil

Brucella canisinfection in dogs from commercial breeding kennels in Brazil

Zoonotic smooth and rough Brucella in dogs: seroprevalence and associated factors in an Atlantic Rainforest area of the state of Paraíba, Northeastern Brazil

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