Clonal characterization of Streptococcus mutans strains by multilocus enzyme electrophoresis

Rosa, Rosimeire Takaki; Napimoga, Marcelo Henrique; Höfling, José Francisco; Gonçalves, Reginaldo Bruno; Rosa, Edvaldo Antônio Ribeiro

doi:10.1590/s1517-83822006000100003

Cited by 3 publications

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References 13 publications

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“…The results provided some scientific validation for the traditional use of M. indica leaf extract which inhibited some characteristics of S. mutans and thus may be considered beneficial for oral care. [ 37 ] Therefore, this study provides a significant contributor to antibacterial and antioxidant activities. Of interest would be M. indica , which had the best antibacterial activity as well as generally the best antioxidant activity.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial activity of Azadirachta indica, Pongamia pinnata, Psidium guajava, and Mangifera indica and their mechanism of action against Streptococcus mutans

Bodiba¹,

Prasad

Srivastava

et al. 2018

Phcog Mag

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Background:Curative plants have reportedly been used to make chewing sticks/toothbrushes intended for the treatment of oral diseases.Objective:The in vitro antibacterial activities of Azadirachta indica, Pongamia pinnata, Psidium guajava, and Mangifera indica were evaluated against Streptococcus mutans, along with the cytotoxicity and antioxidant and synergistic potentials. The effect of M. indica on the expression of crucial virulence genes spaP and gtfB of S. mutans was determined.Materials and Methods:The antibacterial activity was determined using a modified microdilution method. The antioxidant potential was evaluated using diphenyl picrylhydrazyl (DPPH), Griess reagent, and nitroblue tetrazolium calorimetric assays. The synergistic activity was investigated using a modified checkerboard method, while the cytotoxicity was determined according to a cell proliferation 2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide salt assay. Reverse transcription was the chosen method for determining the difference in expression of the spaP and gtfB genes after treatment with the plant sample.Results:M. indica and A. indica had the highest antibacterial activity at concentrations of 0.3 mg/ml and 6.25 mg/ml, respectively. A. indica had the best free radical scavenging of DPPH, exhibiting 50% inhibition at 28.72 μg/ml; while M. indica showed better superoxide scavenging potential than the positive control quercetin. Both M. indica and A. indica had adequate activity against the nitric oxide-free radical (12.87 and 18.89 μg/ml, respectively). M. indica selectively reduced the expression of the gtfB gene, indicating a mechanism involving Glucotranferases, specifically targeting bacterial attachment.SUMMARY Mangifera indica and Azadirachta indica had very good antibacterial activity against Streptococcus mutans and moderate toxicity against Vero cellsM. indica had the best antioxidant capacity overallM. indica reduced the expression of gtfB gene at 0.5 mg/ml. Abbreviations used: AA: Ascorbic acid; BHI: Brain–heart infusion; CHX: Chlorhexidine; DPPH: Diphenyl picrylhydrazyl; DMSO: Dimethlysulfoxide; NBT: Nitroblue tetrazolium; NO: Nitric oxide;

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Section: Discussionmentioning

confidence: 99%

Antibacterial activity of Azadirachta indica, Pongamia pinnata, Psidium guajava, and Mangifera indica and their mechanism of action against Streptococcus mutans

Bodiba¹,

Prasad

Srivastava

et al. 2018

Phcog Mag

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Typing of Streptococcus mutans strains isolated from caries free and susceptible subjects by multilocus enzyme electrophoresis

Tahmourespour¹,

Nabinejad²,

Shirian³

et al. 2013

Braz. J. Microbiol.

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Isoenzymatic genotyping of Staphylococcus aureus from dairy cattle and human clinical environments reveal evolutionary divergences

Boriollo

Bassi

Höfling

2018

Rev. Inst. Med. trop. S. Paulo

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Background The genetic variability of 610 S. aureus isolates from the hands of professional dentists (A), dental clinic environment air (B), bovine milk from cows with and without mastitis (C), an insufflator for milking equipment (D) and milking environment air (E) was studied by isoenzyme genotyping and genetic and cluster analysis.Results Monoclonal and polyclonal patterns of S. aureus were detected in every bacterial population; however, isolates belonging to the same strain were not found among the populations, suggesting the genetic heterogeneity and the intrapopulation spread of strains. Genetic relationship analysis revealed the co-existence of highly related strains at low frequency among populations.Conclusion The data suggest that some strains can adapt and colonize new epidemiologically unrelated habitats. Consequently, the occurrence of an epidemiological genotypic identity can assume a dynamic character (spread to new habitats), however infrequently. A tendency of microevolutionary and genetic divergences among populations of S. aureus from human sources (AB) and bovine milk (DE), and especially the mammary quarter (C), is also suggested. This research can contribute to the knowledge on the distribution and dissemination of strains and the implementation of control measures and eradication of S. aureus in important dental clinic environments, as well as animal environments and dairy production.

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Clonal characterization of Streptococcus mutans strains by multilocus enzyme electrophoresis

Cited by 3 publications

References 13 publications

Antibacterial activity of Azadirachta indica, Pongamia pinnata, Psidium guajava, and Mangifera indica and their mechanism of action against Streptococcus mutans

Antibacterial activity of Azadirachta indica, Pongamia pinnata, Psidium guajava, and Mangifera indica and their mechanism of action against Streptococcus mutans

Typing of Streptococcus mutans strains isolated from caries free and susceptible subjects by multilocus enzyme electrophoresis

Isoenzymatic genotyping of Staphylococcus aureus from dairy cattle and human clinical environments reveal evolutionary divergences

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