Utilização de frutooligossacarídeo por estreptococos mutans in vitro

Abstract

Neosugar is the trade name of a fructooligosaccharide (FOS) whose utilization by oral bacteria is not well known yet. The aim of the present study was to evaluate in vitro the effect of this product on the growth, fermentation and production of plaque by mutans streptococci: S. mutans, serotypes c, e and f, S. sobrinus, serotype d, S. downei, serotype h, S. cricetus, serotype a and S. rattus, serotype b. The evaluation of growth was carried out in Brain Heart Infusion (BHI) broths containing or not sucrose and FOS and in buffered broths having glucose or FOS as carbon sources, through optical density reading in spectrophotometer after 24 hours of incubation at 37 degrees C. Thereafter the reading of pH was made in the same media. The plaque produced on glass sticks in BHI broths containing 5% sucrose or FOS was weighed and carbohydrates and proteins were assayed. The possible cariogenicity of Neosugar was confirmed, since it sustained the same growth and intensity of fermentation of sucrose in BHI broth for all streptococci and permitted in vitro production of plaque by some of them. The amount of plaque as well as its content of proteins and carbohydrates were smaller than those produced with sucrose, although the difference was statistically significant only for carbohydrates.

Biofilm Formation on Different Materials Used in Oral Rehabilitation

“…Saliva was stimulated by neutral chewing gum previously immersed in deionized water for 24 h. During the first minute, the saliva was swallowed, then 3 mL was taken from each individual and mixed under vortex. After mixing, the saliva was diluted (1:5) in a culture medium (BHI, Sigma-Aldrich, St. Louis, MO, USA) containing 5% sucrose (BHI 5% S) (16). For this dilution, 6 mL were collected from saliva and mixed in 24 mL of BHI 5% S. The pH of the culture medium with saliva was recorded at the first moment and after 24 or 48 h of biofilm growth using a digital potentiometer (Inolab1 and…”

“…However, few studies have reported the adhesion of oral biofilms on zirconia-based structures. There are different in vitro and in vivo methodologies to evaluate the biofilm density, composition and morphology, using human or modified artificial saliva (13)(14)(15)(16). A combination of different experimental approaches including microbiological and microscopic techniques can be useful to assess the in vitro biofilm growth on novel materials or structures applied in oral rehabilitation.…”