Canine brucellosis caused by Brucella canis is a neglected zoonosis worldwide and is a leading cause of reproductive failure in dogs, often causing substantial economic losses in breeding kennels. This study aimed to investigate the occurrence of B. canis infection in dogs of commercial breeding kennels located in São Paulo State, Brazil. A total of 753 dogs (183 males and 570 females) from 38 commercial kennels were clinically examined, and blood samples were collected for brucellosis diagnosis through blood culture. The association between clinical manifestations suggestive of brucellosis and positive results through blood culture was determined. Of the 753 dogs tested, 166 (22.0%) had at least one clinical sign suggestive of brucellosis and 158 (20.9%) had positive blood cultures. Seventy-two dogs had positive blood culture and had at least one clinical sign suggestive of brucellosis, while 91 dogs showed at least one clinical manifestation suggestive of brucellosis although blood culture was negative. Of the 38 kennels, 16 (42.1%) had at least one positive dog. The prevalence of infection in each kennel varied from 3.8% to 62.6%. Abortion/stillbirth, failure to conceive and enlargement of lymph nodes were significantly associated with brucellosis in female. No association of clinical signs and positive results in blood culture was observed in males. None of the kennels has been carrying out programmes to control brucellosis, and the sale of infected dogs was considered a common practice yielding risks to the public health, in view of the zoonotic potential of the infection.
It is suggested that Bovine kobuvirus (BKV) is involved in the etiology of gastroenteric diseases especially among calves; however, this association remains unknown. This study evaluated 216 fecal samples from cattle with and without diarrhea symptoms obtained from different regions of Brazil. A 216 bp fragment of the BKV 3D gene was amplified by RT-PCR in 14.4 % (31/216) of the studied samples, and 17 samples were subjected to nucleotide sequencing. All positive samples were obtained from animals aged less than 5 months, and most of animals presented diarrhea (p < 0.05). Phylogenetic analyses showed that the obtained sequences were grouped within the genogroup 2 of BKV forming subclades specific for each Brazilian municipality sampled. In addition, the alignment of the sequences revealed differences of nucleotides between sequences from different locations. Our results indicate for the first time that there is a regional genotypic differentiation of BKV in Brazil.
: We surveyed 13 carcasses of marine mammals (12 Trichechus manatus and one Stenella clymene) that had stranded in northeastern Brazil during 1990-2013 for infectious diseases by screening tissues from the collection of the Brazilian National Center of Research and Conservation of Aquatic Mammal, Chico Mendes Institute for Biodiversity Conservation. Brucella spp. and Mycobacterium spp. were investigated by culturing and PCR of tissue samples, whereas Sarcocystidae parasites, Leptospira spp., and Morbillivirus were surveyed for using specific PCR assays. Brucella spp. and Mycobacterium spp. were not isolated through microbiologic culturing, and all animals were negative for detection of Sarcocystidae parasites, Leptospira spp., Mycobacterium spp., and Morbillivirus by PCR assays. All manatees were negative for Brucella spp. infection, but Brucella ceti was detected in the brain tissue of an S. clymene calf by using a PCR assay.
Certificamos que o Projeto intitulado "Diagnóstico molecular comparativo da brucelose canina pela aplicação das técnicas de reação em cadeia pela polimerase (PCR) e amplificação isotérmica do DNA mediada por loop (LAMP) ", protocolado sob o CEUA nº 9290230216, sob a responsabilidade de Lara Borges Keid e equipe; Maria Cryskely Agra Batinga-que envolve a produção, manutenção e/ou utilização de animais pertencentes ao filo Chordata, subfilo Vertebrata (exceto o homem), para fins de pesquisa científica (ou ensino)-encontra-se de acordo com os preceitos
A Brucella canis é uma bactéria intracelular facultativa responsável pela brucelose nos cães, uma doença infectocontagiosa de caráter crônico e zoonótico, endêmica no Brasil e associada a problemas reprodutivos e comprometimento dos sistemas linfoide e articular. A infecção é comumente descrita em canis de reprodutores, sendo de difícil tratamento e controle. Este trabalho relata um surto de brucelose em um canil comercial localizado em Guarulhos (São Paulo), composto por 32 cães (4 machos e 28 fêmeas) de 5 raças distintas, no qual episódios de abortamento foram relatados desde outubro de 2015. A suspeita de brucelose foi aventada apenas um ano e meio após a ocorrência do primeiro episódio de abortamento, sendo realizados anamnese, exames clínicos e diagnósticos sorológico e bacteriológico em todos os cães do plantel. Dos 32 cães, 28 apresentaram pelo menos um sinal clínico compatível com brucelose, sendo o aumento de linfonodos e o abortamento os sinais mais frequentemente observados. Vinte e seis animais apresentaram resultado positivo em pelo menos um dos testes laboratoriais usados para o diagnóstico, indicando prevalência de 81,25% na criação. No diagnóstico microbiológico, houve isolamento de B. canis em amostras de sangue de 22 cães, em amostras de swab vaginal de 9 fêmeas e em sêmen ou swab prepucial de 2 machos. Vinte e três cães apresentaram resultados positivos no sorodiagnóstico. Quando introduzida em criações caninas confinadas, a infecção pode se disseminar rapidamente, levando a grandes perdas reprodutivas. A elevada prevalência observada no canil pode ser relacionada à demora para a investigação da infecção após a ocorrência do primeiro episódio de abortamento, bem como à existência de condições de manejo favoráveis à introdução e rápida disseminação da infecção. Assim, o presente relato alerta para a importância da imediata investigação de problemas reprodutivos em cães por meio de testes laboratoriais para diagnóstico da brucelose canina.
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