Bovine papillomavirus type 4 L1 gene transfection in a Drosophila S2 cell expression system: absence of L1 protein expression

Góes, Luiz Gustavo Bentim; Freitas, Antonio Carlos de; Ferraz, O. P.; Rieger, Tania T.; Santos, Juliana Ferreira dos; Pereira, Alexandre da Costa; Beçak, Willy; Lindsey, Charles Julian; Stocco, Rita de Cássia

doi:10.1590/s1517-83822008000100001

Cited by 11 publications

(3 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to these latter authors, Debruyne et al (2005), there are 14 validly described Campylobacter species. More recently, Fernández et al (2008) stated that the genus comprises 20 species and subspecies. However, other authors have stated that there are 16 species with a further six subspecies within the genus Campylobacter (On, 2001; Foster et al, 2004).…”

Section: The Genus Campylobactermentioning

confidence: 99%

Campylobacter spp. as a Foodborne Pathogen: A Review

et al. 2011

View full text Add to dashboard Cite

Campylobacter is well recognized as the leading cause of bacterial foodborne diarrheal disease worldwide. Symptoms can range from mild to serious infections of the children and the elderly and permanent neurological symptoms. The organism is a cytochrome oxidase positive, microaerophilic, curved Gram-negative rod exhibiting corkscrew motility and is carried in the intestine of many wild and domestic animals, particularly avian species including poultry. Intestinal colonization results in healthy animals as carriers. In contrast with the most recent published reviews that cover specific aspects of Campylobacter/campylobacteriosis, this broad review aims at elucidating and discussing the (i) genus Campylobacter, growth and survival characteristics; (ii) detection, isolation and confirmation of Campylobacter; (iii) campylobacteriosis and presence of virulence factors; and (iv) colonization of poultry and control strategies.

show abstract

Section: The Genus Campylobactermentioning

confidence: 99%

Campylobacter spp. as a Foodborne Pathogen: A Review

et al. 2011

View full text Add to dashboard Cite

show abstract

“…The idea of developing a vaccine to combat BPV began with the infection of Shope papillomas extract in the 1940 decade ( Shope, 1937 ). Since then, different vaccine models were proposed ( Jarret et al , 1991 ; Gaukroger et al , 1996 ; Góes et al , 2008 ; Love et al , 2012 ; Mazzuchelli-de-Souza et al , 2013 ). However, none of them became a commercial product.…”

Section: Prophylactic and Therapeutic Vaccines Against Bpv And Hpvmentioning

confidence: 99%

Papillomaviruses: a systematic review

et al. 2017

Self Cite

View full text Add to dashboard Cite

In the last decades, a group of viruses has received great attention due to its relationship with cancer development and its wide distribution throughout the vertebrates: the papillomaviruses. In this article, we aim to review some of the most relevant reports concerning the use of bovines as an experimental model for studies related to papillomaviruses. Moreover, the obtained data contributes to the development of strategies against the clinical consequences of bovine papillomaviruses (BPV) that have led to drastic hazards to the herds. To overcome the problem, the vaccines that we have been developing involve recombinant DNA technology, aiming at prophylactic and therapeutic procedures. It is important to point out that these strategies can be used as models for innovative procedures against HPV, as this virus is the main causal agent of cervical cancer, the second most fatal cancer in women.

show abstract

“…The BPV icosahedral capsid has 360 copies of the L1 protein of 55 kDa, organized in 72 capsomers and approximately 12 copies of the L2 protein with 39 kDa (Campo, 1997;Góes et al, 2008;Meinke and Meinke, 1981) having a molecular weight of 5.2 × 10 6 Da (Orth et al, 1977). The BPV genome is divided into three regions: early, late and long control, separated by two polyadenylation sites http://dx.doi.org/10.1016/j.jviromet.2014.07.029 0166-0934/© 2014 Elsevier B.V. All rights reserved.…”

mentioning

confidence: 99%

Bovine papillomavirus isolation by ultracentrifugation

Giovanni

Melo

Diniz

et al. 2014

Journal of Virological Methods

View full text Add to dashboard Cite

The bovine papillomavirus (BPV) is the etiological agent of bovine papillomatosis, which causes significant economic losses to livestock, characterized by the presence of papillomas that regress spontaneously or persist and progress to malignancy. Currently, there are 13 types of BPVs described in the literature as well as 32 putative new types. This study aimed to isolate viral particles of BPV from skin papillomas, using a novel viral isolation method. The virus types were previously identified with new primers designed. 77 cutaneous papilloma samples of 27 animals, Simmental breed, were surgically removed. The DNA was extracted and subjected to PCR using Delta-Epsilon and Xi primers. The bands were purified and sequenced. The sequences were analyzed using software and compared to the GenBank database, by BLAST tool. The viral typing showed a prevalence of BPV-2 in 81.81% of samples. It was also detected the presence of the putative new virus type BR/UEL2 in one sample. Virus isolation was performed by ultracentrifugation in a single density of cesium chloride. The method of virus isolation is less laborious than those previously described, allowing the isolation of complete virus particles of BPV-2.

show abstract

Bovine papillomavirus type 4 L1 gene transfection in a Drosophila S2 cell expression system: absence of L1 protein expression

Cited by 11 publications

References 8 publications

Campylobacter spp. as a Foodborne Pathogen: A Review

Campylobacter spp. as a Foodborne Pathogen: A Review

Papillomaviruses: a systematic review

Bovine papillomavirus isolation by ultracentrifugation

Contact Info

Product

Resources

About