Identification, isolation and optimization of antifungal metabolites from the Streptomyces Malachitofuscus ctf9

Abstract

An indigenous Streptomyces isolate CTF9, exhibiting promising antifungal activity against Mucor mieheiand Candida albicans in pre-screening studies, was investigated by cultivation in a 50-L fermenter and by subsequent isolation, purification, and structure elucidation of the active metabolites. Based on the morphological, biochemical, and physiological characterization, as well as the 16S rRNA gene sequence, the isolate CTF9 was identified as Streptomyces malachitofuscus. Using a series of chromatographic techniques, two pure compounds were isolated from the obtained extracts after the fermentation of the isolate CTF9. The isolated compounds were identified as phenylacetic acid and indolyl-3-lactic acid by mass spectrometry (MS) and NMR analysis. The culture optimization studies revealed that the isolate CTF9 can use a variety of low-cost carbon and nitrogen sources to generate the maximum quantity of industrially important metabolites at an elevated temperature of 35°C and at a pH 7.8.

Antimicrobial, Antioxidant and Cytotoxic Activity of MarineStreptomyces parvulus VITJS11 Crude Extract

“…The Streptomyces sp. isolated from saline farm-lands showed both antibacterial and antifungal activity (Sajid et al 2009). The antioxidant activity of the VITJS11 crude extract showed maximum activity with 85% inhibition at 5.0 mg/mL concentration and its activity remained highly significant with the reference used (Fig.4).…”

Biodiversity of Secondary Metabolites Compounds Isolated from Phylum Actinobacteria and Its Therapeutic Applications

“…strains isolated from the Japan Sea's sediment, Goodfellow and Fiedler [51] identified atrop-abyssomicin C and proximicins A, B and C. It inhibits the formation of p-aminobenzoate during tetrahydrofolate synthesis in Gram-positive bacteria with methicillin-resistant Staphylococcus aureus (MRSA). The Streptomyces malachitofuscus has anti-fungal action against Candida albicans and Mucor miehei, as Sajid et al [52] reported. The spoxazomicins was extracted from Streptosporangium oxazolinicum sp.…”

Antibacterial and anticancer activity of marineStreptomyces parvus: optimization and application

“…The genus Streptomyces seems to provide a wide variety of new antibiotics more than any other genus; hence, it is of great importance for both industrial applications and human health care. [40] Optimization of culture conditions…”