Micropropagation of Banana: Reversion, Rooting, and Acclimatization of Hyperhydric Shoots

El-Mahrouk, Mohammed E.; El-Shereif, Ali R.; Dewir, Yaser Hassan; Hafez, Y. M.; Abdelaal, Kh. A. A.; El-Hendawy, Salah; Migdadi, Hussein M.; Al-Obeed, R. S.

doi:10.21273/hortsci14036-19

Cited by 7 publications

(4 citation statements)

References 42 publications

(68 reference statements)

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“…These ROS have the ability to damage the nucleic acids, cause the denaturation of proteins and lipids, and, ultimately, the death of the cells [ 5 ]. Many studies confirmed the increase in EL due to stresses on banana plants such as salinity, drought stress [ 41 ], and the stress of copper sulphate as presented in the current study, whereas other studies reported that EL decreased due to calcium nitrate (0.5 to 1.0 g L –1 ) in banana plantlets [ 42 ].…”

Section: Resultssupporting

confidence: 82%

“…The stress of copper sulphate was also reported by some studies using different concentrations of up to 100 µM [ 17 ], and up to 8 mg L −1 [ 25 ]. The previous trend was not common for some other elements like calcium and silicon, which increased the chlorophyll content in banana plantlets when added at certain concentrations, such as with 0.5 g L −1 of calcium nitrate [ 42 ] and 1 g L −1 in the form of CaSiO 3 [ 43 ].…”

Section: Resultsmentioning

confidence: 99%

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In Vitro Propagation and Acclimatization of Banana Plants: Antioxidant Enzymes, Chemical Assessments and Genetic Stability of Regenerates as a Response to Copper Sulphate

Elyazid

Salama

Zanaty

et al. 2021

Plants

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Developing a successful protocol for banana in vitro culture is a guarantee for the mass propagation of pathogen-free, high-quality, true-to-type planting materials with low production costs. The current work aimed to investigate the influence of increasing copper levels in an MS medium on endophytic bacterial contamination; shoot multiplication; rooting and the acclimatization of in vitro cultured banana; minerals and chlorophyll content; antioxidant enzymes activity; electrolyte leakage; and the genetic stability of banana regenerants. Four different concentrations of copper sulphate (0.025 as a control, and 30, 60, and 120 mg L−1) were examined. The growth of the endophytic bacteria was inhibited at 60 mg L−1 of copper sulphate which recorded zero contamination, without a significant difference at 120 mg L−1. However, 0.025 mg L−1 of copper sulphate was optimal for the maximum shoot number and shoot length (10 shoots and 6 cm, respectively) without significant differences at 30 mg L−1. The root length of banana plantlets was significantly enhanced at 30 mg L−1 of copper sulphate but without significant differences to the control, regarding the number of roots (9.92 cm and 3.80 roots, respectively). In vitro plants were acclimatized successfully at 30 mg L−1 of copper sulphate with 100% survival. The uptake of minerals, antioxidant enzyme activity and electrolyte leakage was improved because of the copper sulphate, but the chlorophyll level decreased. RAPD profiling showed polymorphism in only one plant treated with 60 mg L−1 of copper sulphate, with an average of 1.8%. The genome template stability percentage was almost 100% for all treated plants.

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Section: Resultssupporting

confidence: 82%

Section: Resultsmentioning

confidence: 99%

In Vitro Propagation and Acclimatization of Banana Plants: Antioxidant Enzymes, Chemical Assessments and Genetic Stability of Regenerates as a Response to Copper Sulphate

Elyazid

Salama

Zanaty

et al. 2021

Plants

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“…Since 1982, Pleioblastus pygmaeus has been one of the oldest species at the Bamboo Garden of Nanjing Forestry University in Nanjing, Jiangsu, China. In the pre-treatment stage, 10 mm long nodal explants were cultured in a MS medium [ 37 , 38 ]. Hence, the bamboo shoots were initially formed and grown in the MS medium containing 0.6 mL kinetin (KT), 8–12 g L −1 agar, and 28 g L −1 sucrose.…”

