Production of laccase by Pynoporus sanguineus using 2,5 - Xylidine and ethanol

Valeriano, Viviane S.; Silva, Anna Maria F.; Santiago, Mariângela Fontes; García, Telma Alves

doi:10.1590/s1517-83822009000400009

Cited by 27 publications

(18 citation statements)

References 17 publications

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“…Ten 5 mm diameter mycelium discs were inoculated in 50 mL of malt extract broth (Merck, Darmastadt, Germany) plus substrate (4, 19, 28). Cultures were grown in conical flasks (250 ml) with shaking (150 rpm) at 30°C for 7 days (16).…”

Section: Methodsmentioning

confidence: 99%

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Production and characterization of tyrosinase activity in Pycnoporus sanguineus CCT-4518 crude extract

Duarte

Tiba

Santiago

et al. 2012

Braz. J. Microbiol.

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Tyrosinase is an enzyme of industrial interest. The production and characterization of tyrosinase from P. sanguineus CCT-4518 were investigated. The selection of inductors, luminosity influence, inoculum size and type of culture medium on the production of tyrosinase and the effect of inhibitors on enzyme activity were performed. Optimum conditions for intracellular tyrosinase production was observed after 2 days using 0.15% L-tyrosine as inducer, in the presence of light, with inoculum size of 10 mycelium discs, using 2% malt extract broth medium, incubated at 30°C, and constant agitation of 150 rpm. Tyrosinase activity was completely inhibited by the addition of 6 mM salicylhydroxamic acid or phenylthiourea, however an inhibition of 4.15% was recorded by the addition of 0.1 mM sodium azide. No inhibition could be detected in case of 0.1 mM phenyl methanesulfonyl fluoride addition. Optimal conditions for intracellular tyrosinase activity using L-dopa as substrate were observed at pH 6.6 and 45°C. Thermal stability studies indicated that the enzyme is stable at 45°C for 15 minutes. Higher temperatures decreased tyrosinase activity. Enzyme production was confirmed by non-denaturing polyacrylamide gel electrophoresis and the protein profile was investigated by denaturing polyacrylamide gel electrophoresis.

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Section: Methodsmentioning

confidence: 99%

“…Statistical analysis was carried out according to Valeriano et al (28). Assays were carried out in triplicate, and standard deviation did not exceed 10% of the average values.…”

Section: Methodsmentioning

confidence: 99%

Production and characterization of tyrosinase activity in Pycnoporus sanguineus CCT-4518 crude extract

Duarte

Tiba

Santiago

et al. 2012

Braz. J. Microbiol.

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“…Dantán-González et al (2008) reported a 68-kDa protein purified from submerged cultures of Pycnoporus sanguineus; this protein showed laccase activity towards 2,2´-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), guaiacol, syringaldazine, and o-dianisidine. Valeriano et al (2009) reported an increase in Pycnoporus sanguineus laccase activity in the presence of xylidine and ethanol as inducers and observed the maximum laccase activity of 2019 U/L and 1035 U/L, with 200 mg/L of 2,5-xylidine and 50 g/L of ethanol, respectively. Laccases from Pycnoporus cinnabarinus are involved in cinnabarinic acid production with antibacterial activity (Eggert 1997).…”

Section: Resultsmentioning

confidence: 99%

Enzymatic, Antioxidant, Antimicrobial, and Insecticidal Activities of Pleurotus pulmonarius and Pycnoporus cinnabarinus Grown Separately in an Airlift Reactor

Díaz‐Godínez¹,

Téllez‐Téllez²,

Rodríguez³

et al. 2016

BioResources

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Crude extract samples of Pleurotus pulmonarius and Pycnoporus cinnabarinus were taken during growth in liquid broth in an airlift reactor. Growth was monitored indirectly by sugar consumption and pH profile. During growth Pleurotus pulmonarius consumed glucose more slowly than Pycnoporus cinnabarinus, reaching a final pH of 8.0. In contrast, Pycnoporus cinnabarinus started consuming glucose faster from the beginning to the end with a pH of 3.6, suggesting the production of different metabolites while they grow in the same culture broth. Additionally, antioxidant activity, polyphenol and flavonoid contents, as well as laccase and hydrolase activities were quantified in the culture extracts during the fermentation. Pleurotus pulmonarius showed higher antioxidant activity than Pycnoporus cinnabarinus. Both fungi have a very low polyphenol and flavonoid content. Values of amylase and pectinase activities were similar in crude extracts of both fungi; however, cellulase, xylanase, invertase, and laccase activities showed higher levels in crude extract of Pleurotus pulmonarius. Antimicrobial and insecticidal activities were also evaluated in each crude extract. In fact, Pycnoporus cinnabarinus presented a very strong bacteriostatic and bactericidal effect against Escherichia coli and Staphylococcus aureus and reliably killed Diatraea magnifactella larvae, while Pleurotus pulmonarius did not showed any negative effect on the growth of these bacteria or larvae.

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“…A notable decrease in substrate phenols has also been reported for the first stages of growth in Pleurotus sp., concurrent with high Mn peroxidase activity. The activity of Mn peroxidase and laccase enzymes is linked to a response of the mushroom to oxidize chemical compounds and decrease their toxic effects (54, 55). Zheng and Shetty (58) have also mentioned that L. edodes has high levels of extracellular enzymes, such as ß-glucosidases, that are capable of hydrolyzing phenolic compounds.…”

Section: Resultsmentioning

confidence: 99%

Quantitative changes in the biochemical composition of lignocellulosic residues during the vegetative growth of Lentinula edodes

Gaitán-Hernández¹,

Esqueda²,

Gutiérrez³

et al. 2011

Braz. J. Microbiol.

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The chemical changes in barley-straw (BS), wheat-straw (WS) and vineyard-pruning (VP) substrates were determined during colonization of Lentinula edodes mycelia (during primordium development) in solid state fermentation. Primordia appeared 39-50 days after inoculation. VP appeared to promote early sporophore initiation. The concentration of hemicellulose in BS and VP decreased gradually from 25.5% to 15.6% and from 15.8% to 12.3%, respectively. However in WS, hemicellulose decreased from 27.2% to 9.5%. Lignin broke down continuously in BS and WS, with 31.8% and 34.4% degradation, respectively; higher than that of cellulose. During the pinning stage, the C:N ratio decreased in VP and BS, but not in WS. On all substrates the phenols decreased notably throughout the first week of mycelial growth. The time elapsed (days) to pinning was positively correlated with cellulose content (r=0.89), total sugar (r=0.85) and inversely correlated to lignin (r=−1.00) and phenol content (r=−0.55).

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Production of laccase by Pynoporus sanguineus using 2,5 - Xylidine and ethanol

Cited by 27 publications

References 17 publications

Production and characterization of tyrosinase activity in Pycnoporus sanguineus CCT-4518 crude extract

Production and characterization of tyrosinase activity in Pycnoporus sanguineus CCT-4518 crude extract

Enzymatic, Antioxidant, Antimicrobial, and Insecticidal Activities of Pleurotus pulmonarius and Pycnoporus cinnabarinus Grown Separately in an Airlift Reactor

Quantitative changes in the biochemical composition of lignocellulosic residues during the vegetative growth of Lentinula edodes

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