Mycelial glucoamylases produced by the thermophilic fungus Scytalidium thermophilum strains 15.1 and 15.8: purification and biochemical characterization

Ferreira-Nozawa,; Rezende, J. L. M.; Guimarães, Luís Henrique Souza; Terenzi, Héctor Francisco; Jorge, J.A.; Polizeli, Maria de Lourdes Teixeira de Moraes

doi:10.1590/s1517-83822008000200027

Cited by 8 publications

(2 citation statements)

References 15 publications

(19 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A variety of downstream processing techniques such ion exchange chromatography, hydrophobic interaction chromatography, and gel filtration chromatography have been exploited for the purification of glucoamylase [1,[7][8][9][10][11]. But the flipside of these procedures is that they are expensive, time consuming, and are often multistep low-yield protocols, not suitable for large scale production.…”

Section: Introductionmentioning

confidence: 99%

Partitioning of thermostable glucoamylase in polyethyleneglycol/salt aqueous two-phase system

Vinayagam

Murty

2015

Bioresour. Bioprocess.

View full text Add to dashboard Cite

Background: A major challenge in downstream processing is the separation and purification of a target biomolecule from the fermentation broth which is a cocktail of various biomolecules as impurities. Aqueous two phase system (ATPS) can address this issue to a great extent so that the separation and partial purification of a target biomolecule can be integrated into a single step. In the food industry, starch production is carried out using thermostable glucoamylase. Humicola grisea serves as an attractive source for extracellular production of glucoamylase. Results: In the present investigation, the possibility of using polyethylene glycol (PEG)/salt-based ATPS for the partitioning of glucoamylase from H. grisea was investigated for the first time. Experiments were conducted based on one variable at a time approach in which independent parameters like PEG molecular weight, type of phase-forming salt, tie line length, phase volume ratio, and neutral salt concentration were optimized. It has been found that the PEG 4000/potassium phosphate system was suitable for the extraction of glucoamylase from the fermentation broth. From the results, it was observed that, at a phase composition of 22 % w/w PEG 4000 and 12 % w/w phosphate in the presence of 2 % w/w NaCl and at pH 8, glucoamylase was partitioned into the salt-rich phase with a maximum yield of 85.81 %.

show abstract

Section: Introductionmentioning

confidence: 99%

Partitioning of thermostable glucoamylase in polyethyleneglycol/salt aqueous two-phase system

Vinayagam

Murty

2015

Bioresour. Bioprocess.

View full text Add to dashboard Cite

show abstract

“…Segundo TANGPHATSORNRUANG et al, 2005;CARVALHO et al, 2008;FERREIRA-NOZAWA et al, 2008;PEIXOTO-NOGUEIRA et al, 2008;RAJAGOPALAN;KRISHNAN, 2008;SOUZA, MAGALHÃES, 2010;HASHEMI;DARWISH, 2010).…”

Section: Enzimas Amilolíticasmentioning

confidence: 99%

Avaliação do potencial de produção de enzimas amilolíticas por fungos isolados da compostagem – uma enzima de interesse biotecnológico.

Nunes¹,

Nunes²

View full text Add to dashboard Cite

A compostagem é um processo de decomposição da matéria orgânica e que ocorre devido à produção de enzimas extracelulares provenientes de microrganismos presentes no sistema. Dentre as enzimas produzidas destaca-se as de atividade amilolítica. Essas enzimas são amplamente aplicadas em processos biotecnológicos nas indústrias têxteis, papel e celulose, couro, detergentes, bebidas destiladas, cervejas, panificação, cereais para alimentação infantil, produção de xaropes, ração animal, indústria química e farmacêutica. O presente trabalho teve como objetivo o isolamento de fungos com capacidade de produzir enzima amilolíticas, além de acompanhar a variação de temperatura em um processo de compostagem. As coletas foram realizadas a partir do 10° dia após a montagem da composteira e repetidas a cada 15 dias, totalizando sete coletas. No momento da coleta foi feita a aferição da temperatura e foram retiradas amostras para isolamento de fungos. A produção das enzimas foi verificada por meio do método de difusão em gel de ágar, em placas de Petri contendo amido, para a determinação da atividade amilolítica. Foram calculados os índices enzimáticos (IE) por meio da relação entre o halo de degradação do substrato e o halo de crescimento fúngico. A temperatura no tanque de compostagem variou entre 36°C e 43°C. No decorrer das coletas foram obtidos 36 isolados fúngicos, sendo que seis foram obtidos na fase termofílica. Os maiores IE foram obtidos entre os fungos isolados nas coletas 3, 6 e 7. Diante dos altos valores de IE obtidos pode-se afirmar que o tanque de compostagem representa um local potencial para o isolamento de microrganismos com capacidade de produzir enzimas de interesse industrial, com o consequente desenvolvimento de tecnologias enzimáticas.

show abstract

Purification and biochemical characterization of extracellular glucoamylase from Paenibacillus amylolyticus strain

Lincoln

More²,

2019

J Basic Microbiol

View full text Add to dashboard Cite

In the present study, glucoamylase produced from a soil bacterium Paenibacillus amylolyticus NEO03 was cultured under submerged fermentation conditions. The extracellular enzyme was purified by starch adsorption chromatography and further by gel filtration, with 2.73‐fold and recovery of 40.02%. The protein exhibited molecular mass of ∼66,000 Da as estimated by SDS–PAGE and depicted to be a monomer. The enzyme demonstrated optimum activity at pH range 6.0–7.0 and temperature range 30–40 °C. Glucoamylase was mostly activated by Mn2+ metal ions and depicted no dependency on Ca2+ ions. The enzyme preferentially hydrolyzed all the starch substrates. High substrate specificity was demonstrated towards soluble starch and kinetic values Km and Vmax were 2.84 mg/ml and 239.2 U/ml, respectively. The products of hydrolysis of soluble starch were detected by thin layer chromatography which showed only D‐glucose, indicating a true glucoamylase. The secreted glucoamylase from P. amylolyticus strain possesses properties suitable for saccharification processes such as biofuel production.

show abstract

Mycelial glucoamylases produced by the thermophilic fungus Scytalidium thermophilum strains 15.1 and 15.8: purification and biochemical characterization

Cited by 8 publications

References 15 publications

Partitioning of thermostable glucoamylase in polyethyleneglycol/salt aqueous two-phase system

Partitioning of thermostable glucoamylase in polyethyleneglycol/salt aqueous two-phase system

Avaliação do potencial de produção de enzimas amilolíticas por fungos isolados da compostagem – uma enzima de interesse biotecnológico.

Purification and biochemical characterization of extracellular glucoamylase from Paenibacillus amylolyticus strain

Contact Info

Product

Resources

About