Detection of Mycoplasma hyopneumoniae by polymerase chain reaction in swine presenting respiratory problems

Yamaguti, Maurício; Müller, E.; Piffer, A.I.; Kich, Jalusa Deon; Klein, Cátia Silene; Kuchiishi, Suzana Satomi

doi:10.1590/s1517-83822008000300011

Cited by 7 publications

(5 citation statements)

References 16 publications

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“…Frequently, concomitant occurrence of the two described patterns was observed. Furthermore, to prove the concomitant involvement of M. hyopneumoniae in lung lesions, 80 samples of pig lungs (10 samples from each visit) with final diagnosis of pneumonia were randomly chosen and submitted to PCR for amplification of the 16S rRNA m gene of M. hyopneumoniae (Yamaguti et al 2008), added to ROX as a reference dye. The extraction was performed with a commercial kit (MagMAX CORE Nucleic Acid Purifications, Thermo Fisher Scientific) according to the manufacturer's instructions, and the samples were stored at -20°C.…”

Section: Methodsmentioning

confidence: 99%

Causes of death in growing-finishing pigs in two technified farms in southern Brazil

et al. 2020

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The aim of this study was to investigate the main causes of death in growing-finishing pigs in southern Brazil. During a one-year period (from 2018 to 2019), two industrial pig herds (18 and 20 thousand pigs each farm) in southern Brazil were monitored along the four seasons of the year (12 days per season on each farm), in order to perform necropsies of all pigs that died in that period. The two farms had an average monthly mortality rate ranging from 0.94 to 3.93% in the evaluated months. At necropsy, tissues were collected, fixed in 10% formalin solution and processed routinely for histopathological examination. When necessary, samples were sent for bacterial culture and PCR to identify etiologic agents. A total of 601 necropsies were performed, with 94.9% of conclusive diagnoses. Infectious diseases corresponded to 64.4% of conclusive diagnosis and non-infectious diseases to 35.6%. The most prevalent causes of death were: pneumonia (33%), gastric ulcers (15.4%), circovirosis (9.9%), systemic bacterial embolism (5.4%), polyserositis (4.4%), dilated cardiomyopathy and torsion of abdominal organs (4.3% each), and bacterial pericarditis (3.4%). Regarding pneumonias (199/601), the main agents identified in these cases were Pasteurella multocida, Influenza A virus and Mycoplasma hyopneumoniae, mainly in associations.

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Section: Methodsmentioning

confidence: 99%

Causes of death in growing-finishing pigs in two technified farms in southern Brazil

et al. 2020

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“…A pool of these two swabs was cultured using routine methods for the isolation of bacterial pathogens of the respiratory tract of pigs (A. pleuropneumoniae, H. parasuis, B. bronchiseptica and P. multocida) (Quinn et al 2011). M. hyopneumoniae testing was undertaken through Nested PCR assay from individual tonsillar swabs, according to Yamaguti et al (2008).…”

Section: Methodsmentioning

confidence: 99%

Pasteurella multocida type A as the primary agent of pneumonia and septicaemia in pigs

et al. 2015

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In order to understand better the pathological aspects and spread of Pasteurella multocida type A as the primary cause of pneumonia in pigs, was made an experiment with intranasal inoculation of different concentrations of inocula [Group (G1): 108 Colony Forming Units (CFU)/ml; G2: 107 CFU/ml; G3: 106 CFU/ml and G4: 105 CFU/ml], using two pigs per group. The pigs were obtained from a high health status herd. Pigs were monitored clinically for 4 days and subsequently necropsied. All pigs had clinical signs and lesions associated with respiratory disease. Dyspnoea and hyperthermia were the main clinical signs observed. Suppurative cranioventral bronchopneumonia, in some cases associated with necrosuppurative pleuropneumonia, fibrinous pericarditis and pleuritic, were the most frequent types of lesion found. The disease evolved with septicaemia, characterized by septic infarctions in the liver and spleen, with the detection of P. multocida type A. In this study, P. multocida type A strain #11246 was the primary agent of fibrinous pleuritis and suppurative cranioventral bronchopneumonia, pericarditis and septicaemia in the pigs. All concentrations of inoculum used (105-108 CFU/ml) were able to produce clinical and pathological changes of pneumonia, pleuritis, pericarditis and septicemia in challenged animals.

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“…As amostras de DNA foram eluídas em 100 mL de água ultrapura e armazenadas a -20°C até a reação de polimerase em cadeia (PCR). Foram usadas PCR previamente descritas para amplificação de fragmento do gene da toxina Apx IV de A. pleuropneumoniae (Schaller et al 2001), para o gene 16S rRNA da P. multocida (Townsend et al 2001), para o gene 16S rRNA de H. parasuis (Oliveira et al 2001), para o gene 16S rRNA de M. hyopneumoniae (Yamaguti et al 2008) e para o gene 16S rRNA de M. hyorhinis (Stakenborg et al 2006). Foram consideradas positivas para os agentes as amostras que amplificaram fragmentos dos genes estudados.…”

Section: Methodsunclassified

Pericardite em suínos ao abate no Rio Grande Sul: avaliação de agentes bacterianos e lesões associadas

et al. 2014

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Pesq. Vet. Bras. 34 (7) The objective of the study was to identify the frequency of macroscopic and microscopic lesions and bacterial agents involved with pericarditis in slaughter pigs in the State of Rio Grande do Sul, Brazil. The samples were collected in slaughterhouses with Federal Inspection Service (SIF) between February and October, 2010. Condemnation due to pericarditis in the examined animals was 3.9% (299/7,571). Ninety one cases of pericarditis were examined and by histopathology 89% were chronic and 47% of the corresponding lungs showed chronic pleuritis, but there was no significant association between both lesions. The bacterial agents isolated from the hearts were Streptococcus spp., Pasteurella multocida, Haemophilus parasuis and Streptococcus suis. Bacterial DNA from Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae were the most frequently detected by PCR. There was significant association between isolation of P. multocida and Streptococcus spp. in the hearts and corresponding lungs. The results suggest that lung infection could act as a port of entry to the colonization of the adjacent pericardium. In spite of the fact that M. hyopneumoniae was the agent more frequently identified by PCR in the heart and corresponding lung, there was no significant association of the agent in the organs. This suggests that the infections were independent events. The other agents investigated did not show significant association between isolation or DNA detection in heart and corresponding lungs. Another important finding was the presence of coinfection between bacterial agents in 2% of the hearts and by PCR were identified bacterial DNA of two or more agents in 16.5% of the hearts. These results suggest that coinfections in cases of pericarditis need further investigation.

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Detection of Mycoplasma hyopneumoniae by polymerase chain reaction in swine presenting respiratory problems

Cited by 7 publications

References 16 publications

Causes of death in growing-finishing pigs in two technified farms in southern Brazil

Causes of death in growing-finishing pigs in two technified farms in southern Brazil

Pasteurella multocida type A as the primary agent of pneumonia and septicaemia in pigs

Pericardite em suínos ao abate no Rio Grande Sul: avaliação de agentes bacterianos e lesões associadas

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