Section: Methodsmentioning

confidence: 99%

Benzylaminopurine and Abscisic Acid Mitigates Cadmium and Copper Toxicity by Boosting Plant Growth, Antioxidant Capacity, Reducing Metal Accumulation and Translocation in Bamboo [Pleioblastus pygmaeus (Miq.)] Plants

et al. 2022

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An in vitro experiment was conducted to determine the influence of phytohormones on the enhancement of bamboo resistance to heavy metal exposure (Cd and Cu). To this end, one-year-old bamboo plants (Pleioblastus pygmaeus (Miq.) Nakai.) contaminated by 100 µM Cd and 100 µM Cu both individually and in combination were treated with 10 µM, 6-benzylaminopurine and 10 µM abscisic acid. The results revealed that while 100 µM Cd and 100 µM Cu accelerated plant cell death and decreased plant growth and development, 10 µM 6-benzylaminopurine and 10 µM abscisic acid, both individually and in combination, increased plant growth by boosting antioxidant activities, non-antioxidants indices, tyrosine ammonia-lyase activity (TAL), as well as phenylalanine ammonia-lyase activity (PAL). Moreover, this combination enhanced protein thiol, total thiol, non-protein, glycine betaine (GB), the content of proline (Pro), glutathione (GSH), photosynthetic pigments (Chlorophyll and Carotenoids), fluorescence parameters, dry weight in shoot and root, as well as length of the shoot. It was then concluded that 6-benzyl amino purine and abscisic acid, both individually and in combination, enhanced plant tolerance under Cd and Cu through several key mechanisms, including increased antioxidant activity, improved photosynthesis properties, and decreased metals accumulation and metal translocation from root to shoot.

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“…There are two strategies that could be applied to control hyperhydricity: preventing the incidence of hyperhydricity, and the reversion of hyperhydric plantlets to their normal appearance. The majority of the previous literature focused on the prevention the occurrence of hyperhydricity in advance, while a few studies reported the reversion of hyperhydric shoots to their normal growth in order to improve the performance of in vitro propagation of some species, including the banana plant [ 19 ]; Agave sisalana Perr. [ 20 ]; and Dianthus chinensis L. [ 17 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Optimizing Medium Composition and Environmental Culture Condition Enhances Antioxidant Enzymes, Recovers Gypsophila paniculata L. Hyperhydric Shoots and Improves Rooting In Vitro

Mohamed

El-Mahrouk

El-Banna

et al. 2023

Plants

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Gypsophila paniculata L. is one of the most important commercial cut flowers worldwide. The plant is sterile and propagated mainly by in vitro culture techniques. However, hyperhydricity hinders its micropropagation and increases mortality during ex vitro acclimatization. Hyperhydric shoots of G. paniculata were proliferated from nodal explants on MS medium without growth regulators that contained 30 g L−1 sucrose, and gelled with 6.0 g L−1 agar. Medium components and environmental culture conditions were optimized to revert hyperhydricity in G. paniculata microshoots and develop an efficient micropropagation protocol for commercial production. Multiple shoots with high quality were successfully regenerated on MS medium fortified with potassium and ammonium nitrate at full concentration, 2.0 mg L−1 paclobutrazol, solidified with 9.0 g L−1agar in Magenta boxes of 62.87 gas exchange/day and incubated under light density of 60 µmol m−2s−1. We recorded 4.33 shoots, 40.00 leaves, 6.33 cm, 2.50 g and 95.00% for number of shoots/explant, number of leaves/shoot, shoot length, shoot fresh weight and normal shoots percentage, respectively. Well-rooted plantlets of G. paniculata were developed from the reverted microshoots, with the rooting percentage (95.00%) on MS medium augmented with 1.0 mg L−1 IBA in Magenta boxes of 62.87 gas exchange/day and 60 µmol m−2s−1 light density. In vitro-rooted plantlets exhibited reduced electrolyte leakage, and enhanced antioxidant enzymes activity of peroxidase, catalase, and polyphenol oxidase due to good ventilation at the highest gas exchange rate of the culture vessels.

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Micropropagation of Banana: Reversion, Rooting, and Acclimatization of Hyperhydric Shoots

Cited by 7 publications

References 42 publications

In Vitro Propagation and Acclimatization of Banana Plants: Antioxidant Enzymes, Chemical Assessments and Genetic Stability of Regenerates as a Response to Copper Sulphate

In Vitro Propagation and Acclimatization of Banana Plants: Antioxidant Enzymes, Chemical Assessments and Genetic Stability of Regenerates as a Response to Copper Sulphate

Benzylaminopurine and Abscisic Acid Mitigates Cadmium and Copper Toxicity by Boosting Plant Growth, Antioxidant Capacity, Reducing Metal Accumulation and Translocation in Bamboo [Pleioblastus pygmaeus (Miq.)] Plants

Optimizing Medium Composition and Environmental Culture Condition Enhances Antioxidant Enzymes, Recovers Gypsophila paniculata L. Hyperhydric Shoots and Improves Rooting In Vitro

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