Olive pomace valorization by <i>Aspergillus</i> species: lipase production using solid‐state fermentation

Oliveira, Felisbela Maria Araújo; Moreira, Catarina; Salgado, José Manuel; Abrunhosa, Luı́s; Venâncio, Armando; Belo, Isabel

doi:10.1002/jsfa.7544

Cited by 42 publications

(35 citation statements)

References 39 publications

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“…In fact, the temperature in the tray‐type bioreactor over the fermentation time increased from 30 °C to 34.3 °C, after 1 day of fermentation. The increase of temperature above optimum level (30 °C) for lipase production by A. ibericus has a negative impact on final activity …”

Section: Resultsmentioning

confidence: 99%

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Lipase production by solid‐state fermentation of olive pomace in tray‐type and pressurized bioreactors

Oliveira

Salgado

Pérez-Rodríguez

et al. 2017

J of Chemical Tech & Biotech

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BACKGROUND Bioreactor type, sterilization and specific operational conditions are key factors for the scale‐up of solid‐state fermentation (SSF). This work deals with the lipase production by SSF of olive pomace (OP) in traditional tray‐type and pressurized bioreactors. Important aspects of SSF in bioreactors were studied, such as the need for sterilization and moisture content (MC) control. RESULTS At larger scale, there was no significant difference in lipase production between sterilized and unsterilized substrates, but MC control had significant impact. The production of lipase in a pressurized bioreactor, under air absolute pressure of 200 kPa and 400 kPa, was 2‐fold higher than in a tray‐type bioreactor using the same amount of substrate (500 g) and the same bed height. The protein content of substrate increased from 10 to 18% (w/w) after SSF and the fermented solid presented an antioxidant activity of 10 mmol Trolox kg‐1. CONCLUSIONS SSF in a pressurized bioreactor enabled efficient production of lipase with higher substrate bed height in contrast to that in a tray‐type bioreactor. The improvement in nutritional value of the substrate by SSF indicates its potential applicability in animal feed. © 2017 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 99%

“…The mixture of OP with WB resulted in optimum MC, of around 60% (w/w), without the need for its adjustment. Fermentations were carried out at 30 °C in an incubator for 7 days without agitation , . SSFs were performed using sterilized (121 °C, 200 kPa for 15 min) and unsterilized substrate.…”

Section: Methodsmentioning

confidence: 99%

Lipase production by solid‐state fermentation of olive pomace in tray‐type and pressurized bioreactors

Oliveira

Salgado

Pérez-Rodríguez

et al. 2017

J of Chemical Tech & Biotech

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“…The residual oil extraction recovered 75% of lipids from COP. The presence of lipids in solid substrate can induce the production of lipases, the production of lipases by A. ibericus using COP as solid substrate has already been studied by Oliveira et al (2016). The concentration of free protein, sugars and phenols was determined after water extraction.…”

Section: Effect Of Residual Oil Extraction On Physicochemical Charactmentioning

confidence: 99%

“…organic load, presence of antimicrobial compounds such phenols and lipids, low pH value and low nutrients concentration (Morillo et al, 2009). These barriers can be overcome in biotechnology processes by mixing with other wastes (Salgado et al, 2014a(Salgado et al, , 2015Oliveira et al, 2016) or by pre-treatments (Gianico et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Ultrasounds pretreatment of olive pomace to improve xylanase and cellulase production by solid-state fermentation

Leite

Salgado

Venâncio

et al. 2016

Bioresource Technology

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Olive mills generate a large amount of waste that can be revaluated. This work aim to improve the production lignocellulolytic enzymes by solid-state fermentation using ultrasounds pretreated olive mill wastes. The composition of olive mill wastes (crude and exhausted olive pomace) was compared and several physicochemical characteristics were significantly different. The use of both wastes in SSF was evaluated and a screening of fungi for xylanase and cellulase production was carried out. After screening, the use of exhausted olive pomace and Aspergillus niger led to the highest enzyme activities, so that they were used in the study of ultrasounds pre-treatment. The results showed that the sonication led to a 3-fold increase of xylanase activity and a decrease of cellulase activity. Moreover, the liquid fraction obtained from ultrasounds treatment was used to adjust the moisture of solid and a positive effect on xylanase (3.6-fold increase) and cellulase (1.2-fold increase) production was obtained.

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“…Many papers on lipase production using solid-state cultivation of fungi on different low-cost media derived from agroindustry residues have been published thus far, although no data of its successful commercialization on a large scale are available. With the purpose of lipase production, Aspergillus niger was cultivated on a variety of agricultural wastes [6][7][8][9], Rhizopus oryzae on sugarcane bagasse [10], Aspergillus species on olive pomace [11,12], Trichoderma strains on oil-palm fruit-bunch medium [13], Penicillium camemberti KCCM 11268 on wheat bran [14], Schizophyllum commune ISTL04 on Leucaena leucocephala seeds [15], etc.…”

Section: Introductionmentioning

confidence: 99%

Lipase Production by Solid-State Cultivation of Thermomyces Lanuginosus on By-Products from Cold-Pressing Oil Production

et al. 2019

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This study shows that by-products obtained after cold-pressing oil production (flex oil cake, hemp oil cake, hull-less pumpkin oil cake) could be used as substrates for the sustainable and cost-effective production of lipase when cultivating Thermomyces lanuginosus under solid-state conditions (T = 45 °C, t = 9 days). Lipase showed optimum activity at T = 40 °C. The produced lipase extract was purified 17.03-folds with a recovery of 1% after gel chromatography. Three different batch experiments were performed in order to test the possibility of using the lipase in biodiesel production. Experiments were performed with a commercial, unpurified enzyme, and partially purified lipase with sunflower oil and methanol as substrates in a batch reactor at 40 °C. During the experiments, the operational stability of the enzyme was studied. The obtained results clearly showed that produced crude and purified lipase can be used for biodiesel production, but the process needs some additional optimization. As for operation stability, it was noticed that the commercial enzyme was deactivated after 30 h, while produced crude enzyme remained 8.25% of its activity after 368 h.

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Olive pomace valorization by Aspergillus species: lipase production using solid‐state fermentation

Abstract: Aspergillus ibericus was found to be the most promising microorganism for lipase production using mixtures of OP and WB. © 2015 Society of Chemical Industry.

Cited by 42 publications

References 39 publications

Lipase production by solid‐state fermentation of olive pomace in tray‐type and pressurized bioreactors

Lipase production by solid‐state fermentation of olive pomace in tray‐type and pressurized bioreactors

Ultrasounds pretreatment of olive pomace to improve xylanase and cellulase production by solid-state fermentation

Lipase Production by Solid-State Cultivation of Thermomyces Lanuginosus on By-Products from Cold-Pressing Oil Production

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