Utilization of agroindustrial residues for lipase production by solid-state fermentation

Abstract

The aim of this work was to produce lipases by solid-state fermentation (SSF) using, as substrate, agroindustrial residue supplemented with by-products from corn oil refining process or olive oil. For a group of ten fungi strains selected in the first steps, the lipase activity obtained by SSF varied from 7.7 to 58.6 U/g of dry substrate (gds). Among the evaluated strains, the Aspergillus niger mutant 11T53A14 was selected by presenting the best enzymatic production. For the fermentation tests, two substrates were also investigated: wheat bran and corn cob, both supplemented with olive oil. The best results were obtained with wheat bran. Additionally, three industrial by-products from corn oil refining (soapstock, stearin and fatty acids) were evaluated as substitutes to the olive oil in the function of lipases production inducer. Among them, soapstock and stearin were the best inducers, whereas fatty acids presented an inhibitor effect. The highest lipase activities using soapstock, stearin and fatty acids were 62.7 U/gds, 37.7 U/gds and 4.1 U/gds, respectively.

Fungal Lipase Production by Solid-State Fermentation

“…The rising interest in lipase production mainly lies on the wide industrial applications of the enzyme [4][5][6]. They recognize a wide variety of substrates and may catalyze many different reactions, such as hydrolysis or synthesis of esters bonds, alcoholysis, aminolysis, peroxidations, epoxidations and interesterifications.…”

“…Despite SmF is widely used in the enzyme industry and has advantages in process control and production yields, SSF represents an interesting alternative to produce industrial enzymes at lower costs [8,9]. SSF has many preferences to SmF for microbial enzyme production including: superior production yields and productivities, lower operating costs, less demands for asepsis control, cheaper fermentation media, higher oxygen distribution, fewer operational troubles, simpler equipments and control systems, lower energy consumption [6,10].…”

Lipase Production in Tray-Bioreactor via Solid State Fermentation under Desired Growth Conditions

“…3). Damaso and his coworkers [1] had demonstrated independent work carried out by Mahanta et al [26], the that olive oil concentration of 2% and 12% for wheat bran enrichment of substrate with maltose as carbon source and corn cob led to maximum lipase production from and NaNO as nitrogen source led to enhancement in Aspergillus niger. In another separate research done by Adinarayana et al…”

“…Lipases (glycerol ester hydrolases, EC 3.1.1.3) suitable sources for lipase production; since they are catalyze the hydrolysis of fats to produce fatty acids, more stable and can be obtained with much lower costs monoglycerides, diglycerides and glycerol [1,2]. compared to lipases produced from animals and plants [8].…”

“…microorganisms [8][9][10]. Microorganisms are known as residues which are classified in the latter group, provide alternative substrates for solid state fermentation; while the environmental pollution problems are resolved [1,11,12]. In past decades, a great number of studies Fungal Strain and Media Composition: The Rhizopus considering the production of lipases through submerged oryzae, PTCC 5176 employed in this research was fermentation have been conducted [13][14][15][16][17].…”