Partitioning of thermostable glucoamylase in polyethyleneglycol/salt aqueous two-phase system

Vinayagam, Ramesh; Murty, V. Ramachandra

doi:10.1186/s40643-015-0056-6

Cited by 14 publications

(6 citation statements)

References 36 publications

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“…Similar interrelationship was also found by [41] by using PEG-phosphate systems with PEG molecular weight 600 to 20,000. Similar results were observed by [42][43][44][45].…”

Section: Effect Of Peg Molecular Weight On Partitioning Of Proteasesupporting

confidence: 89%

Utilization of Integrative Technique for Partial Recovery of Proteases from Soil Microbes

Nawab

Aasim

Saddique

et al. 2018

Period. Polytech. Chem. Eng.

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Aqueous two-phase system (ATPS) is an efficient, cost effective, fast, simple and ecofriendly method for the recovery of biomolecules. In the present study, an ATPS composed of polyethylene glycol and ammonium sulphate (NH4)2SO4 was used for the partial purification of proteases from microbial source. The effects of different parameters such as molecular weight of PEG (4000, 6000 and 10000), concentration of PEG (15, 17.5 and 20 %) and concentration of (NH4)2SO4 (7.5, 8.3, 9.1 and 9.9 %) on the partitioning behavior of proteases at room temperature were investigated. Generally, increasing the concentration of PEG and (NH4)2SO4 moved the protease to the top i.e., polymer-rich phase. Increasing the molecular weight of PEG from 4000 to 10000 the partition coefficient decreased subsequently. The highest partition coefficient i.e., 3.32 and maximum activity i.e., 16.06 soxhlet unit was found in an optimum system composed of 20 % PEG 4000 and 9.9 % (NH4)2SO4.

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“…Similar interrelationship was also found by [41] by using PEG-phosphate systems with PEG molecular weight 600 to 20,000. Similar results were observed by [42][43][44][45].…”

Section: Effect Of Peg Molecular Weight On Partitioning Of Proteasesupporting

confidence: 89%

Utilization of Integrative Technique for Partial Recovery of Proteases from Soil Microbes

Nawab

Aasim

Saddique

et al. 2018

Period. Polytech. Chem. Eng.

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“…In Figure 9 the addition of NaCl at a concentration range (0.1-0.5 M) to the PEG/SPH system shows an increment in the recovery percent and partition coefficient, higher values recorded at (NaCl 0.5 M) were 98.97% of recovery, and 105.34 for partition coefficient. [20,21,23,25,31,33,42,51] all went with similar findings and there is no any incompatible opinion. However, a different behavior was recorded in the PEG/Dextran system, as shown in Figure 10.…”

Section: Effect Of Nacl Addingsupporting

confidence: 67%

Application of Aqueous Two-Phase Systems in the Extraction of Bovine Serum Albumin

Mahdi¹,

Hameed²,

Ali³

2023

JOPC

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Protein partitioning in an Aqueous Two-Phase System (ATPS) has been found to be a powerful method for extracting and separating mixtures of biomolecules. In Aqueous Two-Phase Systems, many factors influence the Partition coefficient (K) (which is the ratio of protein concentration in the top phase to that in the bottom phase) and the Recovery percent (Rec%). Two systems of ATPS were used: first, polyethylene glycol (PEG4000)/sodium phosphate (SPH), and second, PEG4000/Dextran. The behavior of Rec% and (K) of pure Bovine Serum Albumin (BSA) in (ATPS) has been investigated throughout the study of the effects of five parameters: (temperature (T), the concentration of polyethylene glycol (PEG4000), the concentration of sodium phosphate or Dextran, pH, and the addition of sodium chloride as a supporting agent). In both systems, pH was the more significant parameter on a Recovery percent (Rec%) and partition coefficient (K). In the first system, the maximum (Rec%) and (K) was 98.08% and 50.99, respectively, at a temperature of 31°C, the concentration of PEG4000 of 1.5 g/10 ml, the concentration of sodium phosphate at 2.4 g/10 ml, pH 10, and the concentration of NaCl at 0.5 M. While in the second system, the parameter that has a more significant effect on (Rec%) and (K) was the temperature. The maximum (Rec%) and (K) were 97.54% and 39.7 respectively at a temperature of 31°C, with a concentration of PEG4000 1.5 g/10 ml, the concentration of Dextran 2.4 g/10 ml, pH 5, and concentration of NaCl at 0.1 M.

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“…Therefore, the upper phase is rich in PEG, and the lower phase is rich in inorganic salt 29 . Because of its lower cost, lower viscosity, and high selectivity, the polymer/salt-based two-phase aqueous system is more widely employed 30 – 32 .…”

Section: Introductionmentioning

confidence: 99%

An efficiency strategy for cobalt recovery from simulated wastewater by biphasic system with polyethylene glycol and ammonium sulfate

Amjad

Asadollahzadeh

Torkaman

et al. 2022

Sci Rep

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Today, biphasic aqueous systems have received more attention than conventional separation methods due to their advantages, such as biocompatibility, low cost, and easy operation. The extraction of cobalt ions from the aqueous phase with the absence and the presence of other ions was investigated using polyethylene glycol, and ammonium sulfate salt without using an extractant. The efficiency was evaluated using operating parameters such as aqueous pH, salt and polymer concentrations, phase volume ratio, and initial metal concentration. The higher temperature, and the lower aqueous pH showed a maximum transfer rate for cobalt ions into the PEG1000 phase. Extraction efficiency under optimal conditions equal to 50% (w/w) polyethylene glycol 1000, 4 M ammonium sulfate, aqueous pH = 2, and 15 min extraction time was over 98%. Results from infrared spectroscopy, and thermo-gravimetric analysis illustrated the presence of the PEG-cobalt ion complex. The observation demonstrated that the biphasic system is the proper technology for wastewater purification.

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Partitioning of thermostable glucoamylase in polyethyleneglycol/salt aqueous two-phase system

Cited by 14 publications

References 36 publications

Utilization of Integrative Technique for Partial Recovery of Proteases from Soil Microbes

Utilization of Integrative Technique for Partial Recovery of Proteases from Soil Microbes

Application of Aqueous Two-Phase Systems in the Extraction of Bovine Serum Albumin

An efficiency strategy for cobalt recovery from simulated wastewater by biphasic system with polyethylene glycol and ammonium sulfate

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