Distribution of virulence genes sefC, pefA and spvC in Salmonella Enteritidis phage type 4 strains isolated in Brazil

Castilla, Karina Salvagni; Ferreira, Claudete Serrano Astolfi; Moreno, Andréa Micke; Nunes, Iolanda Aparecida; Ferreira, Antônio José Piantino

doi:10.1590/s1517-83822006000200007

Cited by 23 publications

(16 citation statements)

References 29 publications

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“…Our results for the virulence plasmid gene spvC were similar to those found by other authors (Oliveira et al 2003, Castilla et al 2006, Amini et al 2010). There are also other studies that have found lower frequencies for this gene in strains of avian origin (Okamoto et al 2009, Derakhshandeh et al 2013, Moussa et al 2013.…”

Section: Discussionsupporting

confidence: 92%

Detection of virulence-associated genes in Salmonella Enteritidis isolates from chicken in South of Brazil

et al. 2013

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RESUMO.-[Detecção de genes associados à virulência em cepas de Salmonella Enteritidis isoladas de frangos na região sul do Brasil.] Salmonella spp. estão entre os principais agentes causadores de doenças transmitidas por alimentos, e o sorovar Salmonella Enteritidis é o mais frequentemente isolado no mundo. A virulência de Salmonella spp. e a sua interação com o hospedeiro são processos complexos que envolvem fatores de virulência para sobreviver às defesas do hospedeiro. O objetivo deste estudo foi detectar genes de virulência em cepas de S. Enteritidis isoladas a partir de fontes avícolas no sul do Brasil. Ensaios de PCR foram desenvolvidos para a detecção de nove genes (lpfA, agfA, sefA, invA, hilA, avrA, sopE, sivH are considered the main agents of foodborne disease and Salmonella Enteritidis is one of the most frequently isolated serovars worldwide. The virulence of Salmonella spp. and their interaction with the host are complex processes involving virulence factors to overcome host defenses. The purpose of this study was to detect virulence genes in S. Enteritidis isolates from poultry in the South of Brazil. PCR-based assays were developed in order to detect nine genes (lpfA, agfA, sefA, invA, hilA, avrA, sopE, sivH and spvC) associated with the virulence in eighty-four isolates of S. Enteritidis isolated from poultry. The invA, hilA, sivH, sefA and avrA genes were present in 100% of the isolates; lpfA and sopE were present in 99%; agfA was present in 96%; and the spvC gene was present in 92%. It was possible to characterize the isolates with four different genetic profiles (P1, P2, P3 and P4), as it follows: P1, positive for all genes; P2, negative only for spvC; P3, negative for agfA; and P4, negative for lpfA, spvC and sopE. The most prevalent profile was P1, which was present in 88% of the isolates. Although all isolates belong to the same serovar, it was possible to observe variations in the presence of these virulence-associated genes between different isolates. The characterization of the mechanisms of virulence circulating in the population of Salmonella Enteritidis is important for a better understanding of its biology and pathogenicity. The frequency of these genes and the establishment of genetic profiles can be used to determine patterns of virulence. These patterns, associated with in vivo studies, may help develop tools to predict the ability of virulence of different strains.

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Section: Discussionsupporting

confidence: 92%

Detection of virulence-associated genes in Salmonella Enteritidis isolates from chicken in South of Brazil

et al. 2013

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“…They reported that the prevalence of these genes in poultry and bovine S. enteritidis isolates were 88.6 and 100 % respectively, while in the present study the presence of spvB and spvC genes in poultry isolated S. enteritidis was 46.6 % (7/15) and 93.3 % (14/15) respectively. Another study has reported the lack of spvC gene in 7.2 % (8/110) of the S. enteritidis samples from human, pig, and poultry sources [28]. Zahraei et al [27] showed that the spv genes were not detected in 5.9 % of isolates of the S. enteritidis as compared with 7.4 % of the isolates reported by Pan and Liu [29].…”

Section: Discussionmentioning

confidence: 98%

Association of Three Plasmid-Encoded spv Genes Among Different Salmonella Serotypes Isolated from Different Origins

2012

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The virulence plasmid associated Salmonella plasmid virulence (spv) locus is strongly concomitant with strains that cause non typhoid bacteremia. The spv region contains three genes required for the virulence, the positive transcriptional regulator spvR and two structural genes spvB and spvC. The purpose of this study was to investigate the presence of these three genes among salmonella serotypes isolated from different sources. A collection of 60 salmonella serotypes from different sources were used. Polymerase chain reaction was carried out for the presence of these genes using specific primers. The prevalence of spvB, spvC, and spvR genes were 26 (43.3 %), 44 (73.3 %), and 28 (46.6 %), respectively. The findings revealed that the distribution of these genes was dissimilar among these serotypes. Many of the human pathogenic salmonella strains which can be transmitted by animals may have these genes and can be very injurious for public health.

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“…In a Brazilian study, Salmonella isolates were classified into four genetic profiles based on the distribution of spvC, sefC, and pefA genes [45].…”

Section: Discussionmentioning

confidence: 99%

Virulence genotypes of clinical SalmonellaSerovars from broilers in Egypt

Ammar

Mohamed

El-Hamid

et al. 2016

J Infect Dev Ctries

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Introduction: Salmonella serovars are one of the primary foodborne pathogens. Poultry consumption is responsible for the majority of disease cases worldwide. The prevalence of virulence determinants among Salmonella serovars appears to be lacking in Egypt. Therefore, this study investigated the occurrence, antibiotic resistance patterns, and virulence gene profiling of Salmonella serovars in broilers. Methodology: Three hundred samples from broiler chickens were examined for the presence of Salmonella by standard microbiological techniques. All Salmonella isolates were tested for their sensitivity against ten antibiotics and subjected to virulence genotyping by polymerase chain reaction (PCR). Results: The overall isolation percentage of Salmonella was 17%. Seven different serovars were found, with the main one being Salmonella Typhimurium (52.94%). Salmonella isolates were sensitive to most of the tested antibiotics, but they exhibited absolute resistance against amoxicillin/clavulanic acid. Nine Salmonella strains (52.94%) were resistant to at least three antibiotics. Further PCR investigations into 17 Salmonella strains revealed different distribution patterns of eight virulence determinants among the isolates. The invA gene was the most prevalent one (100%), followed by hilA (88.24%), stn (58.82%), and fliC genes (52.94%), while each of sopB and pefA genes had a similar prevalence (41.18%), and sefC and spvC genes had the lowest prevalence (11.76 and 5.88%, respectively). PCR genotyping allowed grouping of Salmonella strains into ten genetic profiles. Conclusions: These results will help in understanding the spread of virulence genotypes and antibiotic resistance among Salmonella serovars in broilers.

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Distribution of virulence genes sefC, pefA and spvC in Salmonella Enteritidis phage type 4 strains isolated in Brazil

Cited by 23 publications

References 29 publications

Detection of virulence-associated genes in Salmonella Enteritidis isolates from chicken in South of Brazil

Detection of virulence-associated genes in Salmonella Enteritidis isolates from chicken in South of Brazil

Association of Three Plasmid-Encoded spv Genes Among Different Salmonella Serotypes Isolated from Different Origins

Virulence genotypes of clinical SalmonellaSerovars from broilers in Egypt

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