Model of experimental infection in healthy and immunosuppressed swiss albino mice (Mus musculus) using Candida albicans strains with different patterns of enzymatic activity

Abstract

With the purpose to evaluate Candida albicans virulence in vivo, two strains selected were based on their phospholipase and proteinase activity, and used in a model of experimental infection. One strain, isolated from vaginal secretion, was stocked at the URM Culture Collection for 43 years and presented high phospholipase (Pz=0.217) and proteinase (1.386 U.mL -1 ) activity. The other strain was a fresh strain isolated from oropharyngeal secretion of an AIDS patient, and presented low phospholipase (Pz=0.482) and proteinase (0.780 U.mL -1 ) activity. The strains were inoculated via intraperitoneum in immunosuppressed and non-immunosuppressed mice (Mus musculus) and the infection was evaluated over a period of 21 days. Liver, spleen, lungs and kidneys were aseptically removed and the blood of the animals was collected every 72 h. The number of colony forming units (c.f.u) isolated from each organ was counted and a histopathologic examination was performed. The freshly isolated strain was more virulent than the stocked strain, as shown by the number of positive cultures and severity of the lesions observed at the histopathologic examination. A correlation between the in vitro enzymatic activity and the in vivo virulence was not observed.

SystemicCandida parapsilosisInfection Model in Immunosuppressed ICR Mice and Assessing the Antifungal Efficiency of Fluconazole

“…Many regents have been used to obtain the immunosuppressed mice, for example, cortisone acetate and hydrocortisone succinate [ 11 ]. Here, we used CY to induce immunosuppression in ICR mice.…”

Dataset on antitumor properties of silver nanoparticles from Gloriosa superba (L.) seed on Dalton Lymphoma Ascites (DLA) tumor: Facile and biocompatible approach

“…in vivo cytotoxic studies were carried out using the ED 50 of methanol extract of G. superba seed and AgNPs to follow the antitumor activity in terms of increase in life Span and histological examination of seven groups (Groups A to G) of (6 mice/group) Swiss albino mice transplanted with DLA tumor cells [8] .…”