Ação da pancreatina na obtenção de hidrolisados protéicos de soro de leite com elevado teor de oligopeptídeos

Preparation and analysis of hydrolysates from whey protein concentrate using the proteases from Bacillus licheniformis and Aspergillus oryzae

“…1, the hydrolysates were resolved in four fractions: F1, from 13.0 to 17.0 min (large peptides, with more than seven amino acids residues), F2, from 17.0 to 20.5 min (medium peptides, with 4-7 amino acids residues), F3, from 20.5 to 21.5 min (di-and tripeptides) and F4, from 21.5 to 32.0 min (free amino acids). This result confirms those of previous studies from our group that used the same technique for fractionating enzymatic hydrolysates obtained from casein (Morais et al, 2005), rice (Bizzotto et al, 2006a,b), skim milk (Lopes et al, 2005), whey (Delvivo et al, 2006;Silva et al, 2007;Biasutti et al, 2008;Souza et al, 2008), whey protein concentrate (Afonso et al, , 2009Silva et al, 2010) and wheat flour (Bello de Arau´jo, 2009). Shown in Fig.…”

Perfil Peptídico De Hidrolisados Proteicos Da Farinha De Trigo

“…A técnica de SE-HPLC, utilizada no presente trabalho, permitiu a caracterização dos hidrolisados proteicos, especialmente com relação ao fracionamento de peptídeos com massas moleculares inferiores a 1.000 Da. Este resultado está de acordo com o apresentado por outros trabalhos que empregaram a mesma técnica, visando ao fracionamento de hidrolisados enzimáticos obtidos de diversas fontes proteicas, e em condições de hidrólise variadas, dentre os quais destacam-se os realizados com caseína (Morato et al 2000, Carreira et al 2004, Morais et al 2005, arroz (Lopes et al 2008), leite desnatado (Lopes et al 2005, Soares et al 2006e 2007, soro de leite (Silva et al 2007, Biasutti et al 2008, Souza et al 2008) e concentrado proteico de soro de leite (Afonso et al 2008(Afonso et al e 2009.…”

Recent development in derivative ultraviolet/visible absorption spectrophotometry: 2004–2008

“…Effects on physical properties of liposome membranes; partition coefficient for dodecylbenzene sulphonate and Triton X-100 by DS [220] N-Sulphamoyloxazolidinones Study of inclusion complexes with ␤-cyclodextrin; stoichiometries and stability constants at various pHs determined using 2nd derivative [221] Pancreatin Immobilization in activated carbon and in alumina for preparing whey hydrolysates; 2nd derivative for evaluation of phenylalanine exposition degree used for determination of the enzyme operational stability [222] Papain Immobilized on activated carbon and on alumina; quantification of exposure rate of phenylalanine by 2nd derivative used to determine the enzyme activity [223] Phenylalanine Testing several conditions using activated carbon for removing phenylalanine from protein hydrolysates; 2nd derivative for evaluate the efficiency [224] Phenylalanine (Phe) Optimization of enzyme assisted processes for extracting and hydrolysing corn proteins aiming phenylalanine removal; efficiency of removal evaluated by 2nd derivative measuring Phe content in corn flour as well as in hydrolysates after activated carbon treatment [225] Protein Probing structure and dynamics by 2nd derivative ultraviolet absorption analysis of cation-interactions [226] Amipicilin…”