Influence of calcium content of tissue on hyperhydricity and shoot-tip necrosis of in vitro regenerated shoots of Lavandula angustifolia Mill.

Machado, Marília Pereira; Silva, André Luís Lopes da; Biasi, Luiz Antônio; Deschamps, Cícero; Filho, João Carlos Bespalhok; Zanette, Flávio

doi:10.1590/s1516-8913201402165

Cited by 31 publications

(24 citation statements)

References 23 publications

(17 reference statements)

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“…Callus initiation was achieved from the explants within 25-30 days of inoculation ( Figure 2B). Both types of selected explants gave the maximum percentage of callusing, which confirms previous studies on various plants including Mentha spicata (Poovaiah et al 2006), Thymus hyemalis (Nordine et al 2014), Lavandula angustifolia (Machado et al 2014), and Artemisia pallens (Nathar and Yatoo 2014). All types of media produced callus on both explants except PGRs-free medium (control), which did not produce callus on leaf explants.…”

Section: Resultssupporting

confidence: 87%

In vitro callus induction and micropropagation of Thymus persicus (Lamiaceae), an endangered medicinal plant

Bakhtiar

Mirjalili

Sonboli

2016

Crop Breed. Appl. Biotechnol.

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Section: Resultssupporting

confidence: 87%

In vitro callus induction and micropropagation of Thymus persicus (Lamiaceae), an endangered medicinal plant

Bakhtiar

Mirjalili

Sonboli

2016

Crop Breed. Appl. Biotechnol.

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“…Calcium deficiency is wellknown in the hyperhydric tissues of Dianthus caryophyllus (Kevers and Gaspar 1986) and Petunia hybrida (Zimmerman et al, 1988). Machado et al (2014) demonstrated that the addition of calcium chloride (1.32 g · L -1 ) to the MS culture medium reduced hyperhydricity in Lavendula angustifolia shoots from 23% and 30% to 6% and 1.3% in the first and second subcultures, respectively. Similar findings in Cydonia oblonga (Singha et al, 1990) and Solanum tuberosum (Sha et al, 1985) indicated that increases in calcium improve plant growth and reduce or eliminate deformities such as hyperhydricity and shoot tip necrosis in cultures.…”

Section: Resultsmentioning

confidence: 99%

Micropropagation of Banana: Reversion, Rooting, and Acclimatization of Hyperhydric Shoots

El-Mahrouk¹,

El-Shereif²,

Dewir³

et al. 2019

horts

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Hyperhydricity is a physiological disorder impacting plant growth and multiplication and acclimatization of regenerated plantlets. We report the use of calcium nitrate for reversion and acclimatization of banana ‘Grand Naine’ hyperhydric shoots cultured on Murashige and Skoog medium containing agar or gellan. Although 100% rooting of hyperhydric shoots occurred at all concentrations of calcium nitrate, only 50% rooting was recorded in the absence of calcium nitrate. Electrolyte leakage decreased significantly in the reverted banana tissues compared with the hyperhydric tissues. Histochemical staining for reactive oxygen species indicated that reverted banana tissues possess lower levels of both hydrogen peroxide (H2O2) and superoxide (O2-) than do hyperhydric tissues. Rooting, growth, and survival of the reverted banana plantlets were significantly influenced by calcium nitrate concentrations as well as the type of gelling agent. Reverted banana plantlets in medium containing calcium nitrate (0.5–1 g·L−1) were acclimatized with 100% survival in a growing substrate of peatmoss and vermiculite (1:1).

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“…On the other hand, Machado et al (2014) had noted that the number of trichomes in L. angustifolia was lower on both the abaxial and adaxial sides of the leaf of in vitro plants compared with the leaves of field-grown plants.…”

Section: Micromorphological Analysismentioning

confidence: 99%

Antioxidant and antimicrobial activities of Lavandula angustifolia Mill. field-grown and propagated in vitro

Andrys

Kulpa

Grzeszczuk

et al. 2017

Folia Horticulturae

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In the study, micropropagation of three varieties of Lavandula angustifolia was developed, and the appearance of trichomes, antioxidant activity of extracts and antimicrobial activity of essential oils isolated from plants growing in field conditions and in vitro cultures were compared. The study evaluated the number of shoots, and the height and weight of the plants grown on media with additions of BAP, KIN and 2iP. The greatest height was attained by the lavenders growing on MS medium with the addition of 1 mg dm -3 2iP -'Ellagance Purple'. The greatest number of shoots was developed by the 'Ellagance Purple' and 'Munstead' plants growing on the medium with 2 mg dm -3 BAP. The highest weight was attained by the plants growing on the medium with the highest concentration of BAP -3 and 5 mg dm . Moreover, the present study determined the influence of media with the addition of different concentrations of IBA and media with a variable mineral composition (½, ¼, and complete composition of MS medium) and with the addition of IBA or NAA for rooting. The majority of the media used had a positive influence on the development of the root system. The longest root system was observed in 'Ellagance Purple' growing on the medium composed of ¼ MS with 0.2 mg dm -3 NAA. All the examined oils exhibited activity towards S. aureus, S. epidermidis, P. aeruginosa, E. coli and C. albicans. The majority of the essential oils isolated from the plants propagated in vitro exhibited stronger antimicrobial activity than the field-grown plants. The plants propagated under in vitro conditions demonstrated considerably higher antioxidant activity as compared with the field-grown plants, which was determined using the DPPH, FRAP and ABTS assay.

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Influence of calcium content of tissue on hyperhydricity and shoot-tip necrosis of in vitro regenerated shoots of Lavandula angustifolia Mill.

Abstract: ABSTRACT

Cited by 31 publications

References 23 publications

In vitro callus induction and micropropagation of Thymus persicus (Lamiaceae), an endangered medicinal plant

In vitro callus induction and micropropagation of Thymus persicus (Lamiaceae), an endangered medicinal plant

Micropropagation of Banana: Reversion, Rooting, and Acclimatization of Hyperhydric Shoots

Antioxidant and antimicrobial activities of Lavandula angustifolia Mill. field-grown and propagated in vitro

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